Capacidad de los basidiomicetes para degradar Endosulfán y Clorpirifón en una matriz compleja
The white and brown wood rotting fungi have shown a great ability to degrade xenobiotic compounds. These properties are determined by their battery of extracellular enzymes that can convert these compounds to CO2 and H2O. Compounds such as endosulfan and chlorpyrifos are important in Uruguay as they...
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| Autores principales: | , , , , , |
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| Formato: | Objeto de conferencia |
| Lenguaje: | Español |
| Publicado: |
2012
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| Materias: | |
| Acceso en línea: | http://sedici.unlp.edu.ar/handle/10915/26438 http://www.congresos.unlp.edu.ar/index.php/CCMA/7CCMA/paper/viewFile/921/212 |
| Aporte de: |
| Sumario: | The white and brown wood rotting fungi have shown a great ability to degrade xenobiotic compounds. These properties are determined by their battery of extracellular enzymes that can convert these compounds to CO2 and H2O. Compounds such as endosulfan and chlorpyrifos are important in Uruguay as they are extensively used in crops such as soybean and cereals, fruits, among others. These compounds are considered recalcitrant, and in Uruguay there is not still any environmentally friendly elimination procedure in use. Therefore, bioremediation of these compounds by Basidiomycetes arises as an adequate alternative. Two strains of basidiomycetes were selected after a screening study where the bioconversion ability of 11 strains from the Department of Microbiology was assessed. From the fungi studied, three showed a promising degradation capacity, and Abortiporus biennis and Bjerkandera adusta were chosen to continue the degradation study. We evaluated the ability of these Basidiomycetes to grow in plates containing 50 and 60 mg / kg of endosulfan and chlorpyrifos individually and as a mixture, in YNB medium with and without glucose, alternating the carbon source and the compound(s) of interest. Once it was demonstrated the ability to grow in the presence of high concentrations of such pesticides, we tested the biodegradation ability of these fungi in a matrix containing rice straw immersed in semisolid agar. This parameter was evaluated after 21 and 60 days of incubation. The percentage of degradation at 60 days was 39 and 33% for α and β endosulfan and 24% for chlorpyrifos. Acetonitrile was used as the extraction solvent and the clean-up procedure was a d-SPE (dispersive Solid Phase Extraction). Detection was performed by gas chromatography with electron capture detector. |
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