Quercetin as natural strategy to avoid plasma lipid imbalance and intestinal mucosa oxidative stress caused by high fructose diet

High Fructose Diet (HFD) is associated with development of metabolic syndrome, oxidative stress (OS) and inflammation. In recent decades, polyphenols have been postulated as strategic nutrients in antioxidant functional diets. Aim was to evaluate Quercetin (Q) ability to prevent OS in intestinal muc...

Descripción completa

Guardado en:
Detalles Bibliográficos
Autores principales: León, M, Lobo, V, Luciani, N, Salcedo, R, Moine, L, Díaz de Barboza, G
Formato: Artículo revista
Lenguaje:Español
Publicado: Universidad Nacional Córdoba. Facultad de Ciencias Médicas. Secretaria de Ciencia y Tecnología 2023
Materias:
High Fructose Diet
quercetin
oxidative stress
dyslipidemia
Dieta Alta en Fructosa
quercetina
estrés oxidativo
dislipemia
Acceso en línea:https://revistas.unc.edu.ar/index.php/med/article/view/42695
Aporte de:
Revista de la Facultad de Ciencias Médicas de Córdoba de Universidad Nacional de Córdoba
id I10-R327-article-42695
record_format ojs
institution Universidad Nacional de Córdoba
institution_str I-10
repository_str R-327
container_title_str Revista de la Facultad de Ciencias Médicas de Córdoba
language Español
format Artículo revista
topic High Fructose Diet
quercetin
oxidative stress
dyslipidemia
Dieta Alta en Fructosa
quercetina
estrés oxidativo
dislipemia
spellingShingle High Fructose Diet
quercetin
oxidative stress
dyslipidemia
Dieta Alta en Fructosa
quercetina
estrés oxidativo
dislipemia
León, M
Lobo, V
Luciani, N
Salcedo, R
Moine, L
Díaz de Barboza, G
Quercetin as natural strategy to avoid plasma lipid imbalance and intestinal mucosa oxidative stress caused by high fructose diet
topic_facet High Fructose Diet
quercetin
oxidative stress
dyslipidemia
Dieta Alta en Fructosa
quercetina
estrés oxidativo
dislipemia
author León, M
Lobo, V
Luciani, N
Salcedo, R
Moine, L
Díaz de Barboza, G
author_facet León, M
Lobo, V
Luciani, N
Salcedo, R
Moine, L
Díaz de Barboza, G
author_sort León, M
title Quercetin as natural strategy to avoid plasma lipid imbalance and intestinal mucosa oxidative stress caused by high fructose diet
title_short Quercetin as natural strategy to avoid plasma lipid imbalance and intestinal mucosa oxidative stress caused by high fructose diet
title_full Quercetin as natural strategy to avoid plasma lipid imbalance and intestinal mucosa oxidative stress caused by high fructose diet
title_fullStr Quercetin as natural strategy to avoid plasma lipid imbalance and intestinal mucosa oxidative stress caused by high fructose diet
title_full_unstemmed Quercetin as natural strategy to avoid plasma lipid imbalance and intestinal mucosa oxidative stress caused by high fructose diet
title_sort quercetin as natural strategy to avoid plasma lipid imbalance and intestinal mucosa oxidative stress caused by high fructose diet
description High Fructose Diet (HFD) is associated with development of metabolic syndrome, oxidative stress (OS) and inflammation. In recent decades, polyphenols have been postulated as strategic nutrients in antioxidant functional diets. Aim was to evaluate Quercetin (Q) ability to prevent OS in intestinal mucosa and to avoid glycemia and lipemia alterations generated by HFD. Wistar rats were divided into different study groups: Control (C, no treatment); subjected to HFD for 60 days (HFD) and rats treated with HFD and Q (25, 50 or 75 mg/kg/pc, HFD+Q) last 15 days of treatment. After animal’s sacrifice, glucose, HbA1c, triglycerides (TG), total cholesterol and HDL-cholesterol in blood and glutathione (GSH) content and superoxide dismutase (SOD) and catalase (CAT) activity in intestinal mucosa homogenate were determined. Results were analyzed by ANOVA/Tukey (p<0.05). All groups presented similar values of glycemia, HbA1c and total cholesterol. HFD rats had high TG levels and decreased HDL-cholesterol levels in relation to C (185±9.75 HFD* vs 90.75±7.26 C, *p<0.001 and 20±1.08 HFD* vs 26.25±1.65 C, *p<0.001), which was reflected in TG/HDL-col ratio increased, a cardiovascular risk and insulin resistance indicator (9.30±0, 57 HFD* vs 3.48±0.26 C, *p<0.001). Q evidences its protective effect by normalizing values of these parameters (TG/HDL-cholesterol: 7.29±0.42 HFD+Q25; 4.55±0.35 HFD+Q50; 5.60±0.30 HFD+Q75, *p<0.001 vs HFD). In relation to OS, we found that Q prevents SOD activity increment (31.22±3.5 HFD* vs 4.26±2.91 C; 9.85±4.03 HFD+Q25; 11.81±4.7 HFD+Q50, 17.59±3 HFD+Q75, *p<0.05) and CAT (45.85±5.26 HFD* vs 11.55±4.88 C, 17.34 ±3.98 HFD+Q25, 28.54±3.04 HFD+Q50, 17.67±3.89 HFD+Q75, *p<0.001). Added to this effect Q had ability to prevent GSH decrease in rat intestinal mucosa (3.93±0.14 HFD* vs 6.99±0.64 C, 7.22±0.55 HFD+ Q25 and 9.35±1.12 HFD+Q50, *p<0.05). Our results indicate that Q has a protective effect against dyslipidemia and OS in intestinal mucosa generated by high fructose consumption, in this way, its constitutes a natural protective agent against alterations caused by current eating patterns.
publisher Universidad Nacional Córdoba. Facultad de Ciencias Médicas. Secretaria de Ciencia y Tecnología
publishDate 2023
url https://revistas.unc.edu.ar/index.php/med/article/view/42695
work_keys_str_mv AT leonm quercetinasnaturalstrategytoavoidplasmalipidimbalanceandintestinalmucosaoxidativestresscausedbyhighfructosediet
AT lobov quercetinasnaturalstrategytoavoidplasmalipidimbalanceandintestinalmucosaoxidativestresscausedbyhighfructosediet
AT lucianin quercetinasnaturalstrategytoavoidplasmalipidimbalanceandintestinalmucosaoxidativestresscausedbyhighfructosediet
AT salcedor quercetinasnaturalstrategytoavoidplasmalipidimbalanceandintestinalmucosaoxidativestresscausedbyhighfructosediet
AT moinel quercetinasnaturalstrategytoavoidplasmalipidimbalanceandintestinalmucosaoxidativestresscausedbyhighfructosediet
AT diazdebarbozag quercetinasnaturalstrategytoavoidplasmalipidimbalanceandintestinalmucosaoxidativestresscausedbyhighfructosediet
AT leonm quercetinacomoestrategianaturalparaevitareldesbalancelipidicoenplasmayelestresoxidativoenmucosaintestinalcausadosporunadietaaltaenfructosa
AT lobov quercetinacomoestrategianaturalparaevitareldesbalancelipidicoenplasmayelestresoxidativoenmucosaintestinalcausadosporunadietaaltaenfructosa
AT lucianin quercetinacomoestrategianaturalparaevitareldesbalancelipidicoenplasmayelestresoxidativoenmucosaintestinalcausadosporunadietaaltaenfructosa
AT salcedor quercetinacomoestrategianaturalparaevitareldesbalancelipidicoenplasmayelestresoxidativoenmucosaintestinalcausadosporunadietaaltaenfructosa
AT moinel quercetinacomoestrategianaturalparaevitareldesbalancelipidicoenplasmayelestresoxidativoenmucosaintestinalcausadosporunadietaaltaenfructosa
AT diazdebarbozag quercetinacomoestrategianaturalparaevitareldesbalancelipidicoenplasmayelestresoxidativoenmucosaintestinalcausadosporunadietaaltaenfructosa
first_indexed 2024-09-03T21:04:49Z
last_indexed 2024-09-03T21:04:49Z
_version_ 1809210397738139648
spelling I10-R327-article-426952023-10-19T21:19:51Z Quercetin as natural strategy to avoid plasma lipid imbalance and intestinal mucosa oxidative stress caused by high fructose diet Quercetina como estrategia natural para evitar el desbalance lipídico en plasma y el estrés oxidativo en mucosa intestinal causados por una dieta alta en fructosa León, M Lobo, V Luciani, N Salcedo, R Moine, L Díaz de Barboza, G High Fructose Diet quercetin oxidative stress dyslipidemia Dieta Alta en Fructosa quercetina estrés oxidativo dislipemia High Fructose Diet (HFD) is associated with development of metabolic syndrome, oxidative stress (OS) and inflammation. In recent decades, polyphenols have been postulated as strategic nutrients in antioxidant functional diets. Aim was to evaluate Quercetin (Q) ability to prevent OS in intestinal mucosa and to avoid glycemia and lipemia alterations generated by HFD. Wistar rats were divided into different study groups: Control (C, no treatment); subjected to HFD for 60 days (HFD) and rats treated with HFD and Q (25, 50 or 75 mg/kg/pc, HFD+Q) last 15 days of treatment. After animal’s sacrifice, glucose, HbA1c, triglycerides (TG), total cholesterol and HDL-cholesterol in blood and glutathione (GSH) content and superoxide dismutase (SOD) and catalase (CAT) activity in intestinal mucosa homogenate were determined. Results were analyzed by ANOVA/Tukey (p<0.05). All groups presented similar values of glycemia, HbA1c and total cholesterol. HFD rats had high TG levels and decreased HDL-cholesterol levels in relation to C (185±9.75 HFD* vs 90.75±7.26 C, *p<0.001 and 20±1.08 HFD* vs 26.25±1.65 C, *p<0.001), which was reflected in TG/HDL-col ratio increased, a cardiovascular risk and insulin resistance indicator (9.30±0, 57 HFD* vs 3.48±0.26 C, *p<0.001). Q evidences its protective effect by normalizing values of these parameters (TG/HDL-cholesterol: 7.29±0.42 HFD+Q25; 4.55±0.35 HFD+Q50; 5.60±0.30 HFD+Q75, *p<0.001 vs HFD). In relation to OS, we found that Q prevents SOD activity increment (31.22±3.5 HFD* vs 4.26±2.91 C; 9.85±4.03 HFD+Q25; 11.81±4.7 HFD+Q50, 17.59±3 HFD+Q75, *p<0.05) and CAT (45.85±5.26 HFD* vs 11.55±4.88 C, 17.34 ±3.98 HFD+Q25, 28.54±3.04 HFD+Q50, 17.67±3.89 HFD+Q75, *p<0.001). Added to this effect Q had ability to prevent GSH decrease in rat intestinal mucosa (3.93±0.14 HFD* vs 6.99±0.64 C, 7.22±0.55 HFD+ Q25 and 9.35±1.12 HFD+Q50, *p<0.05). Our results indicate that Q has a protective effect against dyslipidemia and OS in intestinal mucosa generated by high fructose consumption, in this way, its constitutes a natural protective agent against alterations caused by current eating patterns. Las Dietas Altas en Fructosa (DAF) están relacionadas al desarrollo de síndrome metabólico, estrés oxidativo (EO) e inflamación. En las últimas décadas, se ha postulado a los polifenoles como nutrientes estratégicos en una alimentación funcional antioxidante. El objetivo de este trabajo fue evaluar la capacidad de Quercetina (Q) para prevenir el EO en mucosa intestinal y evitar las alteraciones en glucemia y lipemia generadas por el consumo de DAF. Se emplearon ratas Wistar controles (C), sometidas a DAF por 60 días y ratas tratadas con DAF y Q (25, 50 ó 75 mg/kg/pc, DAF+Q) los últimos 15 días de tratamiento. Luego del sacrificio de los animales se determinó glucosa, HbA1c, triglicéridos (TG), colesterol total y HDL-col en sangre y contenido de glutatión (GSH) y actividad de superóxido dismutasa (SOD) y catalasa (CAT) en homogenizado de mucosa intestinal. Los resultados se analizaron mediante ANOVA/Tukey (p<0,05). Todos los grupos presentaron similares valores de glucemia, HbA1C y colesterol total. Las ratas que recibieron DAF presentaron elevados niveles de TG y disminución en los de HDL-col en relación a los C (185±9,75 DAF* vs 90,75±7,26 C, *p<0,001 y 20±1,08 DAF* vs 26,25±1,65 C, *p<0,001), lo que se refleja en un aumento de la relación TG/HDL-col, indicador de riesgo cardiovascular e insulino-resistencia (9,30±0,57 DAF* vs 3,48±0,26 C, *p<0,001). Q evidencia su efecto protector normalizando los valores de estos parámetros (TG/HDL-col: 7,29±0,42 DAF+Q25; 4,55±0,35 DAF+Q50; 5,60±0,30 DAF+Q75, *p<0,001 vs DAF). En relación al EO, se encontró que Q evita el incremento de la actividad de SOD (31,22±3,5 DAF* vs 4,26±2,91 C; 9,85±4,03 DAF+Q25; 11,81±4,7 DAF+Q50; 17,59±3 DAF+Q75, *p<0,05) y CAT (45,85±5,26 DAF* vs 11,55±4,88 C; 17,34±3,98 DAF+Q25; 28,54±3,04 DAF+Q50; 17,67±3,89 DAF+Q75, *p<0,001). A este efecto se suma además la capacidad de Q para evitar la disminución de GSH en mucosa intestinal de ratas (3,93±0,14 DAF* vs 6,99±0,64 C, 7,22±0,55 DAF+Q25 y 9,35±1,12 DAF+Q50, *p<0,05). Nuestros resultados indican que Q presenta un efecto protector frente a la dislipemia y al EO en mucosa intestinal generados por el consumo elevado de fructosa, constituyéndose en un agente natural protector frente a alteraciones ocasionadas por patrones alimentarios actuales.   Universidad Nacional Córdoba. Facultad de Ciencias Médicas. Secretaria de Ciencia y Tecnología 2023-10-19 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf https://revistas.unc.edu.ar/index.php/med/article/view/42695 Revista de la Facultad de Ciencias Médicas de Córdoba.; Vol. 80 (2023): Suplemento JIC XXIV Revista de la Facultad de Ciencias Médicas de Córdoba; Vol. 80 (2023): Suplemento JIC XXIV Revista da Faculdade de Ciências Médicas de Córdoba; v. 80 (2023): Suplemento JIC XXIV 1853-0605 0014-6722 spa https://revistas.unc.edu.ar/index.php/med/article/view/42695/42891 Derechos de autor 2023 Universidad Nacional de Córdoba http://creativecommons.org/licenses/by-nc/4.0

Ejemplares similares