Determinación de contenido fenólico total en agua superficial de distintos puntos de la provincia de Santa Fe – Argentina – haciendo uso de un biosensor enzimático mediante calibración multivariada por cuadrados parciales mínimos, PLS
Phenolic compounds are a group of organic pollutants present in the environment as a result of various processes such as industrial, biogeochemical and as pesticide degradation products. Due to their toxicity and persistence, a number of phenolic compounds have been included in the international leg...
Guardado en:
| Autores principales: | , , |
|---|---|
| Formato: | Objeto de conferencia |
| Lenguaje: | Español |
| Publicado: |
2012
|
| Materias: | |
| Acceso en línea: | http://sedici.unlp.edu.ar/handle/10915/26501 http://www.congresos.unlp.edu.ar/index.php/CCMA/7CCMA/paper/viewFile/1105/303 |
| Aporte de: |
| Sumario: | Phenolic compounds are a group of organic pollutants present in the environment as a result of various processes such as industrial, biogeochemical and as pesticide degradation products. Due to their toxicity and persistence, a number of phenolic compounds have been included in the international legislation. Numerous analytical methodologies have been developed for detecting phenolic compounds, for example, ultraviolet spectrophotometric analyses, gas chromatography, liquid chromatography, and capillary electrophoresis. However, these were time consuming, complex to perform, require samples pre-treatment, and may not be suitable for in situ monitoring.
Recently, a large number of efforts have been made for the simple and effective determination of phenolic compounds. Biosensors represent a potential screening method in environmental studies, for instance in the analysis of phenolic compounds.
As an analytical detector, biosensors have advantageous properties such as high selectivity and sensitivity. The production cost is also relatively low and the analysis time is short compared to conventional analytical methods.
In this work, the dynamic peak responses from a single amperometric tyrosinase-based sensor are used with multivariate data analysis for quantitative determination of catechol p-chlorophenol,phenol and cresol in water samples. Partial least square regression (PLS) was utilized in order to resolve hard overlapped electrochemical signals. Measurements were made with a composite graphite electrode modified with tyrosinase enzyme and Nafion at an applied potential of – 0.1 V versus Ag/AgCl by square wave voltammetry (SWV) technique. All experiments were performed at the temperature of 30°C, in the electrochemical cell containing phosphate buffer at pH=6.80. Several groundwater and superficial water samples belonging to localities of Santa Fe were assayed. All the samples were containing catechol and p-clorophenol but they were not phenol and cresols. |
|---|