Development and validation of two SYBR green PCR assays and a multiplex real-time PCR for the detection of Shiga toxin-producing Escherichia coli in meat

Shiga toxin-producing Escherichia coli (STEC) are recognized as food-borne pathogens. We developed and validated two SYBR green PCR (SYBR-PCR) and a real-time multiplex PCR (RT-PCR) to detect stx1 and stx2 genes in meat samples, and compared these techniques in ground beef samples from retail stores...

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Detalles Bibliográficos
Autores principales: Brusa, Victoria, Galli, Lucía, Linares, Luciano Héctor, Ortega, Emanuel Eneas, Lirón, Juan Pedro, Leotta, Gerardo Aníbal
Formato: Articulo
Lenguaje:Inglés
Publicado: 2015
Materias:
Ciencias Veterinarias
Shiga toxin-producing Escherichia coli
SYBR-PCR
Rt-PCR
stx genes
Ground beef
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/154157
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata

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