The Notch system during pubertal development of the bovine mammary gland

The development of the mammary gland of cows during pre-weaning and puberty will condition its future productive capacity and warrants special study. In this respect, Notch signaling regulates tissue development and fate by modifying cell proliferation and differentiation and has been involved in st...

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Autores principales: Bonadeo, Nadia, Becu-Villalobos, Damasia, Cristina, Carolina, Lacau-Mengido, Isabel M.
Otros Autores: https://orcid.org/0000-0002-5641-3109
Formato: Artículo publishedVersion
Lenguaje:Inglés
Publicado: Nature Research 2021
Materias:
Notch
Bovine mammary gland
Development
Acceso en línea:http://repositorio.unnoba.edu.ar:8080/xmlui/handle/23601/113
Aporte de:
Re DI Repositorio Digital UNNOBA de Universidad Nacional del Noroeste de la Provincia de Buenos Aires
id I103-R405-23601-113
record_format dspace
spelling I103-R405-23601-1132025-10-21T21:07:16Z The Notch system during pubertal development of the bovine mammary gland Bonadeo, Nadia Becu-Villalobos, Damasia Cristina, Carolina Lacau-Mengido, Isabel M. https://orcid.org/0000-0002-5641-3109 https://orcid.org/0000-0003-2547-3819 https://orcid.org/0000-0003-0459-6721 Notch Bovine mammary gland Development The development of the mammary gland of cows during pre-weaning and puberty will condition its future productive capacity and warrants special study. In this respect, Notch signaling regulates tissue development and fate by modifying cell proliferation and differentiation and has been involved in stem cell maintenance, but has not been extensively studied in the developing mammary glands in cows. We therefore investigated Notch receptor expression and localization, as well as the expression of Notch ligands and target genes in the mammary gland of Holstein heifers in pre- and post-pubertal stages. Notch receptors 1 to 4 were detected by immunohistochemistry in the parenchyma and stroma of the developing gland. The subcellular localization of the four receptors was predominantly cytoplasmic except for NOTCH4, which was mostly nuclear. The membrane and the active intracellular domains of NOTCH paralogues were identified by western blot. NOTCH1 and NOTCH2 active domains increased during pubertal stages while NOTCH3 and NOTCH4 active domains decreased, suggesting strikingly different involvement of NOTCH paralogues in bovine mammary gland development and differentiation. The mRNA expression levels of the target genes HEY1 and HEY2 increased during peri-puberty whereas no variation of HES1 mRNA levels was observed. The mRNA levels of the Notch ligands JAGGED1 and DELTA1 also increased gradually during development. In conclusion, Notch signaling system dynamically varies throughout the development of the mammary gland during puberty pointing to specific time involvement of each component. Fil: Bonadeo, Nadia. Universidad Nacional del Noroeste de la Provincia de Buenos Aires. CIT NOBA (UNNOBA-UNSADA-CONICET). Centro de Investigaciones Básicas y Aplicadas. Laboratorio de Fisiopatología de la Hipófisis; Argentina. Fil: Becu-Villalobos, Damasia. Universidad de Buenos Aires. Instituto de Biología y Medicina Experimental (IByME-CONICET). Laboratorio de Regulación Hipofisaria; Argentina. Fil: Cristina, Carolina. Universidad Nacional del Noroeste de la Provincia de Buenos Aires. CIT NOBA (UNNOBA-UNSADA-CONICET). Centro de Investigaciones Básicas y Aplicadas. Laboratorio de Fisiopatología de la Hipófisis; Argentina. Fil: Lacau-Mengido, Isabel M. Universidad de Buenos Aires. Instituto de Biología y Medicina Experimental (IByME-CONICET). Laboratorio de Regulación Hipofisaria; Argentina. Con referato 2021-02-03T16:10:36Z 2021-02-03T16:10:36Z 2019-06-20 info:eu-repo/semantics/article info:ar-repo/semantics/artículo info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article info:ar-repo/semantics/artículo info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article info:ar-repo/semantics/artículo info:eu-repo/semantics/publishedVersion Bonadeo N, Becu-Villalobos D, Cristina C, Lacau-Mengido IM. The Notch system during pubertal development of the bovine mammary gland. Sci Rep. 2019 Jun 20;9(1):8899. doi: 10.1038/s41598-019-45406-6. PMID: 31222104; PMCID: PMC6586787. 2045-2322 http://repositorio.unnoba.edu.ar:8080/xmlui/handle/23601/113 eng 10.1038/s41598-019-45406-6 info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-nd/2.5/ar/ application/pdf application/pdf Nature Research Scientific Reports
institution Universidad Nacional del Noroeste de la Provincia de Buenos Aires
institution_str I-103
repository_str R-405
collection Re DI Repositorio Digital UNNOBA
language Inglés
topic Notch
Bovine mammary gland
Development
spellingShingle Notch
Bovine mammary gland
Development
Bonadeo, Nadia
Becu-Villalobos, Damasia
Cristina, Carolina
Lacau-Mengido, Isabel M.
The Notch system during pubertal development of the bovine mammary gland
topic_facet Notch
Bovine mammary gland
Development
description The development of the mammary gland of cows during pre-weaning and puberty will condition its future productive capacity and warrants special study. In this respect, Notch signaling regulates tissue development and fate by modifying cell proliferation and differentiation and has been involved in stem cell maintenance, but has not been extensively studied in the developing mammary glands in cows. We therefore investigated Notch receptor expression and localization, as well as the expression of Notch ligands and target genes in the mammary gland of Holstein heifers in pre- and post-pubertal stages. Notch receptors 1 to 4 were detected by immunohistochemistry in the parenchyma and stroma of the developing gland. The subcellular localization of the four receptors was predominantly cytoplasmic except for NOTCH4, which was mostly nuclear. The membrane and the active intracellular domains of NOTCH paralogues were identified by western blot. NOTCH1 and NOTCH2 active domains increased during pubertal stages while NOTCH3 and NOTCH4 active domains decreased, suggesting strikingly different involvement of NOTCH paralogues in bovine mammary gland development and differentiation. The mRNA expression levels of the target genes HEY1 and HEY2 increased during peri-puberty whereas no variation of HES1 mRNA levels was observed. The mRNA levels of the Notch ligands JAGGED1 and DELTA1 also increased gradually during development. In conclusion, Notch signaling system dynamically varies throughout the development of the mammary gland during puberty pointing to specific time involvement of each component.
author2 https://orcid.org/0000-0002-5641-3109
author_facet https://orcid.org/0000-0002-5641-3109
Bonadeo, Nadia
Becu-Villalobos, Damasia
Cristina, Carolina
Lacau-Mengido, Isabel M.
format Artículo
Artículo
publishedVersion
Artículo
Artículo
publishedVersion
Artículo
Artículo
publishedVersion
author Bonadeo, Nadia
Becu-Villalobos, Damasia
Cristina, Carolina
Lacau-Mengido, Isabel M.
author_sort Bonadeo, Nadia
title The Notch system during pubertal development of the bovine mammary gland
title_short The Notch system during pubertal development of the bovine mammary gland
title_full The Notch system during pubertal development of the bovine mammary gland
title_fullStr The Notch system during pubertal development of the bovine mammary gland
title_full_unstemmed The Notch system during pubertal development of the bovine mammary gland
title_sort notch system during pubertal development of the bovine mammary gland
publisher Nature Research
publishDate 2021
url http://repositorio.unnoba.edu.ar:8080/xmlui/handle/23601/113
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