Arterial disfunction associated to experimental type 1 diabetes mellitus, protective effect of naringin
Diabetes mellitus (DM) is a risk factor for the development of systemic atherosclerosis and vascular calcification (VC). Chronic inflammation and oxidative stress might be the involved mechanisms in the VC. Hyperglycemia produces oxygen- and nitrogen-derived free radicals (ROS) and triggers endothel...
Guardado en:
| Autores principales: | , , , , , |
|---|---|
| Formato: | Artículo revista |
| Publicado: |
Universidad Nacional Córdoba. Facultad de Ciencias Médicas. Secretaria de Ciencia y Tecnología
2022
|
| Materias: | |
| Acceso en línea: | https://revistas.unc.edu.ar/index.php/med/article/view/39067 |
| Aporte de: |
| Sumario: | Diabetes mellitus (DM) is a risk factor for the development of systemic atherosclerosis and vascular calcification (VC). Chronic inflammation and oxidative stress might be the involved mechanisms in the VC. Hyperglycemia produces oxygen- and nitrogen-derived free radicals (ROS) and triggers endothelial cell (EC) dysfunction. Treatment with natural antioxidants is likely to prevent these complications. Aim: To evaluate the effect of Naringin (NAR) as a CV protector in an experimental model of type 1 DM.
Wistar rats were divided in controls (C), diabetic (STZ, treated with 60 mg of streptozotocin/kg b.w.) and diabetic treated with NAR (40 mg/kg b.w.). CICUAL:16/08/2018. The animals were sacrificed 30 days post-treatment after blood extraction and the aortas were obtained. Serum alkaline phosphatase (AP) activity and ROS level in red blood cells were determined. In histological sections of aorta, the morphology of the vascular wall was analyzed with H&E. In cultured aortic ECs, the NO• content, an indicator of vascular health, and the expression of the enzyme inducible nitric oxide synthase (iNOS) were quantified by immunohistochemistry. ANOVA and Tukey were used for statistical analysis (p<0.05).
AP activity and ROS level increased in STZ rats and returned to control values with NAR (AP: C:198.50±28.78; STZ:515.20±54.23*; NAR:227.80±46.43 IU/L; *p<0.001vs C and NAR. ROS: C:224.66±1.45; STZ:327.33±41.28*; NAR:218.33±17.37 AU; *p<0.05 vs C and NAR). NO• content and iNOS expression in ECs from diabetic rats decreased by 33 and 25%, respectively. NAR only normalized NO• values. Aortas from all groups were exposed to procalcifying medium for 7 days, then decalcified and the released calcium was quantified spectrophotometrically. Calcium content was higher in STZ rats and NAR normalized the values (C:8.7±0.5; STZ:15.1±0.8*; NAR:10.8±0.7 mg Ca2+/dL; *p<0.01 vs C and NAR). NAR partially prevented the decrease in aortas muscle fiber width in STZ group.
These results demonstrate that NAR improves vascular health, attenuates CV and restores NO• levels in a diabetes mellitus experimental model. |
|---|