Frecuencia etiológica de Aichivirus y caracterización molecular de cepas en casos de diarrea y en matrices ambientales de Córdoba, Argentina
Human Aichi viruses (AiV) belong to the Picornaviridae family, Kobuvirus genus. There are 3 genotypes: A, B and C. These viruses are transmitted by the fecal-oral route and are considered emerging agents of acute gastroenteritis (AGE). Objective: to study the presence of AiV in raw wastewater...
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| Autores principales: | , , , , , , , |
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| Formato: | Artículo revista |
| Lenguaje: | Español |
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Universidad Nacional Córdoba. Facultad de Ciencias Médicas. Secretaria de Ciencia y Tecnología
2023
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| Acceso en línea: | https://revistas.unc.edu.ar/index.php/med/article/view/42640 |
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| Sumario: | Human Aichi viruses (AiV) belong to the Picornaviridae family, Kobuvirus genus. There are 3 genotypes: A, B and C. These viruses are transmitted by the fecal-oral route and are considered emerging agents of acute gastroenteritis (AGE). Objective: to study the presence of AiV in raw wastewater (RA) samples as a reflection of viral circulation in the population and in children with AGE in the city of Córdoba, Argentina, and to characterize the viral genome in both types of samples.
RA samples (n= 59) from the treatment plant of the city of Córdoba (period: 2012-2013, 2015, 2018-2019) and fecal matter (MF) samples from children with AGE (n=155), period: 2012 (n=12), 2013 (n= 37), 2014 (n=18), 2015 (n=15), 2016 (n=1), 2017 (n=1), 2018 (n=18) and 2019 (n=53); assisted at the Reina Fabiola University Clinic of Cordoba (sentinel hospital), were analyzed. M and M. Viral concentration in RA: elution and precipitation with PEG-6000; AiV molecular detection in RA and MF: nested RT-PCR targeting the 3CD genomic region, sequencing, and phylogenetic analysis.
In RA, AiV was detected throughout the study period (61%) and without a seasonal pattern. In MF, the overall AiV detection frequency was 14.8% (23/155); 10.3% in mono-detection; 10.4% (11/107) in co-infection with rotavirus and 1% (1/107) in co-infection with adenovirus. No significant differences were observed between hospitalized and outpatient children. AiV was detected more frequently in males and in the group under five years old. The nucleotide identity between the AR and MF strains was 93.6% to 99.2%.
The high detection rates of AiV in RA and the detection of AiV in children with diarrhea would suggest that the virus circulates endemically in the population of Córdoba and that AiV infection is acquired early in life. Results obtained are the first data available for Argentina and challenge to carry out new studies on the presence of AiV in children with diarrhea, on the seroprevalence of AiV in the population and continue with the study of RA to obtain information on the circulation of this virus in the population.
Keywords:
Aichi virus, acute gastroenteritis, emergent virus
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