Thermodynamics of myoplasmic Ca²⁺ alternans reveal the molecular mechanisms involved in its genesis
Myoplasmic Ca²⁺ alternans commonly occur during conditions such as tachycardia, ischemia, or hypothermia. This is a serious condition that can lead to sudden cardiac death. Myoplasmic Ca²⁺ alternans are alternating beat-to-beat changes in the amplitude of the Ca²⁺ transient. They typically arise fro...
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| Autores principales: | , , |
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| Formato: | Articulo |
| Lenguaje: | Inglés |
| Publicado: |
2020
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| Materias: | |
| Acceso en línea: | http://sedici.unlp.edu.ar/handle/10915/126228 |
| Aporte de: |
| Sumario: | Myoplasmic Ca²⁺ alternans commonly occur during conditions such as tachycardia, ischemia, or hypothermia. This is a serious condition that can lead to sudden cardiac death. Myoplasmic Ca²⁺ alternans are alternating beat-to-beat changes in the amplitude of the Ca²⁺ transient. They typically arise from a variation in the amount of Ca²⁺ released from the sarcoplasmic reticulum (SR) between two consecutive heartbeats. This variability in the release of Ca²⁺ has previously been attributed to a delay in the recovery of the ryanodine receptor (RyR2), an incomplete Ca²⁺ refilling of the SR, or a change in the duration of the action potential. In each case, the RyR2 will mobilize Ca²⁺ from the SR in an alternating manner, thus generating Ca²⁺ alternans. To investigate the myoplasmic Ca²⁺ alternans in more depth, we utilized a novel experimental approach, Fluorescence Local Field Optical Mapping (FLOM), to record at the epicardial layer of an intact heart with subcellular resolution. These recordings were collected in conjunction with a local cold finger, where a temperature gradient was locally imposed on the tissue. In the colder regions, Ca²⁺ alternans were larger and occurred without changes in the duration of the action potential duration. Upon analyzing changes in the Q10 of several kinetic processes defining the intracellular Ca²⁺ dynamics, we found the imposed temperature gradient to have a significant effect on the relaxation of intracellular Ca²⁺ transients. The precipitous temperature dependency of Ca²⁺ alternans observed suggests they arise from an insufficient Ca²⁺ uptake into the SR by the ATPase of SR (SERCA2a). Interestingly, we found Ca2+ alternans to be heavily dependent on the SR Ca²⁺ and could be fostered with increased heart rate, which decreased the time for SERCA2a reuptake into SR beat to beat. Similarly, the partial pharmacological inhibition of SERCA2a with Thapsigargin increased the amplitude of myoplasmic Ca²⁺ alternans. Finally, the FLOM experimental approach is a valuable technique that can shed light on how arrhythmogenesis correlates with the spatial distribution of metabolically impaired myocytes along the myocardium. |
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