Naringin attenuates the vascular calcification in a model of experimental Diabetes mellitus

Vascular calcification (VC) is one important complication of type 1 Diabetes mellitus (DM). Several studies suggest that the antioxidant naringin (NAR) supplementation is beneficial for the treatment of DM, but its effect on the VC has not been investigated. The aim of this work was to know whe...

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Autores principales: Rivoira, MA, Rauschemberger, MB, Rodriguez, VA
Formato: Artículo revista
Lenguaje:Español
Publicado: Universidad Nacional Cba. Facultad de Ciencias Médicas. Secretaria de Ciencia y Tecnología 2019
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Acceso en línea:https://revistas.unc.edu.ar/index.php/med/article/view/25571
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id I10-R10article-25571
record_format ojs
institution Universidad Nacional de Córdoba
institution_str I-10
repository_str R-10
container_title_str Revistas de la UNC
language Español
format Artículo revista
topic naringin; vascular calcification; experimental diabetes mellitus
naringina; calcificación vascular; diabetes mellitus experimental
spellingShingle naringin; vascular calcification; experimental diabetes mellitus
naringina; calcificación vascular; diabetes mellitus experimental
Rivoira, MA
Rauschemberger, MB
Rodriguez, VA
Naringin attenuates the vascular calcification in a model of experimental Diabetes mellitus
topic_facet naringin; vascular calcification; experimental diabetes mellitus
naringina; calcificación vascular; diabetes mellitus experimental
author Rivoira, MA
Rauschemberger, MB
Rodriguez, VA
author_facet Rivoira, MA
Rauschemberger, MB
Rodriguez, VA
author_sort Rivoira, MA
title Naringin attenuates the vascular calcification in a model of experimental Diabetes mellitus
title_short Naringin attenuates the vascular calcification in a model of experimental Diabetes mellitus
title_full Naringin attenuates the vascular calcification in a model of experimental Diabetes mellitus
title_fullStr Naringin attenuates the vascular calcification in a model of experimental Diabetes mellitus
title_full_unstemmed Naringin attenuates the vascular calcification in a model of experimental Diabetes mellitus
title_sort naringin attenuates the vascular calcification in a model of experimental diabetes mellitus
description Vascular calcification (VC) is one important complication of type 1 Diabetes mellitus (DM). Several studies suggest that the antioxidant naringin (NAR) supplementation is beneficial for the treatment of DM, but its effect on the VC has not been investigated. The aim of this work was to know whether NAR could attenuate the VC in Wistar male rats with DM. Three groups of animals were used: 1) controls, 2) diabetic rats (treated with 60 mg streptozotocin /kg b.w.: STZ), 3) diabetic rats treated with NAR (40 mg/kg b.w.). After 30 days of treatment, plasma was withdrawn and rats were sacrificed to obtain the aortas. Endothelial cells (EC) from aortas were cultured and NO•, indicator of vascular health, was measured by the Griess´s method. NO• production was significantly reduced in STZ rats, which was highly blocked by NAR (213,40 ± 33.3; 143,69±19.88*; 184,66±11.99; C; STZ; STZ + NAR 40; *p<0.01). In control aortas, estrona (E1) and genistein (Gen) stimulate NO• synthesis via estrogen receptor, but in aortas from STZ rats there is lack of NO• stimulation by those hormones. However, NAR restores the capability to stimulate NO• production under E1 and Gen. Isolated aortas from the different groups of animals were exposed to a pro-calcific medium with glicerophosphate for 7 days; the aortas were decalcified and the released calcium was measured by a commercial kit. Calcium content from aortas of STZ rats was 74% higher (p< 0.01) than that from the control rats. NAR treatment reduced calcium incorporation to values closed to the control ones.  These data were confirmed by AgNO3 staining. Aortas from STZ rats showed multiple sites of calcification, effect that was abolished by NAR treatment. All data suggest that NAR could prevent damage of the vascular architecture and functionality in diabetic rats.
publisher Universidad Nacional Cba. Facultad de Ciencias Médicas. Secretaria de Ciencia y Tecnología
publishDate 2019
url https://revistas.unc.edu.ar/index.php/med/article/view/25571
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AT rauschembergermb naringinattenuatesthevascularcalcificationinamodelofexperimentaldiabetesmellitus
AT rodriguezva naringinattenuatesthevascularcalcificationinamodelofexperimentaldiabetesmellitus
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AT rauschembergermb naringinaatenualacalcificacionvascularenunmodelodediabetesmellitusexperimental
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