Hight resolution optical microscopy of the cellular cycle in psoriasis patients.

Skin tissue from patients with Psoriasis was analyzed using HROM (High Resolution Optical Mlcroscopy), studying epithelial differentlation and posslble structural alterations of the queratinocytes. The samples were taken from 10 patients with histopathologlc diagnosis of Psoriasis. Thls tissue sampl...

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Autores principales: Frede, S, Cabalier, MED, Reinoso, N, Berrotarán, N, Juri, G, Piccinni, DJ, Asís, G, Burgos, E, Spitale, LS
Formato: Artículo revista
Lenguaje:Español
Publicado: Universidad Nacional Córdoba. Facultad de Ciencias Médicas. Secretaria de Ciencia y Tecnología 2019
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Acceso en línea:https://revistas.unc.edu.ar/index.php/med/article/view/26112
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Sumario:Skin tissue from patients with Psoriasis was analyzed using HROM (High Resolution Optical Mlcroscopy), studying epithelial differentlation and posslble structural alterations of the queratinocytes. The samples were taken from 10 patients with histopathologlc diagnosis of Psoriasis. Thls tissue samples where afflxed with glutaraldehide buffer- collidine for 48 hours. Later processed with the HROM technlque and colored with toluldine blue, metilene blue, basic Fuscine, and silver metenarnine. The basal epithelial elements presented ovoid nucleus and most of them had prominent nucleolus. In 7 of the studied cases, the granulose stratus was absent, and thinner in the rest, with nucleus and nucleolus retention. At thls level queratinocytes where observed with perinuclear anfofilia, as well as llnfocltic and macrophagic infiltrate and unlon complex where elongated.