Direct immunofluorescence of SARS-CoV-2: implementation of a rapid diagnosis from Covid19 patient samples from Cordoba
The SARS-CoV-2 virus generates the COVID-19 disease, and early diagnosis is very important to prolong patient survival and ensure the safety of the population. The objective of this research was to implement a rapid diagnosis of SARS-CoV-2 based on a viral antigen detection technique by immunofluore...
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| Autores principales: | , , , , , , , , , , , , , , , , , , , , , |
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| Formato: | Artículo revista |
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Universidad Nacional Córdoba. Facultad de Ciencias Médicas. Secretaria de Ciencia y Tecnología
2022
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| Acceso en línea: | https://revistas.unc.edu.ar/index.php/med/article/view/39132 |
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| Sumario: | The SARS-CoV-2 virus generates the COVID-19 disease, and early diagnosis is very important to prolong patient survival and ensure the safety of the population. The objective of this research was to implement a rapid diagnosis of SARS-CoV-2 based on a viral antigen detection technique by immunofluorescence (DFI).
Covid-19(+) samples submitted by collaborating healthcare centers were processed following the recommended safety measures and a total of 165 positive and 59 negative imprints were performed. These imprints were applied to optimize/standardize the DFI following the usual procedure. Primary antibodies: polyclonal serum from convalescent patients; gamma globulins and monoclonal antibodies. Conjugates: goat polyclonal secondary antibodies against mouse and human IgG, and mouse against human IgG labeled with FITC. A total of 33 imprints were used for each primary antibody. Sensitivity, specificity, positive and negative predictive value (PPV) and (NPV) of the technique were determined using the statistical program R-medic.
The results show images with good fluorescence patterns defined for each primary antibody, together with the corresponding controls.The evaluation values of the technique were obtained by performing the analyses in comparison with the RT-PCR technique.The results were similar for post-infection serum and post-vaccinal serum: sensitivity of DFI was 82%, specificity was 64%, PPV was 82% and NPV was 64%; post-infection serum with antibody titer: sensitivity of DFI was 84%, specificity was 57%, PPV was 73% and NPV was 73%; Gamma globulin: DFI sensitivity was 85%, specificity was 67%, PPV was 92% and NPV was 50%; Monoclonal Anti-N and Anti-RBD: DFI sensitivity was 92% , specificity was 78%, PPV was 92% and NPV was 78%. Obtaining good values of sensitivity, specificity, PPV and NPV.
This research reports for the first time in Córdoba, the development of a direct diagnostic method of SARS-CoV-2 by DFI "in house", remarkably relevant to be applied in the initial screening as differential diagnosis when this infection is part of seasonal ARIs, going from pandemic to regional endemic. |
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