Cytotoxicity of copper and silver ions on specific osteoblastic properties
Copper and silver are widely used as components of dental materials even though their corrosion resistance is low. The released ions can produce several undesirable effects such as toxic, allergic, inflammatory and mutagenic reactions. The aim of the present study was to evaluate the biocompatibilit...
Guardado en:
| Autores principales: | , , |
|---|---|
| Formato: | Articulo Comunicacion |
| Lenguaje: | Inglés |
| Publicado: |
2006
|
| Materias: | |
| Acceso en línea: | http://sedici.unlp.edu.ar/handle/10915/71078 https://digital.cic.gba.gob.ar/handle/11746/4522 |
| Aporte de: |
| Sumario: | Copper and silver are widely used as components of dental materials even though their corrosion resistance is low. The released ions can produce several undesirable effects such as toxic, allergic, inflammatory and mutagenic reactions. The aim of the present study was to evaluate the biocompatibility of the components of dental metal materials on the growth and differentiation of UMR106 osteoblast cells in response to salt solutions of the metallic ions (Cu2+ and Ag+) and to ions released from pure metals. The control UM106 cells exhibited normal polygonal morphology with cytoplasmic processes connecting neighbouring cells. After 24 h in culture, cells exposed to Cu showed important morphological changes. After a longer incubation (48 h) the copper ions induced almost a complete cellular death of the culture. Exposition to silver for 48 h caused a lower reduction in the healthy cells in the culture. Osteoblasts were relatively small with lost of processes, although some mitotic figures and apoptotic bodies were still observed. The effect of Cu on the inhibition of cell growth was linearly time-dependent. Both cell surviving and the MI, significantly correlated with the Cu levels in the media, although this correlation was stronger when the number of surviving cells was analysed, in comparison with the mitotic index. It could be concluded that copper and silver ions are cytotoxic. In the case of copper ions a second order correlation between cytotoxicity and the concentration of the released metal ions was found. |
|---|