Diabetic nephropathy in eSS rats and their modulation with polyunsaturated fatty acids (ω6 / ω3) and the antioxidant nordehydroguaiaretic acid
Diabetes is a systemic and chronic disease; among its most important complications is nephropathy. Chronic inflammatory status and increased oxidative stress produce glomerular lesions in relation to the severity of the disease. Proteinuria is increased considering it a marker of kidney dam...
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| Formato: | Artículo revista |
| Lenguaje: | Español |
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Universidad Nacional Cba. Facultad de Ciencias Médicas. Secretaria de Ciencia y Tecnología
2019
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| Materias: | |
| Acceso en línea: | https://revistas.unc.edu.ar/index.php/med/article/view/25833 |
| Aporte de: |
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I10-R10article-25833 |
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ojs |
| institution |
Universidad Nacional de Córdoba |
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I-10 |
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R-10 |
| container_title_str |
Revistas de la UNC |
| language |
Español |
| format |
Artículo revista |
| topic |
type 2 diabetes mellitus diabetic nephropathy eSS rats polyunsaturated fatty acids nordehydroguaiareic acid diabetes mellitus Tipo 2 nefropatía diabética ratas eSS acidos grasos poliinsaturados ácido nordehidroguaiarético |
| spellingShingle |
type 2 diabetes mellitus diabetic nephropathy eSS rats polyunsaturated fatty acids nordehydroguaiareic acid diabetes mellitus Tipo 2 nefropatía diabética ratas eSS acidos grasos poliinsaturados ácido nordehidroguaiarético Bertozzi, J Repossi, G Díaz Gerevini, GT García, N López, C Diabetic nephropathy in eSS rats and their modulation with polyunsaturated fatty acids (ω6 / ω3) and the antioxidant nordehydroguaiaretic acid |
| topic_facet |
type 2 diabetes mellitus diabetic nephropathy eSS rats polyunsaturated fatty acids nordehydroguaiareic acid diabetes mellitus Tipo 2 nefropatía diabética ratas eSS acidos grasos poliinsaturados ácido nordehidroguaiarético |
| author |
Bertozzi, J Repossi, G Díaz Gerevini, GT García, N López, C |
| author_facet |
Bertozzi, J Repossi, G Díaz Gerevini, GT García, N López, C |
| author_sort |
Bertozzi, J |
| title |
Diabetic nephropathy in eSS rats and their modulation with polyunsaturated fatty acids (ω6 / ω3) and the antioxidant nordehydroguaiaretic acid |
| title_short |
Diabetic nephropathy in eSS rats and their modulation with polyunsaturated fatty acids (ω6 / ω3) and the antioxidant nordehydroguaiaretic acid |
| title_full |
Diabetic nephropathy in eSS rats and their modulation with polyunsaturated fatty acids (ω6 / ω3) and the antioxidant nordehydroguaiaretic acid |
| title_fullStr |
Diabetic nephropathy in eSS rats and their modulation with polyunsaturated fatty acids (ω6 / ω3) and the antioxidant nordehydroguaiaretic acid |
| title_full_unstemmed |
Diabetic nephropathy in eSS rats and their modulation with polyunsaturated fatty acids (ω6 / ω3) and the antioxidant nordehydroguaiaretic acid |
| title_sort |
diabetic nephropathy in ess rats and their modulation with polyunsaturated fatty acids (ω6 / ω3) and the antioxidant nordehydroguaiaretic acid |
| description |
Diabetes is a systemic and chronic disease; among its most important complications is nephropathy. Chronic inflammatory status and increased oxidative stress produce glomerular lesions in relation to the severity of the disease. Proteinuria is increased considering it a marker of kidney damage. There is evidence that polyunsaturated fatty acids (PUFAs) and antioxidants could modulate, at least in part, these processes. The eSS rats spontaneously develop type 2 diabetes, being a valuable experimental model. The aim of the present work was to analyze levels of oxidative stress markers, inflammatory and morphological alterations in kidneys of eSS rats treated with PUFAs and nordehydroguaiaretic acid (NDGA), comparing them with those of eSS rats without treatment and healthy controls.
Male rats were used: 4 Wistar (healthy controls) and 20 eSS that were divided into 5 groups (n=4 each), control groups received only the saline solution used as vehicle and the others received intraperitoneally, monthly for 12 months, 6.25mg / kg of PUFAs ω6 or ω3 with / without the addition of 1.9mg / kg of NDGA. Plasmatic metabolic markers (glycaemia, HbA1c), inflammation (reactive prot C, IL-6) and oxidative stress (GGTP) in renal tissue were determined using spectrophotometric techniques. Histopathological study of the kidneys was performed. The data obtained were analyzed by ANOVA and Tukey test, (p≤0.05).
In the eSS rats significant increases were found in fasting blood glucose (eSS rats ≥129 vs 107.8 mg / dl in Wistar), HbA1c (≥7.7% eSS vs 5% Wistar) and Reactive C Protein (Control groups eSS and ω6 ≥7.6 mg / dl; ω6 + NDGA and ω3 ≈4.6 mg / dl; ω3 + NDGA 3.4 mg / dl and Wistar 0.3 mg / dl). IL-6 levels were higher (2.5 to 3.7 times) in kidney than in plasma. The ω3+NDGA treatment reduced the values of IL-6, 12 to 72% higher, observed in eSS rats. GGTP activity was higher (52%) in eSS Control than Wistar, the treatments used were able to reduce it. Histological abnormalities were observed in the kidneys of eSS rats. |
| publisher |
Universidad Nacional Cba. Facultad de Ciencias Médicas. Secretaria de Ciencia y Tecnología |
| publishDate |
2019 |
| url |
https://revistas.unc.edu.ar/index.php/med/article/view/25833 |
| work_keys_str_mv |
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Revistas |
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