Biorremediación de suelos y aguas contaminadas con cobre : Cepas mutantes de Escherichia coli presentan diferente capacidad depuradora del metal

Copper is an essential nutrient for organisms but when in excess, it becomes potentially toxic. The copper contamination of soils, rivers and deep water has increased dangerously. Bacteria, algae and fungi are receiving increased attention for removing and/or recovery heavy metals due to its effecti...

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Autores principales: Lorenzetti, Yanina, Grillo-Puertas, Mariana, Scaravaglio, Omar R., Cerioni, Luciana, Volentini, Sabrina I., Rodríguez Montelongo, Luisa
Formato: Objeto de conferencia
Lenguaje:Español
Publicado: 2012
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Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/26429
http://www.congresos.unlp.edu.ar/index.php/CCMA/7CCMA/paper/viewFile/1088/365
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Sumario:Copper is an essential nutrient for organisms but when in excess, it becomes potentially toxic. The copper contamination of soils, rivers and deep water has increased dangerously. Bacteria, algae and fungi are receiving increased attention for removing and/or recovery heavy metals due to its effectiveness, low cost and easy availability. In our laboratory it was demonstrated that certain strains of Escherichia coli might detoxify liquid media with high concentrations of copper. The objectives of this study were: a) adequately process the soil samples, artificially contaminated with copper to release the metal of the liquid and then be captured by bacteria, b) using different mutants of E. coli to remediate the waste water from one of the steps in an sequential oxidative treatment (SOT) for controlling green mold in citrus, which contains in its composition a copper salt. a) Different soil samples artificially contaminated with copper were processed and the acid was obtained in better extraction was HCl. Three sequential extractions of 30 min each released about 90% of the metal. Strains of E. coli showed a higher capture of copper when the supernatants had a pH above 5. A single bacterial extraction recovered 90% of the metal. b) In solutions of SOT containing CuSO4, to recover 90% of the metal from it, were required perform three consecutive bacterial extractions. Some mutants in respiratory chain components were more effective than wild type in this process. This study confirms that some strains of E. coli, in an ex situ process could be efficiently used to detoxify soil or water contaminated with copper.