Simple high performance liquid chromatography method for determination of benzalkonium chloride homologues in nasal preparations
A High-Performance Liquid Chromatography method was developed for quantification of benzalkonium chloride (BAC) and separation of three major homologues, in nasal preparations. Chromatography separation was carried out on a CLC-cyano column, using 0.075M acetate buffer with acetonitrile (45:55, v/v)...
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| Autores principales: | , , , , |
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| Formato: | Articulo |
| Lenguaje: | Inglés |
| Publicado: |
2009
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| Materias: | |
| Acceso en línea: | http://sedici.unlp.edu.ar/handle/10915/7760 http://www.latamjpharm.org/trabajos/28/2/LAJOP_28_2_2_4_8919RZR280.pdf |
| Aporte de: |
| Sumario: | A High-Performance Liquid Chromatography method was developed for quantification of benzalkonium chloride (BAC) and separation of three major homologues, in nasal preparations. Chromatography separation was carried out on a CLC-cyano column, using 0.075M acetate buffer with acetonitrile (45:55, v/v) pH = 5.0, as mobile phase, in an isocratic elution. The total run time was 20 min at a flow rate of 1.0 mL/min. The retention times were of 10.0, 12.8 and 16.1 min, respectively, for homologs C12 , C14 and C16 of benzalkonium chloride. About precision, the method presented excellent repeatability and satisfactory precision intermediate, and the coefficients of variation found were 1.73 and 4.51%, respectively. The accuracy values were ranging from 100.86 to 110.99%. The lower limits of quantification and detection were 5 μg/mL and 2.5 μg/mL, respectively. Two out of seven analyzed lots contained less BAC than allowed. |
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