HLA-DQA1 allele typing by nonisotopic PCR-LIS-SSCP

In the present study we used a simple and reliable method for HLA-DQA1 allele typing based on the single-stranded conformation polymorphism (SSCP) properties of DNA molecules obtained by PCR. The technique consists of PCR amplification of a DNA fragment comprising the second exon of the HLA-DQA1 gen...

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Detalles Bibliográficos
Autores principales: Abba, Martín Carlos, Gómez, María Atilia, Golijow, Carlos Daniel
Formato: Articulo
Lenguaje:Inglés
Publicado: 2001
Materias:
Ciencias Veterinarias
agar gel electrophoresis
HLA-DQA1
LIS-SSCP
genetics
PCR
osmolarity
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/37574
http://www.scielo.br/pdf/bjmbr/v34n7/4121.pdf
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata

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