An HPLC Method for the Determination of Auraptene in Dog Plasma: Application to Pharmacokinetic Study
A simple and sensitive high-performance liquid chromatography (HPLC) method was developed and validated for the determination of auraptene (AUR) in dog plasma. The chromatographic separation of AUR was achieved on a C18 column using isocratic elution with acetonitrile-0.1 % formic acid (volume rat...
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| Autores principales: | , , , , , , |
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| Formato: | Articulo |
| Lenguaje: | Inglés |
| Publicado: |
2012
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| Materias: | |
| Acceso en línea: | http://sedici.unlp.edu.ar/handle/10915/18099 http://www.latamjpharm.org/resumenes/31/2/LAJOP_31_2_1_12.pdf |
| Aporte de: |
| Sumario: | A simple and sensitive high-performance liquid chromatography (HPLC) method was developed and validated for the determination of auraptene (AUR) in dog plasma. The chromatographic separation of AUR was achieved on a C18 column using isocratic elution with acetonitrile-0.1 % formic acid
(volume ratio 84.5:15.5). AUR was detected at 322 nm. Sample extraction with ethyl acetate resulted in
high recoveries of AUR. A linear curve over the concentration range 10.2-408.8 ng/mL (r2
= 0.9995) was
obtained. Satisfactory intra-day and inter-day precisions were achieved with RSDs less than 9.5 % and the
average recovery factors were in the range of 99.1-106.2 %. The method was used to determine the plasma
concentration–time profiles for AUR after oral doses of 50, 100 and 200 mg/kg in dogs. A nonlinear pharmacokinetics was found in dogs at doses from 50 to 200 mg/kg. No significant accumulation of AUR in
dogs following multiple doses was observed. |
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