Determination of dextromethorphan in rabbit plasma by LC-MS/MS and its application to pharmacokinetic study
A highly sensitive liquid chromatographic tandem mass spectrometric (LC-MS/MS) method for determination of dextromethorphan in rabbit plasma using triazolam as the internal standard (IS) was developed. Plasma samples were extracted with ethyl acetate and separated on a SB-C18 column with a mobile ph...
Guardado en:
| Autores principales: | , , , , , |
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| Formato: | Articulo Comunicacion |
| Lenguaje: | Inglés |
| Publicado: |
2010
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| Materias: | |
| Acceso en línea: | http://sedici.unlp.edu.ar/handle/10915/7994 http://www.latamjpharm.org/resumenes/29/5/LAJOP_29_5_2_12.pdf |
| Aporte de: |
| id |
I19-R120-10915-7994 |
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| record_format |
dspace |
| institution |
Universidad Nacional de La Plata |
| institution_str |
I-19 |
| repository_str |
R-120 |
| collection |
SEDICI (UNLP) |
| language |
Inglés |
| topic |
Farmacia dextromethorphan; LC-MS/MS; determination; rabbit; pharmacokinetic Medicamentos Farmacología Farmacocinética |
| spellingShingle |
Farmacia dextromethorphan; LC-MS/MS; determination; rabbit; pharmacokinetic Medicamentos Farmacología Farmacocinética Hu, Lufeng Wang, Xianqin Jiang, Haiyan Xu, Xuegu Xu, Ren-ai Lin, Guanyang Determination of dextromethorphan in rabbit plasma by LC-MS/MS and its application to pharmacokinetic study |
| topic_facet |
Farmacia dextromethorphan; LC-MS/MS; determination; rabbit; pharmacokinetic Medicamentos Farmacología Farmacocinética |
| description |
A highly sensitive liquid chromatographic tandem mass spectrometric (LC-MS/MS) method for determination of dextromethorphan in rabbit plasma using triazolam as the internal standard (IS) was developed. Plasma samples were extracted with ethyl acetate and separated on a SB-C18 column with a mobile phase of acetonitrile-water 60:40 (v/v) at a flow of 0.3 mL/min. Detection is carried out by multiple reaction monitoring (MRM) on a ion-trap LC-MS/MS system with an electrospray ionization interface. The lower limit of quantification (LLOQ) was 1 ng/mL. After intravenous administration of a single dose of dextromethorphan 2 mg/kg, the main pharmacokinetic parameters were as follows: AUC<sub>0→t</sub> 636.13 ± 47.13 (ng/mL·h); CL 2.60 ± 0.24 (L/h), C<sub>max</sub> 874 ± 67.16 (ng/mL), Vz 1.58 ± 0.11 (L/kg), T1/2 2.41 ± 0.35 (h), MRT 1.26 ± 0.08 (h). |
| format |
Articulo Comunicacion |
| author |
Hu, Lufeng Wang, Xianqin Jiang, Haiyan Xu, Xuegu Xu, Ren-ai Lin, Guanyang |
| author_facet |
Hu, Lufeng Wang, Xianqin Jiang, Haiyan Xu, Xuegu Xu, Ren-ai Lin, Guanyang |
| author_sort |
Hu, Lufeng |
| title |
Determination of dextromethorphan in rabbit plasma by LC-MS/MS and its application to pharmacokinetic study |
| title_short |
Determination of dextromethorphan in rabbit plasma by LC-MS/MS and its application to pharmacokinetic study |
| title_full |
Determination of dextromethorphan in rabbit plasma by LC-MS/MS and its application to pharmacokinetic study |
| title_fullStr |
Determination of dextromethorphan in rabbit plasma by LC-MS/MS and its application to pharmacokinetic study |
| title_full_unstemmed |
Determination of dextromethorphan in rabbit plasma by LC-MS/MS and its application to pharmacokinetic study |
| title_sort |
determination of dextromethorphan in rabbit plasma by lc-ms/ms and its application to pharmacokinetic study |
| publishDate |
2010 |
| url |
http://sedici.unlp.edu.ar/handle/10915/7994 http://www.latamjpharm.org/resumenes/29/5/LAJOP_29_5_2_12.pdf |
| work_keys_str_mv |
AT hulufeng determinationofdextromethorphaninrabbitplasmabylcmsmsanditsapplicationtopharmacokineticstudy AT wangxianqin determinationofdextromethorphaninrabbitplasmabylcmsmsanditsapplicationtopharmacokineticstudy AT jianghaiyan determinationofdextromethorphaninrabbitplasmabylcmsmsanditsapplicationtopharmacokineticstudy AT xuxuegu determinationofdextromethorphaninrabbitplasmabylcmsmsanditsapplicationtopharmacokineticstudy AT xurenai determinationofdextromethorphaninrabbitplasmabylcmsmsanditsapplicationtopharmacokineticstudy AT linguanyang determinationofdextromethorphaninrabbitplasmabylcmsmsanditsapplicationtopharmacokineticstudy |
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Repositorios |
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1764820487345537026 |