CuA-based chimeric T1 copper sites allow for independent modulation of reorganization energy and reduction potential
Attaining rational modulation of thermodynamic and kinetic redox parameters of metalloproteins is a key milestone towards the (re)design of proteins with new or improved redox functions. Here we report that implantation of ligand loops from natural T1 proteins into the scaffold of a CuA protein le...
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| Autores principales: | , , , , , , |
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| Formato: | article artículo publishedVersion |
| Lenguaje: | Inglés |
| Publicado: |
Royal Society of Chemistry
2021
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/2133/20481 http://hdl.handle.net/2133/20481 |
| Aporte de: |
| id |
I15-R121-2133-20481 |
|---|---|
| record_format |
dspace |
| institution |
Universidad Nacional de Rosario |
| institution_str |
I-15 |
| repository_str |
R-121 |
| collection |
Repositorio Hipermedial de la Universidad Nacional de Rosario (UNR) |
| language |
Inglés |
| topic |
Copper Metalloproteins Oxidation-Reduction Ligands |
| spellingShingle |
Copper Metalloproteins Oxidation-Reduction Ligands Szuster, Jonathan Zitare, Ulises A. Castro, María A. Leguto, Alcides J. Morgada, Marcos Nicolás Vila, Alejandro J. Murgida, Daniel H. CuA-based chimeric T1 copper sites allow for independent modulation of reorganization energy and reduction potential |
| topic_facet |
Copper Metalloproteins Oxidation-Reduction Ligands |
| description |
Attaining rational modulation of thermodynamic and kinetic redox parameters of metalloproteins is a key
milestone towards the (re)design of proteins with new or improved redox functions. Here we report that
implantation of ligand loops from natural T1 proteins into the scaffold of a CuA protein leads to a series
of distorted T1-like sites that allow for independent modulation of reduction potentials (E°´) and electron
transfer reorganization energies (l). On the one hand E°´ values could be fine-tuned over 120 mV
without affecting l. On the other, l values could be modulated by more than a factor of two while
affecting E°´ only by a few millivolts. These results are in sharp contrast to previous studies that used T1
cupredoxin folds, thus highlighting the importance of the protein scaffold in determining such parameters |
| format |
article artículo publishedVersion |
| author |
Szuster, Jonathan Zitare, Ulises A. Castro, María A. Leguto, Alcides J. Morgada, Marcos Nicolás Vila, Alejandro J. Murgida, Daniel H. |
| author_facet |
Szuster, Jonathan Zitare, Ulises A. Castro, María A. Leguto, Alcides J. Morgada, Marcos Nicolás Vila, Alejandro J. Murgida, Daniel H. |
| author_sort |
Szuster, Jonathan |
| title |
CuA-based chimeric T1 copper sites allow for
independent modulation of reorganization energy
and reduction potential |
| title_short |
CuA-based chimeric T1 copper sites allow for
independent modulation of reorganization energy
and reduction potential |
| title_full |
CuA-based chimeric T1 copper sites allow for
independent modulation of reorganization energy
and reduction potential |
| title_fullStr |
CuA-based chimeric T1 copper sites allow for
independent modulation of reorganization energy
and reduction potential |
| title_full_unstemmed |
CuA-based chimeric T1 copper sites allow for
independent modulation of reorganization energy
and reduction potential |
| title_sort |
cua-based chimeric t1 copper sites allow for
independent modulation of reorganization energy
and reduction potential |
| publisher |
Royal Society of Chemistry |
| publishDate |
2021 |
| url |
http://hdl.handle.net/2133/20481 http://hdl.handle.net/2133/20481 |
| work_keys_str_mv |
AT szusterjonathan cuabasedchimerict1coppersitesallowforindependentmodulationofreorganizationenergyandreductionpotential AT zitareulisesa cuabasedchimerict1coppersitesallowforindependentmodulationofreorganizationenergyandreductionpotential AT castromariaa cuabasedchimerict1coppersitesallowforindependentmodulationofreorganizationenergyandreductionpotential AT legutoalcidesj cuabasedchimerict1coppersitesallowforindependentmodulationofreorganizationenergyandreductionpotential AT morgadamarcosnicolas cuabasedchimerict1coppersitesallowforindependentmodulationofreorganizationenergyandreductionpotential AT vilaalejandroj cuabasedchimerict1coppersitesallowforindependentmodulationofreorganizationenergyandreductionpotential AT murgidadanielh cuabasedchimerict1coppersitesallowforindependentmodulationofreorganizationenergyandreductionpotential |
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Repositorios |
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