Simultaneous determination of antiretroviral zidovudine, lamivudine and efavirenz by RP HPLC-DAD
A reverse phase high-performance liquid chromatography-photodiode array detector (RP HPLC-DAD) method for simultaneous determination of lamivudine (3TC), zidovudine (AZT), two nucleoside reverse transcriptase inhibitors, and efavirenz (EFV), a non-nucleoside reverse transcriptase inhibitor, in table...
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| Autores principales: | , , , , , |
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| Formato: | Articulo |
| Lenguaje: | Inglés |
| Publicado: |
2011
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| Materias: | |
| Acceso en línea: | http://sedici.unlp.edu.ar/handle/10915/8134 http://www.latamjpharm.org/resumenes/30/2/LAJOP_30_2_1_9.pdf |
| Aporte de: |
| Sumario: | A reverse phase high-performance liquid chromatography-photodiode array detector (RP HPLC-DAD) method for simultaneous determination of lamivudine (3TC), zidovudine (AZT), two nucleoside reverse transcriptase inhibitors, and efavirenz (EFV), a non-nucleoside reverse transcriptase inhibitor, in tablets is described. The drugs separation was performed on a C18 column (250 x 4,6 mm, 5 μm - Phenomenex®), through a mobile phase drag composed by a binary gradient of acetonitrile and water HPLC grade. The drugs detection was performed at 248 nm, resulting in a 14 min chromatographic run. The samples were prepared by crushing tablets, weighing powder, diluting it by a 10 min sonication and filtrating solution, resulting in samples with final concentration of 20, 40 e 40 μg.mL–1 of 3TC, AZT and EFV, respectively. The method was adequate for quality control purposes and was validated following the ICH guidelines. |
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