Identification of Chromones in the Seeds Extract of Saposhnikovia divaricata by Liquid Chromatography-Electrospray Ionization Mass Spectrometry
The present work describes a liquid chromatography-electrospray ionization mass spectrometry (LC-ESI-MS) method for rapid identification of four chromones, prim-O-glucosylcimifugin, 4’-O- β-D-glucosyl-5-O-methylvisamminol, cimifugin and 5-O-methylvisamminol in the seeds extract from Saposhnikovia...
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| Autores principales: | , , , , , |
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| Formato: | Articulo Comunicacion |
| Lenguaje: | Inglés |
| Publicado: |
2012
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| Materias: | |
| Acceso en línea: | http://sedici.unlp.edu.ar/handle/10915/18147 http://www.latamjpharm.org/resumenes/31/2/LAJOP_31_2_2_7.pdf |
| Aporte de: |
| Sumario: | The present work describes a liquid chromatography-electrospray ionization mass spectrometry (LC-ESI-MS) method for rapid identification of four chromones, prim-O-glucosylcimifugin, 4’-O-
β-D-glucosyl-5-O-methylvisamminol, cimifugin and 5-O-methylvisamminol in the seeds extract from
Saposhnikovia divaricata for the first time. By using a binary mobile phase system consisting of 0.5 %
acetic acid and acetonitrile under gradient conditions, a good separation was achieved in 25 min. The
[M+H]
+
ions, the [2M+Na]
+
ions, the molecular weights, and the fragment ions of the four chromones were
obtained in the positive ion mode using LC-ESI-MS. The identification of the chromones (peaks 1-4) in
seeds extract of S. divaricata was based on matching their retention times, the detection of molecular ions,
and the fragment ions obtained by LC-ESI-MS experiments with those of the authentic standards and data reported in the literature. |
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