Molecular data reveal hidden diversity of the genus <i>Clinostomum</i> (Digenea, Clinostomidae) in Argentina, with the description of a new species from <i>Ardea cocoi</i> (Ardeidae)

The genus <i>Clinostomum</i> has been recently a subject of a large number of molecular phylogenetic studies that have uncovered a larger species diversity than we thought. In Argentina, only two nominal species have been reported, namely <i>C. detruncatum</i> and <i>C....

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Autores principales: Montes, Martín Miguel, Barneche, Jorge Adrián, Pagano, Luis Gerardo, Ferrari, Walter Antonio Oscar, Martorelli, Sergio Roberto, Pérez Ponce de León, Gerardo
Formato: Articulo
Lenguaje:Inglés
Publicado: 2021
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Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/130578
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Sumario:The genus <i>Clinostomum</i> has been recently a subject of a large number of molecular phylogenetic studies that have uncovered a larger species diversity than we thought. In Argentina, only two nominal species have been reported, namely <i>C. detruncatum</i> and <i>C. marginatum</i>. Three putative species represented by metacercariae were recently molecularly diagnosed, and there are at least two additional metacercarial morphotypes diagnosed on morphological grounds. Here, we molecularly characterized specimens of <i>Clinostomum</i> sampled from freshwater fishes and fish-eating birds from Argentina through mtDNA cytochrome c oxidase subunit I (COI). Unexpectedly, the phylogenetic analysis uncovered three new additional genetic lineages, two of them corresponding to metacercarial stages and another matching COI sequences of <i>C. heluans</i> Braun, 1899, being considered conspecific, whereas the others still require formal description. Additionally, we add a new host species for a lineage molecularly diagnosed in a previous study. The adult specimens recovered from <i>A. cocoi</i> in Buenos Aires Province represented a new species clearly distinguished from the two species previously reported in Argentina. <i>Clinostomum detruncatum</i> is distinguished from the new species by possessing a characteristic tenoidean uterus, and testes located more posteriorly. <i>Clinostomum marginatum</i> is morphologically similar but differs from the new species in having rounded ovary, posterior testis lobated, and lateral cirrus-sac and displacing the anterior testis, and vitelline follicles not extending beyond the caeca end. Our study raises the number of <i>Clinostomum</i> species in Argentina up to 10. We describe the new species herein.