Relationship between genotoxic effects of breast cancer treatments and patient basal DNA integrity

It is accepted that radiotherapy and chemotherapy cause genotoxic effects as part of their therapy side effects, which are highly variable among different patients. This study evaluated DNA integrity using the comet assay in peripheral blood lymphocytes from breast cancer patients before (“pre-treat...

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Autores principales: Capitaine Funes, Juan, Massa, Estefania, Cipulli, Germán, Benitez Gil, Alfonso, Capitaine Funes, Carlos, Tozzini, Roberto, Ghersevich, Sergio, Ceballos, María Paula
Formato: article artículo acceptedVersion
Lenguaje:Inglés
Publicado: BEGELL HOUSE INC 2017
Materias:
Acceso en línea:http://hdl.handle.net/2133/9378
http://hdl.handle.net/2133/9378
Aporte de:Repositorio Hipermedial de la Universidad Nacional de Rosario (UNR) de Universidad Nacional de Rosario Ver origen
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spelling I15-R121-2133-93782017-11-23T18:44:12Z Relationship between genotoxic effects of breast cancer treatments and patient basal DNA integrity Capitaine Funes, Juan Massa, Estefania Cipulli, Germán Benitez Gil, Alfonso Capitaine Funes, Carlos Tozzini, Roberto Ghersevich, Sergio Ceballos, María Paula Ensayo cometa Daño genotóxico Cáncer mamas Factor pronóstico Peripheral blood lymphocytes Comet assay Genotoxic damage It is accepted that radiotherapy and chemotherapy cause genotoxic effects as part of their therapy side effects, which are highly variable among different patients. This study evaluated DNA integrity using the comet assay in peripheral blood lymphocytes from breast cancer patients before (“pre-treatment patients”; n = 47) and after (“post-treatment patients”; n = 24) radiotherapy and/or chemotherapy treatment and from healthy donors (n = 15). Comet evaluation was made by visual (types 0-4) and digital (percentage of DNA remaining in the comet head = % head DNA) analysis. The association between the level of DNA damage and cancer prognostic factors was assessed. The treatments caused a significant increase in DNA damage registered by both visual (p < 0.001) and digital (p < 0.001) analysis. No significant associations between the level of DNA damage in pre-treatment patients and cancer prognostic factors were found. Results showed a significant correlation between the comet results from each patient before and after treatment (r = 0.64, p = 0.001). The % head DNA in post-treatment samples from patients with high level of DNA damage before treatment (30.3 ± 3.1%, p < 0.01) was lower than in post-treatment samples from patient with low to medium level of DNA damage before therapy (49.2 ± 4.4%). Results support the usefulness of the comet assay as a sensitive technique to evaluate the basal DNA status and the damage caused by cancer treatments. The assay could contribute to the treatment decisions, taking into account the patients’ basal DNA damage before therapy. Fil: Capitaine Funes, Juan. Universidad Nacional de Rosario. Facultad de Ciencias Médicas; Argentina Fil: Ceballos, María Paula. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Fisiología Experimental (UNR-CONICET); Argentina Fil: Capitaine Funes, Juan. Provincia de Santa Fe. Hospital Provincial del Centenario. Servicio de Mastología; Argentina Fil: Massa, Estefania. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina Fil: Cipulli, Germán. Provincia de Santa Fe. Hospital Provincial del Centenario. Servicio de Mastología; Argentina Fil: Benitez Gil, Alfonso. Provincia de Santa Fe. Hospital Provincial. Servicio de Mastología; Argentina Fil: Capitaine Funes, Carlos. Universidad Nacional de Rosario. Facultad de Ciencias Médicas; Argentina Fil: Capitaine Funes, Carlos. Provincia de Santa Fe. Hospital Provincial del Centenario. Servicio de Mastología; Argentina Fil: Tozzini, Roberto. Universidad Nacional de Rosario. Facultad de Ciencias Médicas; Argentina Fil: Tozzini, Roberto. Provincia de Santa Fe. Hospital Provincial del Centenario. Servicio de Mastología; Argentina Fil: Ghersevich, Sergio. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina 2017-11-23T18:41:19Z 2017-11-23T18:41:19Z 2014-04-15 2017-11-23T18:41:19Z 2017-11-23T18:41:19Z 2014-04-15 article artículo acceptedVersion 0731-8898 http://hdl.handle.net/2133/9378 http://hdl.handle.net/2133/9378 eng http://www.dl.begellhouse.com/journals/0ff459a57a4c08d0,249005dc454eb8de,570be1034750e71e.html DOI: 10.1615/JEnvironPatholToxicolOncol.2014010592 Begell House Inc. Ceballos, María Paula Capitaine Funes, Juan Massa, Estefanía Cipulli, Germán Benitez Gil, Alfonso Capitaine Funes, Carlos Tozzini, Roberto Ghersevich, Sergio openAccess application/pdf BEGELL HOUSE INC
institution Universidad Nacional de Rosario
institution_str I-15
repository_str R-121
collection Repositorio Hipermedial de la Universidad Nacional de Rosario (UNR)
language Inglés
orig_language_str_mv eng
topic Ensayo cometa
Daño genotóxico
Cáncer mamas
Factor pronóstico
Peripheral blood lymphocytes
Comet assay
Genotoxic damage
spellingShingle Ensayo cometa
Daño genotóxico
Cáncer mamas
Factor pronóstico
Peripheral blood lymphocytes
Comet assay
Genotoxic damage
Capitaine Funes, Juan
Massa, Estefania
Cipulli, Germán
Benitez Gil, Alfonso
Capitaine Funes, Carlos
Tozzini, Roberto
Ghersevich, Sergio
Ceballos, María Paula
Relationship between genotoxic effects of breast cancer treatments and patient basal DNA integrity
topic_facet Ensayo cometa
Daño genotóxico
Cáncer mamas
Factor pronóstico
Peripheral blood lymphocytes
Comet assay
Genotoxic damage
description It is accepted that radiotherapy and chemotherapy cause genotoxic effects as part of their therapy side effects, which are highly variable among different patients. This study evaluated DNA integrity using the comet assay in peripheral blood lymphocytes from breast cancer patients before (“pre-treatment patients”; n = 47) and after (“post-treatment patients”; n = 24) radiotherapy and/or chemotherapy treatment and from healthy donors (n = 15). Comet evaluation was made by visual (types 0-4) and digital (percentage of DNA remaining in the comet head = % head DNA) analysis. The association between the level of DNA damage and cancer prognostic factors was assessed. The treatments caused a significant increase in DNA damage registered by both visual (p < 0.001) and digital (p < 0.001) analysis. No significant associations between the level of DNA damage in pre-treatment patients and cancer prognostic factors were found. Results showed a significant correlation between the comet results from each patient before and after treatment (r = 0.64, p = 0.001). The % head DNA in post-treatment samples from patients with high level of DNA damage before treatment (30.3 ± 3.1%, p < 0.01) was lower than in post-treatment samples from patient with low to medium level of DNA damage before therapy (49.2 ± 4.4%). Results support the usefulness of the comet assay as a sensitive technique to evaluate the basal DNA status and the damage caused by cancer treatments. The assay could contribute to the treatment decisions, taking into account the patients’ basal DNA damage before therapy.
format article
artículo
acceptedVersion
author Capitaine Funes, Juan
Massa, Estefania
Cipulli, Germán
Benitez Gil, Alfonso
Capitaine Funes, Carlos
Tozzini, Roberto
Ghersevich, Sergio
Ceballos, María Paula
author_facet Capitaine Funes, Juan
Massa, Estefania
Cipulli, Germán
Benitez Gil, Alfonso
Capitaine Funes, Carlos
Tozzini, Roberto
Ghersevich, Sergio
Ceballos, María Paula
author_sort Capitaine Funes, Juan
title Relationship between genotoxic effects of breast cancer treatments and patient basal DNA integrity
title_short Relationship between genotoxic effects of breast cancer treatments and patient basal DNA integrity
title_full Relationship between genotoxic effects of breast cancer treatments and patient basal DNA integrity
title_fullStr Relationship between genotoxic effects of breast cancer treatments and patient basal DNA integrity
title_full_unstemmed Relationship between genotoxic effects of breast cancer treatments and patient basal DNA integrity
title_sort relationship between genotoxic effects of breast cancer treatments and patient basal dna integrity
publisher BEGELL HOUSE INC
publishDate 2017
url http://hdl.handle.net/2133/9378
http://hdl.handle.net/2133/9378
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