A heteropaternal superfertilization case report in Cordoba City
Heteroparental superfertilization is an extremely rare phenomenon that occurs when two mature eggs are released at different times within the same menstrual cycle. They are fertilized by sperm from different parents in separate sexual intercourse. Although superfertilization is quite c...
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| Autores principales: | , , , , , , |
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| Formato: | Artículo revista |
| Lenguaje: | Español |
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Universidad Nacional Córdoba. Facultad de Ciencias Médicas. Secretaria de Ciencia y Tecnología
2023
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| Acceso en línea: | https://revistas.unc.edu.ar/index.php/med/article/view/42649 |
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| Sumario: | Heteroparental superfertilization is an extremely rare phenomenon that occurs when two mature eggs are released at different times within the same menstrual cycle. They are fertilized by sperm from different parents in separate sexual intercourse. Although superfertilization is quite common in the animal kingdom, it has rarely been described in humans. Due to its low frequency, accurate diagnostics in paternity disputes are key to avoid erroneous conclusions. The current availability of molecular methodologies and the increasing number of markers in paternity testing suggest that the number of cases of heteroparental superfertilization will increase over time and will no longer be an isolated event.
We present the case of four people who attended the service of the Pilot Center for the Detection of Metabolic Errors (CEPIDEM), requesting paternity tests on twins of different sexes. Samples were taken from the brothers, the undoubted mother and the alleged father. DNA extraction was performed using the CTAB lysis technique and purification with chloroform/isoamyl alcohol and precipitation with isopropanol. Subsequently, 13 STR (short tandem repeat) DNA markers were amplified using the PCR technique and the amplified products were analyzed using the Applied Biosystems ABI 310 sequencer. The statistical calculations indicated by the International Society of Forensic Genetics were performed, using a probability of 0.5 and the tables of allelic frequencies of the Cordoba population.
The genetic markers analyzed indicated exclusion as the biological father of one of the siblings studied, with a certainty of 100%. At the same time, the genetic markers indicated inclusion as the biological father of the other brother, with a certainty of 99.998%. The percentages of certainty demonstrate the effectiveness of molecular methods to solve complex filial relationships
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