The effect of oxidative stress on infection of trophoblast derived cells (BeWo) by Trypanosoma cruzi
Human placenta constitutes a physiological barrier against different pathogen microorganisms including Trypanosoma cruzi, which is the parasite that causes Chagas disease. During congenital transmission, the parasite breaks the placental barrier. The placental immune response has been shown...
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| Autores principales: | , , , , , , , |
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| Formato: | Artículo revista |
| Lenguaje: | Español |
| Publicado: |
Universidad Nacional Córdoba. Facultad de Ciencias Médicas. Secretaria de Ciencia y Tecnología
2019
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| Acceso en línea: | https://revistas.unc.edu.ar/index.php/med/article/view/25903 |
| Aporte de: |
| Sumario: | Human placenta constitutes a physiological barrier against different pathogen microorganisms including Trypanosoma cruzi, which is the parasite that causes Chagas disease. During congenital transmission, the parasite breaks the placental barrier. The placental immune response has been shown to exert a harmful effect on Trypanosoma cruzi (T. cruzi). Nitrosative/ oxidative stress prevents the invasion of microorganisms and fetal infection; it is currently unknown within the placental tissue which of the components thereof can perform this function. The aim of the present work was to evaluate the role of trophoblastic cells (BeWo cell line) and oxidative/ nitrosative stress in T. cruzi infection
In BeWo cells culture sincialization with Forskolina was induced and essays were performed in cytotrophoblast and syncytiotrophoblast cultured for 48 hours with H12 F12 medium at 37° C in a ratio 1:1 and 5:1 cells: parasites (n=4); they were treated with inhibitors of reactive oxygen species (NAC) and nitric oxide synthase NOS (L-NAME). The infection was analized by optical microscopy with GIEMSA staining. In culture supernatants, nitrites were measured through the Griess technique. Statically analysis was performed using ANAVA considering significant differences when p<0.05.
It was observed that the syncytiotrophoblast has significant lower infection to the cytotrophoblast with both concentrations of parasites; this decrease is reversed in cultures treated with NAC in both concentrations of parasites and with L-NAME with the lowest concentration of parasites. An increase in the concentration of nitrites was observed in the presence of both parasite concentrations in both cyto and syncytiotrophoblast.
The syncytiotrophoblast represents the first placental barrier and is less susceptible to infection than cytotrophoblast, with nitrosative/ oxidative stress being one of the placental protection factors against T. cruzi infection. We can conclude that both cyto and syncytiotrophoblast play a fundamental role in the immune response in the placental tissue, the syncytiotrophoblast being the first barrier and the one with lowest susceptibility to T. cruzi infection. |
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