Identification of early stage recurrence endometrial cancer biomarkers using bioinformatics tools

Endometrial cancer (EC) is the sixth most common cancer in women worldwide. Early diagnosis is critical in recurrent EC management. The present study aimed to identify biomarkers of EC early recurrence using a workflow that combined text and data mining databases (DisGeNET, Gene Expression Omnibus),...

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Detalles Bibliográficos
Autores principales: Besso, María José, Montivero, Luciana, Lacunza, Ezequiel, Argibay, María Cecilia, Abba, Martín Carlos, Furlong, Laura Inés, Colas, Eva, Gil Moreno, Antonio, Reventos, Jaume, Bello, Ricardo, Vazquez Levin, Mónica Hebe
Formato: Articulo
Lenguaje:Inglés
Publicado: 2020
Materias:
Medicina
endometrial cancer
Bioinformatics
biomarkers
recurrence
TPX2
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/107852
http://europepmc.org/backend/ptpmcrender.fcgi?accid=PMC7388212&blobtype=pdf
https://www.spandidos-publications.com/10.3892/or.2020.7648
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
Descripción
Sumario:Endometrial cancer (EC) is the sixth most common cancer in women worldwide. Early diagnosis is critical in recurrent EC management. The present study aimed to identify biomarkers of EC early recurrence using a workflow that combined text and data mining databases (DisGeNET, Gene Expression Omnibus), a prioritization algorithm to select a set of putative candidates (ToppGene), protein-protein interaction network analyses (Search Tool for the Retrieval of Interacting Genes, cytoHubba), association analysis of selected genes with clinicopathological parameters, and survival analysis (Kaplan-Meier and Cox proportional hazard ratio analyses) using a The Cancer Genome Atlas cohort. A total of 10 genes were identified, among which the targeting protein for Xklp2 (<i>TPX2</i>) was the most promising independent prognostic biomarker in stage I EC. <i>TPX2</i> expression (mRNA and protein) was higher (P<0.0001 and P<0.001, respectively) in ETS variant transcription factor 5-overexpressing Hec1a and Ishikawa cells, a previously reported cell model of aggressive stage I EC. In EC biopsies, <i>TPX2</i> mRNA expression levels were higher (P<0.05) in high grade tumors (grade 3) compared with grade 1-2 tumors (P<0.05), in tumors with deep myometrial invasion (>50% compared with <50%; P<0.01), and in intermediate-high recurrence risk tumors compared with low-risk tumors (P<0.05). Further validation studies in larger and independent EC cohorts will contribute to confirm the prognostic value of <i>TPX2</i>.

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