Determinación de atrazina y los compuestos intermediarios a su biodegradación en un medio de cultivo líquido para el enriquecimiento de consorcios microbianos
1-chloro-3-ethylamino-5-isopropylamino-2 ,4,6-triazine, is an herbicide used to control annual weeds and perennial grasses. Although this herbicide is banned in the European Union for its toxicity, it is still widely used. Also, its the second most frecuently employed in Uruguay. The main mechanism...
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| Autores principales: | , , , , |
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| Formato: | Objeto de conferencia |
| Lenguaje: | Español |
| Publicado: |
2012
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| Acceso en línea: | http://sedici.unlp.edu.ar/handle/10915/26499 http://www.congresos.unlp.edu.ar/index.php/CCMA/7CCMA/paper/viewFile/905/205 |
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| Sumario: | 1-chloro-3-ethylamino-5-isopropylamino-2 ,4,6-triazine, is an herbicide used to control annual weeds and perennial grasses. Although this herbicide is banned in the European Union for its toxicity, it is still widely used. Also, its the second most frecuently employed in Uruguay.
The main mechanism for removal of atrazine in neutral pH environments is the bacterial degradation. The microorganisms can mineralize atrazine giving ammonia and carbon dioxide or they can degrade it to intermediates which vary in persistence and toxicity.
The separation and detection of atrazine intermediates is important to know the health risk that represents its partial degradation in the environment. Furthermore, the separation method could be usefull to characterize bacterial degraders with potential application in biorremediation. Among the atrazine biodegradation intermediates are the hidroxyatrazine, the desetylhidroxyatrazine, the desisopropylhidrtoxyatrazine and cyanuric acid.
In this paper we developed a method to separate atrazine and metabolites in a synthetic culture medium used to select atrazine degrading bacteria. For this technique we optimized a high performance liquid chromatography (HPLC) ion pair method using an C8 column, mobile phase acetonitrile / water, gradient flow and an UV detector. We evaluated its application to detect intermediates in several bacterial enrichments. It was found that the method is suitable to distinguish different metabolic capabilities of these enrichment. |
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