Genotoxicity of the herbicide imazethapyr in mammalian cells by oxidative DNA damage evaluation using the Endo III and FPG alkaline comet assays

We evaluated the role of oxidative stress in the genotoxic damage induced by imazethapyr (IMZT) and its formulation Pivot® in mammalian CHO-K1 cell line. Using the alkaline comet assay, we observed that a concentration of 0.1 μg/mL of IMZT or Pivot® was able to induce DNA damage by increasing the fr...

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Autores principales: Soloneski, Sonia María Elsa, Ruiz de Arcaute, Celeste, Nikoloff, Noelia, Larramendy, Marcelo Luis
Formato: Articulo
Lenguaje:Inglés
Publicado: 2017
Materias:
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/141436
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id I19-R120-10915-141436
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Naturales
Comet assay
Imazethapyr-based formulation
Imidazolinone herbicides
Oxidative DNA damage
spellingShingle Ciencias Naturales
Comet assay
Imazethapyr-based formulation
Imidazolinone herbicides
Oxidative DNA damage
Soloneski, Sonia María Elsa
Ruiz de Arcaute, Celeste
Nikoloff, Noelia
Larramendy, Marcelo Luis
Genotoxicity of the herbicide imazethapyr in mammalian cells by oxidative DNA damage evaluation using the Endo III and FPG alkaline comet assays
topic_facet Ciencias Naturales
Comet assay
Imazethapyr-based formulation
Imidazolinone herbicides
Oxidative DNA damage
description We evaluated the role of oxidative stress in the genotoxic damage induced by imazethapyr (IMZT) and its formulation Pivot® in mammalian CHO-K1 cell line. Using the alkaline comet assay, we observed that a concentration of 0.1 μg/mL of IMZT or Pivot® was able to induce DNA damage by increasing the frequency of damaged nucleoids. To test whether the DNA lesions were caused by oxidative stress, the DNA repair enzymes endonuclease III (Endo III) and formamidopyrimidine-DNA glycosylase (Fpg), which convert base damage to strand breaks, were used. Our results demonstrate that after treatment of CHO-K1 cells with the pure active ingredient as well as the commercial formulation Pivot®, an increase in DNA strand breaks was observed after incubation of both Endo III and Fpg enzymes, indicating that both compounds induce DNA damage involving both pyrimidine and purine-based oxidations, at least in CHO-K1 cells. Our findings confirm the genotoxic potential of IMZT and suggest that this herbicide formulation must be employed with great caution, especially not only for exposed occupational workers but also for other living species.
format Articulo
Articulo
author Soloneski, Sonia María Elsa
Ruiz de Arcaute, Celeste
Nikoloff, Noelia
Larramendy, Marcelo Luis
author_facet Soloneski, Sonia María Elsa
Ruiz de Arcaute, Celeste
Nikoloff, Noelia
Larramendy, Marcelo Luis
author_sort Soloneski, Sonia María Elsa
title Genotoxicity of the herbicide imazethapyr in mammalian cells by oxidative DNA damage evaluation using the Endo III and FPG alkaline comet assays
title_short Genotoxicity of the herbicide imazethapyr in mammalian cells by oxidative DNA damage evaluation using the Endo III and FPG alkaline comet assays
title_full Genotoxicity of the herbicide imazethapyr in mammalian cells by oxidative DNA damage evaluation using the Endo III and FPG alkaline comet assays
title_fullStr Genotoxicity of the herbicide imazethapyr in mammalian cells by oxidative DNA damage evaluation using the Endo III and FPG alkaline comet assays
title_full_unstemmed Genotoxicity of the herbicide imazethapyr in mammalian cells by oxidative DNA damage evaluation using the Endo III and FPG alkaline comet assays
title_sort genotoxicity of the herbicide imazethapyr in mammalian cells by oxidative dna damage evaluation using the endo iii and fpg alkaline comet assays
publishDate 2017
url http://sedici.unlp.edu.ar/handle/10915/141436
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