Ghrelin effects on in vivo fertilization rate and litter size in mice
Male sexual behavior is highly regulated by neuroendocrine interactions. Testosterone is a key hormone for male sexual development and sexual behavior. Ghrelin (Ghrl) is a growth hormone secretagogue peptide that is being investigated for its implications on the reproductive system. In previous stid...
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| Autores principales: | , , , , , |
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| Formato: | Artículo revista |
| Lenguaje: | Español |
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Universidad Nacional Córdoba. Facultad de Ciencias Médicas. Secretaria de Ciencia y Tecnología
2019
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| Materias: | |
| Acceso en línea: | https://revistas.unc.edu.ar/index.php/med/article/view/25664 |
| Aporte de: |
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I10-R327-article-25664 |
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ojs |
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Universidad Nacional de Córdoba |
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I-10 |
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R-327 |
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Revista de la Facultad de Ciencias Médicas de Córdoba |
| language |
Español |
| format |
Artículo revista |
| topic |
reproduction ghrelin hypothalamus fertilitation rate embryonic loss reproducción ghrelina hipotálamo tasa de fertilizacion |
| spellingShingle |
reproduction ghrelin hypothalamus fertilitation rate embryonic loss reproducción ghrelina hipotálamo tasa de fertilizacion Poretti , MB Bianconi, S Martini, AC Luque , E Vincenti, L Carlini, VP Ghrelin effects on in vivo fertilization rate and litter size in mice |
| topic_facet |
reproduction ghrelin hypothalamus fertilitation rate embryonic loss reproducción ghrelina hipotálamo tasa de fertilizacion |
| author |
Poretti , MB Bianconi, S Martini, AC Luque , E Vincenti, L Carlini, VP |
| author_facet |
Poretti , MB Bianconi, S Martini, AC Luque , E Vincenti, L Carlini, VP |
| author_sort |
Poretti , MB |
| title |
Ghrelin effects on in vivo fertilization rate and litter size in mice |
| title_short |
Ghrelin effects on in vivo fertilization rate and litter size in mice |
| title_full |
Ghrelin effects on in vivo fertilization rate and litter size in mice |
| title_fullStr |
Ghrelin effects on in vivo fertilization rate and litter size in mice |
| title_full_unstemmed |
Ghrelin effects on in vivo fertilization rate and litter size in mice |
| title_sort |
ghrelin effects on in vivo fertilization rate and litter size in mice |
| description |
Male sexual behavior is highly regulated by neuroendocrine interactions. Testosterone is a key hormone for male sexual development and sexual behavior. Ghrelin (Ghrl) is a growth hormone secretagogue peptide that is being investigated for its implications on the reproductive system. In previous stidies, our group has demonstrated that Ghrl chronic administration decreases plasma testosterone concentration and affects quality and concentration of sperm. That is why we studied the impact of intrahypothalamic Ghrl treatment on fertilization index in vivo and litter size.
Males were treated for 42 days with cerebrospinal fluid (control) or Ghrl 3.0 nmol/day administered in the hypothalamus and 3 days before the end of treatment, males were housed with untreated females on the afternoon of proestrus and the existence of copulation was analyzed. 18 days after copulation, offspring number and litter size was analyzed. Additionally, reproductive function of treated animals was evaluated in vitro, using untreated females. The data were analyzed by ANOVA followed by an LSD test, N = 6-8 animals / group.
Treatment with Ghrl 3.0 nmol / day significantly increases the percentage of embryonic loss (F = 2.58, df = 2, p <0.05) and the number of atrophied fetuses (F = 2.24, df = 2, p <0.05) in females that copulated with treated animals. Therewere no significant differences between other parameters evaluated (in vivo fertilization rate, number of fetuses / litter size, litter weight, average weight of the offspring, number of corpus lutea). No significant differences were observed in the percentage of oocytes fertilized in vitro in animals treated with Ghrl with respect to control animals (p> 0.05).
Ghrelin treatment did not affect the fertilization rate, however, it produced an increase in the percentage of embryonic loss in vivo |
| publisher |
Universidad Nacional Córdoba. Facultad de Ciencias Médicas. Secretaria de Ciencia y Tecnología |
| publishDate |
2019 |
| url |
https://revistas.unc.edu.ar/index.php/med/article/view/25664 |
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I10-R327-article-256642024-08-27T18:26:03Z Ghrelin effects on in vivo fertilization rate and litter size in mice Efectos de la administración de Ghrelina sobre el índice de fertilización in vivo y tamaño de la camada en ratones Poretti , MB Bianconi, S Martini, AC Luque , E Vincenti, L Carlini, VP reproduction ghrelin hypothalamus fertilitation rate embryonic loss reproducción ghrelina hipotálamo tasa de fertilizacion Male sexual behavior is highly regulated by neuroendocrine interactions. Testosterone is a key hormone for male sexual development and sexual behavior. Ghrelin (Ghrl) is a growth hormone secretagogue peptide that is being investigated for its implications on the reproductive system. In previous stidies, our group has demonstrated that Ghrl chronic administration decreases plasma testosterone concentration and affects quality and concentration of sperm. That is why we studied the impact of intrahypothalamic Ghrl treatment on fertilization index in vivo and litter size. Males were treated for 42 days with cerebrospinal fluid (control) or Ghrl 3.0 nmol/day administered in the hypothalamus and 3 days before the end of treatment, males were housed with untreated females on the afternoon of proestrus and the existence of copulation was analyzed. 18 days after copulation, offspring number and litter size was analyzed. Additionally, reproductive function of treated animals was evaluated in vitro, using untreated females. The data were analyzed by ANOVA followed by an LSD test, N = 6-8 animals / group. Treatment with Ghrl 3.0 nmol / day significantly increases the percentage of embryonic loss (F = 2.58, df = 2, p <0.05) and the number of atrophied fetuses (F = 2.24, df = 2, p <0.05) in females that copulated with treated animals. Therewere no significant differences between other parameters evaluated (in vivo fertilization rate, number of fetuses / litter size, litter weight, average weight of the offspring, number of corpus lutea). No significant differences were observed in the percentage of oocytes fertilized in vitro in animals treated with Ghrl with respect to control animals (p> 0.05). Ghrelin treatment did not affect the fertilization rate, however, it produced an increase in the percentage of embryonic loss in vivo El comportamiento sexual masculino se encuentra altamente regulado por interacciones neuroendocrinas. La testosterona es una hormona clave para el desarrollo sexual masculino y la conducta sexual. Ghrelina (Ghrl) es un péptido secretagogo de la hormona de crecimiento que está siendo estudiado por sus implicancias sobre el sistema reproductor. En estudios previos, nuestro grupo ha demostrado que la administración crónica de Ghrl disminuye la concentración de testosterona en plasma y afecta la calidad y concentración de espermatozoides. Es por ello que se evalúa el impacto de la administración intrahipotalámica(Ht) de Ghrl sobre el índice de fertilización in vivo y el tamaño de la camada. Para ello, se emplearon machos tratados hipotalámicamente durante 42 días con liquido cefaloraquídeo (control) o Ghrl3,0 nmol/día y 3 días antes de que finalice el tratamiento, los machos fueron alojados con hembras no tratadas la tarde del proestro y se analizó la existencia de cópula y 18 días después el número de crías y tamaño de la camada. Adicionalmente se evaluó la función reproductiva in vitro de los machos tratados, empleando hembras no tratadas. Los datos fueron analizados por ANOVA seguido por un test de LSD, N = 6-8 animales/grupo. El tratamiento con Ghrl 3,0 nmol/día en los machos, incrementa significativamente el número de fetos atrofiados en las hembras que copularon con estos animales (F = 2,24; df = 2; p <0,05). Este incremento produce un aumento significativo en el porcentaje de pérdida embrionaria (F = 2,58; df = 2; p <0,05). No hubo diferencias significativas entre los grupos en los otros parámetros evaluados (tasa de fertilización in vivo, número de fetos/tamaño de la camada, peso de la camada, peso promedio de las crías, número de cuerpos lúteos). No se observaron diferencias significativas en el porcentaje de ovocitos fertilizados in vitro en los animales tratados con Ghrl con respecto a los animales control (p> 0,05). El tratamiento con Ghrl no afectó la tasa de fertilización, sin embargo, produjo un incremento en el porcentaje de pérdida embrionaria in vivo. Universidad Nacional Córdoba. Facultad de Ciencias Médicas. Secretaria de Ciencia y Tecnología 2019-10-10 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf https://revistas.unc.edu.ar/index.php/med/article/view/25664 Revista de la Facultad de Ciencias Médicas de Córdoba.; 2019: Suplemento JIC XX Revista de la Facultad de Ciencias Médicas de Córdoba; 2019: Suplemento JIC XX Revista da Faculdade de Ciências Médicas de Córdoba; 2019: Suplemento JIC XX 1853-0605 0014-6722 10.31053/1853.0605.v76.nSuplemento spa https://revistas.unc.edu.ar/index.php/med/article/view/25664/27378 Derechos de autor 2019 Universidad Nacional de Córdoba https://creativecommons.org/licenses/by-nc/4.0 |