Influence of individual cell motility on the 2D front roughness dynamics of tumour cell colonies

The dynamics of in situ 2D HeLa cell quasi-linear and quasi-radial colony fronts in a standard culture medium is investigated. For quasi-radial colonies, as the cell population increased, a kinetic transition from an exponential to a constant front average velocity regime was observed. Special atten...

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Autores principales: Muzzio, Nicolás Eduardo, Pasquale, Miguel Ángel, González, Pedro Horacio, Arvia, Alejandro Jorge
Formato: Articulo
Lenguaje:Español
Publicado: 2014
Materias:
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/107740
http://europepmc.org/backend/ptpmcrender.fcgi?accid=PMC4059834&blobtype=pdf
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id I19-R120-10915-107740
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Español
topic Ciencias Médicas
Ciencias Exactas
Cell population
Colony spatio-temporal heterogeneity
Crowding effects
Biased cell trajectories
Cell migration concerted mechanism
spellingShingle Ciencias Médicas
Ciencias Exactas
Cell population
Colony spatio-temporal heterogeneity
Crowding effects
Biased cell trajectories
Cell migration concerted mechanism
Muzzio, Nicolás Eduardo
Pasquale, Miguel Ángel
González, Pedro Horacio
Arvia, Alejandro Jorge
Influence of individual cell motility on the 2D front roughness dynamics of tumour cell colonies
topic_facet Ciencias Médicas
Ciencias Exactas
Cell population
Colony spatio-temporal heterogeneity
Crowding effects
Biased cell trajectories
Cell migration concerted mechanism
description The dynamics of in situ 2D HeLa cell quasi-linear and quasi-radial colony fronts in a standard culture medium is investigated. For quasi-radial colonies, as the cell population increased, a kinetic transition from an exponential to a constant front average velocity regime was observed. Special attention was paid to individual cell motility evolution under constant average colony front velocity looking for its impact on the dynamics of the 2D colony front roughness. From the directionalities and velocity components of cell trajectories in colonies with different cell populations, the influence of both local cell density and cell crowding effects on individual cell motility was determined. The average dynamic behaviour of individual cells in the colony and its dependence on both local spatio-temporal heterogeneities and growth geometry suggested that cell motion undergoes under a concerted cell migration mechanism, in which both a limiting random walk-like and a limiting ballistic-like contribution were involved. These results were interesting to infer how biased cell trajectories influenced both the 2D colony spreading dynamics and the front roughness characteristics by local biased contributions to individual cell motion. These data are consistent with previous experimental and theoretical cell colony spreading data and provide additional evidence of the validity of the Kardar-Parisi-Zhang equation, within a certain range of time and colony front size, for describing the dynamics of 2D colony front roughness.
format Articulo
Articulo
author Muzzio, Nicolás Eduardo
Pasquale, Miguel Ángel
González, Pedro Horacio
Arvia, Alejandro Jorge
author_facet Muzzio, Nicolás Eduardo
Pasquale, Miguel Ángel
González, Pedro Horacio
Arvia, Alejandro Jorge
author_sort Muzzio, Nicolás Eduardo
title Influence of individual cell motility on the 2D front roughness dynamics of tumour cell colonies
title_short Influence of individual cell motility on the 2D front roughness dynamics of tumour cell colonies
title_full Influence of individual cell motility on the 2D front roughness dynamics of tumour cell colonies
title_fullStr Influence of individual cell motility on the 2D front roughness dynamics of tumour cell colonies
title_full_unstemmed Influence of individual cell motility on the 2D front roughness dynamics of tumour cell colonies
title_sort influence of individual cell motility on the 2d front roughness dynamics of tumour cell colonies
publishDate 2014
url http://sedici.unlp.edu.ar/handle/10915/107740
http://europepmc.org/backend/ptpmcrender.fcgi?accid=PMC4059834&blobtype=pdf
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