A <i>Bordetella pertussis</i> MgtC homolog plays a role in the intracellular survival
<i>Bordetella pertussis</i>, the causative agent of whooping cough, has the capability to survive inside the host cells. This process requires efficient adaptation of the pathogen to the intracellular environment and the associated stress. Among the proteins produced by the intracellular...
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| Autores principales: | , , , , |
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| Formato: | Articulo |
| Lenguaje: | Inglés |
| Publicado: |
2018
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| Materias: | |
| Acceso en línea: | http://sedici.unlp.edu.ar/handle/10915/104721 http://hdl.handle.net/11336/93561 https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0203204 |
| Aporte de: |
| Sumario: | <i>Bordetella pertussis</i>, the causative agent of whooping cough, has the capability to survive inside the host cells. This process requires efficient adaptation of the pathogen to the intracellular environment and the associated stress. Among the proteins produced by the intracellular <i>B. pertussis</i> we identified a protein (BP0414) that shares homology with MgtC, a protein which was previously shown to be involved in the intracellular survival of other pathogens. To explore if BP0414 plays a role in <i>B. pertussis</i> intracellular survival a mutant strain defective in the production of this protein was constructed. Using standard <i>in vitro</i> growth conditions we found that BP0414 is required for <i>B. pertussis</i> growth under low magnesium availability or low pH, two environmental conditions that this pathogen might face within the host cell. Intracellular survival studies showed that MgtC is indeed involved in <i>B. pertussis</i> viability inside the macrophages. The use of bafilomycin A1, which inhibits phagosome acidification, abolished the survival defect of the <i>mgtC</i> deficient mutant strain suggesting that in intracellular <i>B. pertussis</i> the role of MgtC protein is mainly related to the bacterial adaptation to the acidic conditions found inside the of phagosomes. Overall, this work provides an insight into the importance of MgtC in <i>B. pertussis</i> pathogenesis and its contribution to bacterial survival within immune cells. |
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