Higher constitutive IL15Rα expression and lower IL‐15 response threshold in coeliac disease patients

The IL-15 triggering effect of gliadin is not exclusive to coeliac disease (CD) patients, whereas the secondary response is CD specific. We have studied the expression of the IL-15 receptor, and the IL-15 response upon stimulation, in non-CD and CD patients, and the possible existence of a lower imm...

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Autores principales: Bernardo, David, Garrote, José Antonio, Allegretti, Yessica Lorena, Leon, Alberto J., Gómez, E., Bermejo-Martin, Jesus F., Calvo, C., Riestra, Sabino, Fernández-Salazar, Luis, Blanco-Quirós, Alfredo, Chirdo, Fernando Gabriel, Arranz, Eduardo
Formato: Articulo
Lenguaje:Inglés
Publicado: 2008
Materias:
Medicina
Ciencias Exactas
Biología
Coeliac disease
IL-15
IL15R
immune threshold
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/127321
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-127321
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Medicina
Ciencias Exactas
Biología
Coeliac disease
IL-15
IL15R
immune threshold
spellingShingle Medicina
Ciencias Exactas
Biología
Coeliac disease
IL-15
IL15R
immune threshold
Bernardo, David
Garrote, José Antonio
Allegretti, Yessica Lorena
Leon, Alberto J.
Gómez, E.
Bermejo-Martin, Jesus F.
Calvo, C.
Riestra, Sabino
Fernández-Salazar, Luis
Blanco-Quirós, Alfredo
Chirdo, Fernando Gabriel
Arranz, Eduardo
Higher constitutive IL15Rα expression and lower IL‐15 response threshold in coeliac disease patients
topic_facet Medicina
Ciencias Exactas
Biología
Coeliac disease
IL-15
IL15R
immune threshold
description The IL-15 triggering effect of gliadin is not exclusive to coeliac disease (CD) patients, whereas the secondary response is CD specific. We have studied the expression of the IL-15 receptor, and the IL-15 response upon stimulation, in non-CD and CD patients, and the possible existence of a lower immunological threshold in the latter. Forty-two CD patients (20 on a gluten-containing diet, GCD, and 22 on gluten-free diet, GFD) and 24 non-CD healthy individuals were studied. IL15Rα mRNA expression, and tissue characterization, were assayed in the duodenum. Biopsies from six CD patients on GFD and 10 non-CD individuals were studied <i>in vitro</i> using organ culture in basal conditions, as well as after IL-15 stimulation discarding basal IL-15 production. Secretion of immune mediators was measured in the culture supernatants. IL15Rα mRNA expression was increased in CD patients, as compared with non-CD controls (on GFD P = 0·0334, on GCD P = 0·0062, respectively), and confirmed also by immunofluorescence. No differences were found between CD patients on GFD and on GCD. After <i>in vitro</i> IL-15 stimulation, IL15Rα expression was only triggered in non-CD controls (P = 0·0313), though it remained increased in CD patients. Moreover, IL-15 induced a more intense immunological response in CD patients after triggering the production of both nitrites and IFNγ (P = 0·0313, P = 0·0313, respectively). Gliadin-induced IL15 has a lower response threshold in CD patients, leading to the production of other immune mediators and the development of the intestinal lesion, and thus magnifying its effects within the CD intestine.
format Articulo
Articulo
author Bernardo, David
Garrote, José Antonio
Allegretti, Yessica Lorena
Leon, Alberto J.
Gómez, E.
Bermejo-Martin, Jesus F.
Calvo, C.
Riestra, Sabino
Fernández-Salazar, Luis
Blanco-Quirós, Alfredo
Chirdo, Fernando Gabriel
Arranz, Eduardo
author_facet Bernardo, David
Garrote, José Antonio
Allegretti, Yessica Lorena
Leon, Alberto J.
Gómez, E.
Bermejo-Martin, Jesus F.
Calvo, C.
Riestra, Sabino
Fernández-Salazar, Luis
Blanco-Quirós, Alfredo
Chirdo, Fernando Gabriel
Arranz, Eduardo
author_sort Bernardo, David
title Higher constitutive IL15Rα expression and lower IL‐15 response threshold in coeliac disease patients
title_short Higher constitutive IL15Rα expression and lower IL‐15 response threshold in coeliac disease patients
title_full Higher constitutive IL15Rα expression and lower IL‐15 response threshold in coeliac disease patients
title_fullStr Higher constitutive IL15Rα expression and lower IL‐15 response threshold in coeliac disease patients
title_full_unstemmed Higher constitutive IL15Rα expression and lower IL‐15 response threshold in coeliac disease patients
title_sort higher constitutive il15rα expression and lower il‐15 response threshold in coeliac disease patients
publishDate 2008
url http://sedici.unlp.edu.ar/handle/10915/127321
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