Spatiotemporal control of cell cycle acceleration during axolotl spinal cord regeneration
Axolotls are uniquely able to resolve spinal cord injuries, but little is known about the mechanisms underlying spinal cord regeneration. We previously found that tail amputation leads to reactivation of a developmental-like program in spinal cord ependymal cells (Rodrigo Albors et al., 2015), chara...
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I19-R120-10915-1299912023-04-26T12:49:51Z http://sedici.unlp.edu.ar/handle/10915/129991 Spatiotemporal control of cell cycle acceleration during axolotl spinal cord regeneration Cura Costa, Emanuel Otsuki, Leo Rodrigo Albors, Aida Tanaka, Elly M. Chara, Osvaldo 2021-12-23T17:44:04Z 2021-02-23 en Biología https://purl.org/becyt/ford/1.6 Axolotl Cell cycle Regeneration FUCCI Spinal cord Axolotls are uniquely able to resolve spinal cord injuries, but little is known about the mechanisms underlying spinal cord regeneration. We previously found that tail amputation leads to reactivation of a developmental-like program in spinal cord ependymal cells (Rodrigo Albors et al., 2015), characterized by a high-proliferation zone emerging 4 days post-amputation (Rost et al., 2016). What underlies this spatiotemporal pattern of cell proliferation, however, remained unknown. Here, we use modeling, tightly linked to experimental data, to demonstrate that this regenerative response is consistent with a signal that recruits ependymal cells during ~85 hours after amputation within ~830 μm of the injury. We adapted Fluorescent Ubiquitination-based Cell Cycle Indicator (FUCCI) technology to axolotls (AxFUCCI) to visualize cell cycles in vivo. AxFUCCI axolotls confirmed the predicted appearance time and size of the injury-induced recruitment zone and revealed cell cycle synchrony between ependymal cells. Our modeling and imaging move us closer to understanding bona fide spinal cord regeneration. This is the second release of the Jupyter Notebooks that contains source code for data analysis performed for Cura Costa et al., 2021. You can view the current version of the notebooks on GitHub or browse them online using nbviewer. Fil: Chara, Osvaldo. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Física de Líquidos y Sistemas Biológicos; Argentina. Technische Universität Dresden. Center for Information Services and High Performance Computing; Alemania. Fil: Cura Costa, Emanuel. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Física de Líquidos y Sistemas Biológicos; Argentina. Instituto de Física de Líquidos y Sistemas Biológicos Conjunto de datos Conjunto de datos http://creativecommons.org/licenses/by/4.0/ Creative Commons Attribution 4.0 International (CC BY 4.0) application/zip |
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Universidad Nacional de La Plata |
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R-120 |
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SEDICI (UNLP) |
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Inglés |
topic |
Biología https://purl.org/becyt/ford/1.6 Axolotl Cell cycle Regeneration FUCCI Spinal cord |
spellingShingle |
Biología https://purl.org/becyt/ford/1.6 Axolotl Cell cycle Regeneration FUCCI Spinal cord Cura Costa, Emanuel Otsuki, Leo Rodrigo Albors, Aida Tanaka, Elly M. Chara, Osvaldo Spatiotemporal control of cell cycle acceleration during axolotl spinal cord regeneration |
topic_facet |
Biología https://purl.org/becyt/ford/1.6 Axolotl Cell cycle Regeneration FUCCI Spinal cord |
description |
Axolotls are uniquely able to resolve spinal cord injuries, but little is known about the mechanisms underlying spinal cord regeneration. We previously found that tail amputation leads to reactivation of a developmental-like program in spinal cord ependymal cells (Rodrigo Albors et al., 2015), characterized by a high-proliferation zone emerging 4 days post-amputation (Rost et al., 2016). What underlies this spatiotemporal pattern of cell proliferation, however, remained unknown.
Here, we use modeling, tightly linked to experimental data, to demonstrate that this regenerative response is consistent with a signal that recruits ependymal cells during ~85 hours after amputation within ~830 μm of the injury. We adapted Fluorescent Ubiquitination-based Cell Cycle Indicator (FUCCI) technology to axolotls (AxFUCCI) to visualize cell cycles in vivo. AxFUCCI axolotls confirmed the predicted appearance time and size of the injury-induced recruitment zone and revealed cell cycle synchrony between ependymal cells. Our modeling and imaging move us closer to understanding bona fide spinal cord regeneration. |
format |
Conjunto de datos Conjunto de datos |
author |
Cura Costa, Emanuel Otsuki, Leo Rodrigo Albors, Aida Tanaka, Elly M. Chara, Osvaldo |
author_facet |
Cura Costa, Emanuel Otsuki, Leo Rodrigo Albors, Aida Tanaka, Elly M. Chara, Osvaldo |
author_sort |
Cura Costa, Emanuel |
title |
Spatiotemporal control of cell cycle acceleration during axolotl spinal cord regeneration |
title_short |
Spatiotemporal control of cell cycle acceleration during axolotl spinal cord regeneration |
title_full |
Spatiotemporal control of cell cycle acceleration during axolotl spinal cord regeneration |
title_fullStr |
Spatiotemporal control of cell cycle acceleration during axolotl spinal cord regeneration |
title_full_unstemmed |
Spatiotemporal control of cell cycle acceleration during axolotl spinal cord regeneration |
title_sort |
spatiotemporal control of cell cycle acceleration during axolotl spinal cord regeneration |
publishDate |
2021 |
url |
http://sedici.unlp.edu.ar/handle/10915/129991 |
work_keys_str_mv |
AT curacostaemanuel spatiotemporalcontrolofcellcycleaccelerationduringaxolotlspinalcordregeneration AT otsukileo spatiotemporalcontrolofcellcycleaccelerationduringaxolotlspinalcordregeneration AT rodrigoalborsaida spatiotemporalcontrolofcellcycleaccelerationduringaxolotlspinalcordregeneration AT tanakaellym spatiotemporalcontrolofcellcycleaccelerationduringaxolotlspinalcordregeneration AT charaosvaldo spatiotemporalcontrolofcellcycleaccelerationduringaxolotlspinalcordregeneration |
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1765659845560631296 |