Determination of dextromethorphan in rabbit plasma by LC-MS/MS and its application to pharmacokinetic study
A highly sensitive liquid chromatographic tandem mass spectrometric (LC-MS/MS) method for determination of dextromethorphan in rabbit plasma using triazolam as the internal standard (IS) was developed. Plasma samples were extracted with ethyl acetate and separated on a SB-C18 column with a mobile ph...
Guardado en:
| Autores principales: | , , , , , |
|---|---|
| Formato: | Articulo Comunicacion |
| Lenguaje: | Inglés |
| Publicado: |
2010
|
| Materias: | |
| Acceso en línea: | http://sedici.unlp.edu.ar/handle/10915/7994 http://www.latamjpharm.org/resumenes/29/5/LAJOP_29_5_2_12.pdf |
| Aporte de: |
| Sumario: | A highly sensitive liquid chromatographic tandem mass spectrometric (LC-MS/MS) method for determination of dextromethorphan in rabbit plasma using triazolam as the internal standard (IS) was developed. Plasma samples were extracted with ethyl acetate and separated on a SB-C18 column with a mobile phase of acetonitrile-water 60:40 (v/v) at a flow of 0.3 mL/min. Detection is carried out by multiple reaction monitoring (MRM) on a ion-trap LC-MS/MS system with an electrospray ionization interface. The lower limit of quantification (LLOQ) was 1 ng/mL. After intravenous administration of a single dose of dextromethorphan 2 mg/kg, the main pharmacokinetic parameters were as follows: AUC<sub>0→t</sub> 636.13 ± 47.13 (ng/mL·h); CL 2.60 ± 0.24 (L/h), C<sub>max</sub> 874 ± 67.16 (ng/mL), Vz 1.58 ± 0.11 (L/kg), T1/2 2.41 ± 0.35 (h), MRT 1.26 ± 0.08 (h). |
|---|