Detection of excision repaired DNA damage in the comet assay by using Ara-C and hydroxyurea in three different cell types

Because of its characteristics, the comet assay has been used to evaluate the ability of virtually any type of eukaryotic cell to repair different kinds of DNA damage, including double and single strand breaks and base damage. The ability to detect excision repair sites using the alkaline version ca...

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Autores principales: Güerci, Alba Mabel, Liviac, D., Marcos, Ricard
Formato: Articulo
Lenguaje:Inglés
Publicado: 2009
Materias:
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/140052
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id I19-R120-10915-140052
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Naturales
Biología
Comet assay
Ara-C
Hydroxyurea
Alkylating agents
Human cell lines
spellingShingle Ciencias Naturales
Biología
Comet assay
Ara-C
Hydroxyurea
Alkylating agents
Human cell lines
Güerci, Alba Mabel
Liviac, D.
Marcos, Ricard
Detection of excision repaired DNA damage in the comet assay by using Ara-C and hydroxyurea in three different cell types
topic_facet Ciencias Naturales
Biología
Comet assay
Ara-C
Hydroxyurea
Alkylating agents
Human cell lines
description Because of its characteristics, the comet assay has been used to evaluate the ability of virtually any type of eukaryotic cell to repair different kinds of DNA damage, including double and single strand breaks and base damage. The ability to detect excision repair sites using the alkaline version can be enhanced by the inclusion of repair inhibitors, DNA synthesis inhibitors, or chain terminators. In this sense, we evaluated the ability of hydroxyurea (HU) and cytosine arabinoside (Ara-C), for detecting lesions produced by the alkylating agents ethyl methanesulfonate (EMS) and methyl methanesulfonate (MMS) in three different cell systems. Two hundred cells for experimental point were analyzed in the alkaline version of the comet assay, and the results are evidences of the utility of the assay to detect alkylation of bases in the cells lines MRC-5 and TK-6, as the treatment with HU +Ara-C significantly increases both the basal and induced frequency of DNA damage. The use of whole blood, although it detected the effects of MMS, with and without repair inhibitors, failed to detect the effect of the selected dose of EMS and does not permit detection increases in the background level.
format Articulo
Articulo
author Güerci, Alba Mabel
Liviac, D.
Marcos, Ricard
author_facet Güerci, Alba Mabel
Liviac, D.
Marcos, Ricard
author_sort Güerci, Alba Mabel
title Detection of excision repaired DNA damage in the comet assay by using Ara-C and hydroxyurea in three different cell types
title_short Detection of excision repaired DNA damage in the comet assay by using Ara-C and hydroxyurea in three different cell types
title_full Detection of excision repaired DNA damage in the comet assay by using Ara-C and hydroxyurea in three different cell types
title_fullStr Detection of excision repaired DNA damage in the comet assay by using Ara-C and hydroxyurea in three different cell types
title_full_unstemmed Detection of excision repaired DNA damage in the comet assay by using Ara-C and hydroxyurea in three different cell types
title_sort detection of excision repaired dna damage in the comet assay by using ara-c and hydroxyurea in three different cell types
publishDate 2009
url http://sedici.unlp.edu.ar/handle/10915/140052
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AT marcosricard detectionofexcisionrepaireddnadamageinthecometassaybyusingaracandhydroxyureainthreedifferentcelltypes
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