Impact of metabolic syndrome on murine fetal programming

In Argentina, metabolic syndrome (MS) is a highly prevalent pathology (30%), even during pregnancy. There exist several studies that investigate maternal malnutrition (hypo/ hypernutrition) on offspring development, showing that perinatal nutritional challenges exert long-lasting consequences...

Descripción completa

Detalles Bibliográficos
Autores principales: Gerbaldo, V, Ramirez , N, Torres, P, Jones, XM, Arja , A, Martini, AC, Luque, EM
Formato: Artículo revista
Lenguaje:Español
Publicado: Universidad Nacional Cba. Facultad de Ciencias Médicas. Secretaria de Ciencia y Tecnología 2019
Materias:
Acceso en línea:https://revistas.unc.edu.ar/index.php/med/article/view/25619
Aporte de:R de Universidad Nacional de Córdoba Ver origen
id I10-R10article-25619
record_format ojs
institution Universidad Nacional de Córdoba
institution_str I-10
repository_str R-10
container_title_str Revistas de la UNC
language Español
format Artículo revista
topic syndrome; fetal programming; postnatal development; reproduction
síndrome metabólico; programación fetal; desarrollo posnatal; reproducción
spellingShingle syndrome; fetal programming; postnatal development; reproduction
síndrome metabólico; programación fetal; desarrollo posnatal; reproducción
Gerbaldo, V
Ramirez , N
Torres, P
Jones, XM
Arja , A
Martini, AC
Luque, EM
Impact of metabolic syndrome on murine fetal programming
topic_facet syndrome; fetal programming; postnatal development; reproduction
síndrome metabólico; programación fetal; desarrollo posnatal; reproducción
author Gerbaldo, V
Ramirez , N
Torres, P
Jones, XM
Arja , A
Martini, AC
Luque, EM
author_facet Gerbaldo, V
Ramirez , N
Torres, P
Jones, XM
Arja , A
Martini, AC
Luque, EM
author_sort Gerbaldo, V
title Impact of metabolic syndrome on murine fetal programming
title_short Impact of metabolic syndrome on murine fetal programming
title_full Impact of metabolic syndrome on murine fetal programming
title_fullStr Impact of metabolic syndrome on murine fetal programming
title_full_unstemmed Impact of metabolic syndrome on murine fetal programming
title_sort impact of metabolic syndrome on murine fetal programming
description In Argentina, metabolic syndrome (MS) is a highly prevalent pathology (30%), even during pregnancy. There exist several studies that investigate maternal malnutrition (hypo/ hypernutrition) on offspring development, showing that perinatal nutritional challenges exert long-lasting consequences in the development and reproductive function of the offspring. It remains unknown, if MS exerts similar effects. Using a rat model of induced MS, we aim to investigate whether this pathology is a reproductive fetal programmer. Female Wistar rats (60 days old) were randomly divided into two groups: a) controls (C): pelleted chow + water or, b) MS: pelleted chow + 10% fructose (v/v) in water, which is a validated MS inductor protocol. Treatment was applied from 4 weeks before intercourse to weaning of the offspring (postnatal day 21). MS was verified in an initial group of dams (5 dams/treatment) with statistically higher values ​​(p <0.05) of total cholesterol (mg/dl) (MS= 87.9±1.6 vs C= 65.0±6.4), triglycerides (mg dl) (MS= 136.2±18.3 vs C= 79.5±11.9) and LDL (mg/dl) (MS= 24.3±4.5 vs C= 9.8±3.5). Furthermore, SM dams gain significantly more weight (g) (MS= 35.5±3.4 vs C= 23.9±3.7) and exhibited more visceral fat (g) (MS= 11.7±2.3 vs C= 6.4±1.3). MS did neither modify pregnancy duration, litter size, nor pups weigth at birth. In the offspring, MS tended to accelerate the onset of somatic and neurobiological parameters, and to increase more body weight gain than C. Furthermore, MS significantly advanced vaginal opening (on day 28: MS= 46.7±29.0% vs C= 10.0±5.0%) and testicular descent (on day 19: MS= 100.0±0.0% vs C= 11.67±5.43%; n=3- 4 litters/treatment; p <0.05). At adulthood, no differences were observed in male reproductive function. In females from MS dams, an increase in ovulation index was observed (MS= 15.0±0.4; C=12.23±0.5; n=10-12 females/group, p<0.05). Besides, 100% of the SM female litter lost at least one embryo vs 18% of C females. We are currently exploring the cellular/molecular bases of these results.  
publisher Universidad Nacional Cba. Facultad de Ciencias Médicas. Secretaria de Ciencia y Tecnología
publishDate 2019
url https://revistas.unc.edu.ar/index.php/med/article/view/25619
work_keys_str_mv AT gerbaldov impactofmetabolicsyndromeonmurinefetalprogramming
AT ramirezn impactofmetabolicsyndromeonmurinefetalprogramming
AT torresp impactofmetabolicsyndromeonmurinefetalprogramming
AT jonesxm impactofmetabolicsyndromeonmurinefetalprogramming
AT arjaa impactofmetabolicsyndromeonmurinefetalprogramming
AT martiniac impactofmetabolicsyndromeonmurinefetalprogramming
AT luqueem impactofmetabolicsyndromeonmurinefetalprogramming
AT gerbaldov impactodelsindromemetabolicoenlaprogramacionfetalmurina
AT ramirezn impactodelsindromemetabolicoenlaprogramacionfetalmurina
AT torresp impactodelsindromemetabolicoenlaprogramacionfetalmurina
AT jonesxm impactodelsindromemetabolicoenlaprogramacionfetalmurina
AT arjaa impactodelsindromemetabolicoenlaprogramacionfetalmurina
AT martiniac impactodelsindromemetabolicoenlaprogramacionfetalmurina
AT luqueem impactodelsindromemetabolicoenlaprogramacionfetalmurina
_version_ 1734617767272775680
spelling I10-R10article-256192019-11-11T21:18:27Z Impact of metabolic syndrome on murine fetal programming Impacto del síndrome metabólico en la programación fetal murina Gerbaldo, V Ramirez , N Torres, P Jones, XM Arja , A Martini, AC Luque, EM syndrome; fetal programming; postnatal development; reproduction síndrome metabólico; programación fetal; desarrollo posnatal; reproducción In Argentina, metabolic syndrome (MS) is a highly prevalent pathology (30%), even during pregnancy. There exist several studies that investigate maternal malnutrition (hypo/ hypernutrition) on offspring development, showing that perinatal nutritional challenges exert long-lasting consequences in the development and reproductive function of the offspring. It remains unknown, if MS exerts similar effects. Using a rat model of induced MS, we aim to investigate whether this pathology is a reproductive fetal programmer. Female Wistar rats (60 days old) were randomly divided into two groups: a) controls (C): pelleted chow + water or, b) MS: pelleted chow + 10% fructose (v/v) in water, which is a validated MS inductor protocol. Treatment was applied from 4 weeks before intercourse to weaning of the offspring (postnatal day 21). MS was verified in an initial group of dams (5 dams/treatment) with statistically higher values ​​(p <0.05) of total cholesterol (mg/dl) (MS= 87.9±1.6 vs C= 65.0±6.4), triglycerides (mg dl) (MS= 136.2±18.3 vs C= 79.5±11.9) and LDL (mg/dl) (MS= 24.3±4.5 vs C= 9.8±3.5). Furthermore, SM dams gain significantly more weight (g) (MS= 35.5±3.4 vs C= 23.9±3.7) and exhibited more visceral fat (g) (MS= 11.7±2.3 vs C= 6.4±1.3). MS did neither modify pregnancy duration, litter size, nor pups weigth at birth. In the offspring, MS tended to accelerate the onset of somatic and neurobiological parameters, and to increase more body weight gain than C. Furthermore, MS significantly advanced vaginal opening (on day 28: MS= 46.7±29.0% vs C= 10.0±5.0%) and testicular descent (on day 19: MS= 100.0±0.0% vs C= 11.67±5.43%; n=3- 4 litters/treatment; p <0.05). At adulthood, no differences were observed in male reproductive function. In females from MS dams, an increase in ovulation index was observed (MS= 15.0±0.4; C=12.23±0.5; n=10-12 females/group, p<0.05). Besides, 100% of the SM female litter lost at least one embryo vs 18% of C females. We are currently exploring the cellular/molecular bases of these results.   En nuestro país, el síndrome metabólico (SM) constituye una patología de alta prevalencia (30%), con alta incidencia durante la gestación. Modelos animales de hiper o hiponutrición materna han demostrado que los desafíos nutricionales perinatales tienen consecuencias a largo plazo en el desarrollo posnatal de las crías y su reproducción en la adultez, pero no se conoce si el SM ejerce efectos similares. Empleando un modelo de SM inducido en rata, nos propusimos investigar si esta patología constituye un programador fetal reproductivo. Utilizamos ratas Wistar hembras adultas (de 60 días), alimentadas con una dieta comercial, que se dividieron en dos grupos: a) controles (C) (comida balanceada + agua) y b) SM: comida balanceada + agua con 10% de fructosa (v/v; tratamiento inductor de SM). El tratamiento se mantuvo desde 4 semanas previas a la cópula y hasta el destete de las crías (día 21 posnatal). El SM se verificó en las madres con valores estadísticamente mayores (p<0,05) de colesterol total (mg/dl) (SM=87,9±1,6 vs C=65,0±6,4), triglicéridos (mg/dl)  (SM=136,2±18,3 vs C=79,5±11,9) y LDL (mg/dl) (SM=24,3±4,5 vs C=9,8±3,5). Además ganaron significativamente más peso (g) (SM=35,5±3,4 vs C=23,9±3,7) y presentaron  más grasa visceral (g) (SM=11,7±2,3 vs C=6,4±1,3). El SM no modificó significativamente la duración de la gestación, el número de crías, ni el peso inicial de la camada. En las crías, tendió a adelantar la aparición de los parámetros somáticos y neurobiológicos, concomitantemente con una tendencia a ganar mayor peso corporal que las crías C. El SM adelantó significativamente la apertura vaginal (en día 28: SM=46,7±29,0% vs C=10,0±5,0%) y el descenso testicular (en el día 19: SM=100,0±0,0% vs C=11,67±5,43%; n=3-4 camadas/tratamiento; p<0,05). En cuanto a la función reproductiva de las crías adultas, los machos del grupo SM no presentaron alteraciones de la calidad espermática. Las hembras, exhibieron mayor número de cuerpos lúteos (SM=15,00±0,4; C=12,25±0,5; n=10-12/grupo, p<0,05) y el 100% de las hembras gestadas bajo SM presentaron al menos una pérdida embrionaria vs las C, que sólo exhibieron 18%. Actualmente nos encontramos ahondando en las bases celulares/moleculares de estos resultados.   Universidad Nacional Cba. Facultad de Ciencias Médicas. Secretaria de Ciencia y Tecnología 2019-10-08 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf https://revistas.unc.edu.ar/index.php/med/article/view/25619 Revista de la Facultad de Ciencias Médicas de Córdoba; 2019: Suplemento JIC XX Revista da Faculdade de Ciências Médicas de Córdoba; 2019: Suplemento JIC XX 1853-0605 0014-6722 spa https://revistas.unc.edu.ar/index.php/med/article/view/25619/27386 Derechos de autor 2019 Universidad Nacional de Córdoba