Self-assembled dithiothreitol on Au surfaces for biological applications: phospholipid bilayer formation

Self-assembly of dithiothreitol (DTT) on Au(111) from solution deposition has been studied by X-ray photoelectron spectroscopy and electrochemical data. DTT molecules self-assemble on Au(111) in a lying-down configuration irrespective of the concentration and temperature. XPS and electrochemical dat...

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Detalles Bibliográficos
Autores principales: Creczynski Pasa, Tânia B., Daza Millone, María Antonieta, Munford, Maximiliano L., De Lima, Vânia R., Vieira, Tiago O., Benitez, Guillermo Alfredo, Pasa, André A., Salvarezza, Roberto Carlos, Vela, María Elena
Formato: Articulo
Lenguaje:Inglés
Publicado: 2009
Materias:
Ciencias Exactas
Física
dithiothreitol
self-assembly
X-ray photoelectron spectroscopy
electrochemical data
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/162906
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
Descripción
Sumario:Self-assembly of dithiothreitol (DTT) on Au(111) from solution deposition has been studied by X-ray photoelectron spectroscopy and electrochemical data. DTT molecules self-assemble on Au(111) in a lying-down configuration irrespective of the concentration and temperature. XPS and electrochemical data indicate a DTT surface coverage of y E 0.16 with two S-head–Au covalent bonds per DTT molecule. The DTT monolayer turns the Au surface hydrophilic enough to allow the formation of fluid dimyristoylphosphatidylcholine (DMPC) bilayer domains by vesicle fusion as revealed by "in situ" atomic force imaging. Methylene blue (MB) and flavin adenine dinucleotide (FAD) have been used as probes to study molecule transport across the bilayer.

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