Detection of excision repaired DNA damage in the comet assay by using Ara-C and hydroxyurea in three different cell types
Because of its characteristics, the comet assay has been used to evaluate the ability of virtually any type of eukaryotic cell to repair different kinds of DNA damage, including double and single strand breaks and base damage. The ability to detect excision repair sites using the alkaline version ca...
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Formato: | Articulo |
Lenguaje: | Inglés |
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2009
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Acceso en línea: | http://sedici.unlp.edu.ar/handle/10915/140052 |
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I19-R120-10915-140052 |
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institution |
Universidad Nacional de La Plata |
institution_str |
I-19 |
repository_str |
R-120 |
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SEDICI (UNLP) |
language |
Inglés |
topic |
Ciencias Naturales Biología Comet assay Ara-C Hydroxyurea Alkylating agents Human cell lines |
spellingShingle |
Ciencias Naturales Biología Comet assay Ara-C Hydroxyurea Alkylating agents Human cell lines Güerci, Alba Mabel Liviac, D. Marcos, Ricard Detection of excision repaired DNA damage in the comet assay by using Ara-C and hydroxyurea in three different cell types |
topic_facet |
Ciencias Naturales Biología Comet assay Ara-C Hydroxyurea Alkylating agents Human cell lines |
description |
Because of its characteristics, the comet assay has been used to evaluate the ability of virtually any type of eukaryotic cell to repair different kinds of DNA damage, including double and single strand breaks and base damage. The ability to detect excision repair sites using the alkaline version can be enhanced by the inclusion of repair inhibitors, DNA synthesis inhibitors, or chain terminators. In this sense, we evaluated the ability of hydroxyurea (HU) and cytosine arabinoside (Ara-C), for detecting lesions produced by the alkylating agents ethyl methanesulfonate (EMS) and methyl methanesulfonate (MMS) in three different cell systems. Two hundred cells for experimental point were analyzed in the alkaline version of the comet assay, and the results are evidences of the utility of the assay to detect alkylation of bases in the cells lines MRC-5 and TK-6, as the treatment with HU +Ara-C significantly increases both the basal and induced frequency of DNA damage. The use of whole blood, although it detected the effects of MMS, with and without repair inhibitors, failed to detect the effect of the selected dose of EMS and does not permit detection increases in the background level. |
format |
Articulo Articulo |
author |
Güerci, Alba Mabel Liviac, D. Marcos, Ricard |
author_facet |
Güerci, Alba Mabel Liviac, D. Marcos, Ricard |
author_sort |
Güerci, Alba Mabel |
title |
Detection of excision repaired DNA damage in the comet assay by using Ara-C and hydroxyurea in three different cell types |
title_short |
Detection of excision repaired DNA damage in the comet assay by using Ara-C and hydroxyurea in three different cell types |
title_full |
Detection of excision repaired DNA damage in the comet assay by using Ara-C and hydroxyurea in three different cell types |
title_fullStr |
Detection of excision repaired DNA damage in the comet assay by using Ara-C and hydroxyurea in three different cell types |
title_full_unstemmed |
Detection of excision repaired DNA damage in the comet assay by using Ara-C and hydroxyurea in three different cell types |
title_sort |
detection of excision repaired dna damage in the comet assay by using ara-c and hydroxyurea in three different cell types |
publishDate |
2009 |
url |
http://sedici.unlp.edu.ar/handle/10915/140052 |
work_keys_str_mv |
AT guercialbamabel detectionofexcisionrepaireddnadamageinthecometassaybyusingaracandhydroxyureainthreedifferentcelltypes AT liviacd detectionofexcisionrepaireddnadamageinthecometassaybyusingaracandhydroxyureainthreedifferentcelltypes AT marcosricard detectionofexcisionrepaireddnadamageinthecometassaybyusingaracandhydroxyureainthreedifferentcelltypes |
bdutipo_str |
Repositorios |
_version_ |
1764820458222387200 |