Quantification of protopectinase SE, an endopolygalacturonase with pectin-releasing activity from Geotrichum klebahnii

Pectin releasing activity of protopectinase SE (PPase-SE) from Geotrichum klebahnii (= G. penicillatum = Trichosporon penicillatum) ATCC 42397 was determined using different batches of lemon protopectin as substrate. Results obtained showed a high degree of variability depending on the batch of prot...

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Guardado en:
Detalles Bibliográficos
Autores principales: Cavalitto, Sebastián Fernando, Hours, Roque Alberto, Mignone, Carlos Fernando
Formato: Articulo
Lenguaje:Inglés
Publicado: 1999
Materias:
Ciencias Exactas
Física
endopolygalacturonases
Geotrichum klebahnii
pectin
pectin-releasing enzymes
protopectinases
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/138784
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
Descripción
Sumario:Pectin releasing activity of protopectinase SE (PPase-SE) from Geotrichum klebahnii (= G. penicillatum = Trichosporon penicillatum) ATCC 42397 was determined using different batches of lemon protopectin as substrate. Results obtained showed a high degree of variability depending on the batch of protopectin used. As PPase-SE also shows polygalacturonase (PGase) activity, a method for the assay of this activity was optimised. The best assay conditions were: substrate (polygalacturonic acid) concentration of 2.0 g 1−1, reaction time of 10 min and up to 0.17 PGase units per test tube.

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