Rhizobium biomass production in batch and continuous culture with a malt-sprouts medium
The growth of nine strains of four different species of Rhizobium (R. leguminosarum, R. meliloti, R. phaseoli and R. japonicum) was studied with media containing malt sprouts extract (MSE), in place of yeast extract (YE), as source of nitrogen and growth factors. The results obtained in batch cultur...
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| Autores principales: | , |
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| Formato: | Articulo |
| Lenguaje: | Inglés |
| Publicado: |
1985
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| Materias: | |
| Acceso en línea: | http://sedici.unlp.edu.ar/handle/10915/139617 |
| Aporte de: |
| Sumario: | The growth of nine strains of four different species of Rhizobium (R. leguminosarum, R. meliloti, R. phaseoli and R. japonicum) was studied with media containing malt sprouts extract (MSE), in place of yeast extract (YE), as source of nitrogen and growth factors. The results obtained in batch cultures indicated that all the strains grew well in MSE medium. In the case of fast-growing strains the biomass increased with increase in the concentration of MSE in the medium, but with the slow-growing strains, like R. japonicum, growth was inhibited completely by using a concentration of MSE (containing 3.75% w/v of solids) of 40% v/v. With all the strains the concentration of cells attained was greater than 5×109 cells/ml, which clearly indicates that MSE is a suitable component of media, and one which can be used instead of YE. The results of continuous culture experiments showed that a high productivity of cells (15.3×108 cells/ml/h) could be obtained using MSE medium. |
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