Microarray analysis, pre-processing. Quality in the selection of differentiatedly expressed genes
Following the success of microarray technology, in the literature there is a large number of experiments made with them. However, the problems of standardization and the many sources of variability make it necessary posteriori validation techniques. For this reason, we have tried to study how the se...
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| Autores principales: | , , |
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| Formato: | Artículo revista |
| Lenguaje: | Español |
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Escuela de Perfeccionamiento en Investigación Operativa
2018
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| Acceso en línea: | https://revistas.unc.edu.ar/index.php/epio/article/view/20345 |
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| Sumario: | Following the success of microarray technology, in the literature there is a large number of experiments made with them. However, the problems of standardization and the many sources of variability make it necessary posteriori validation techniques. For this reason, we have tried to study how the selection of genes influence some key preprocessing techniques. Many of the studies conducted to compare these techniques have been carried out on experiments which optimal results are known a priori to try to determine which has greater accuracy. In our case, we do not know the correct result a priori and what has been accomplished is a comparative analysis of the results obtained in each case in order to predict a priori the behavior of each of the techniques discussed in terms of various factors as initial data distribution, expression patterns object of interest, the presence of outliers, and so on.Three techniques have been applied on the preprocessing on a microarray experiment. The techniques are GCRMA, MAS5 and MBEI. In our data there have been found mainly three patterns of expression in those genes expressed and have been shown statistically that there is an association between the pre-processing technique used and the predominant pattern in it. This trend is related to efficiency in the detection of outliers and the magnitude of change detected with each of them. So far, it has not been able to establish a statistically significant in confirming the agreement between the three methods after the selection of differentially expressed genes. |
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