Effects of conditioned media from fibroblasts stimulated by dietary components on breast tumoral cells
Published evidence suggests a strong association between nutrition and the development and maintenance of tumoral processes. In Córdoba, a greater adherence to dietary patterns (DP) provided by carbohydrates- and saturated fatty acids-rich food, represented mainly by fructose (F) and palmitic acid (...
Guardado en:
| Autores principales: | , , , , |
|---|---|
| Formato: | Artículo revista |
| Publicado: |
Universidad Nacional Cba. Facultad de Ciencias Médicas. Secretaria de Ciencia y Tecnología
2022
|
| Materias: | |
| Acceso en línea: | https://revistas.unc.edu.ar/index.php/med/article/view/34850 |
| Aporte de: |
| id |
I10-R10-article-34850 |
|---|---|
| record_format |
ojs |
| institution |
Universidad Nacional de Córdoba |
| institution_str |
I-10 |
| repository_str |
R-10 |
| container_title_str |
Revistas de la UNC |
| format |
Artículo revista |
| topic |
Carbohydrates fatty acids tumor microenvironment fibroblasts Carbohidratos ácidos grasos microambiente tumoral fibroblastos . |
| spellingShingle |
Carbohydrates fatty acids tumor microenvironment fibroblasts Carbohidratos ácidos grasos microambiente tumoral fibroblastos . Solla , ED Sosa, LDV Ferrero , V Pasqualini , ME Quintar , AA Effects of conditioned media from fibroblasts stimulated by dietary components on breast tumoral cells |
| topic_facet |
Carbohydrates fatty acids tumor microenvironment fibroblasts Carbohidratos ácidos grasos microambiente tumoral fibroblastos . |
| author |
Solla , ED Sosa, LDV Ferrero , V Pasqualini , ME Quintar , AA |
| author_facet |
Solla , ED Sosa, LDV Ferrero , V Pasqualini , ME Quintar , AA |
| author_sort |
Solla , ED |
| title |
Effects of conditioned media from fibroblasts stimulated by dietary components on breast tumoral cells |
| title_short |
Effects of conditioned media from fibroblasts stimulated by dietary components on breast tumoral cells |
| title_full |
Effects of conditioned media from fibroblasts stimulated by dietary components on breast tumoral cells |
| title_fullStr |
Effects of conditioned media from fibroblasts stimulated by dietary components on breast tumoral cells |
| title_full_unstemmed |
Effects of conditioned media from fibroblasts stimulated by dietary components on breast tumoral cells |
| title_sort |
effects of conditioned media from fibroblasts stimulated by dietary components on breast tumoral cells |
| description |
Published evidence suggests a strong association between nutrition and the development and maintenance of tumoral processes. In Córdoba, a greater adherence to dietary patterns (DP) provided by carbohydrates- and saturated fatty acids-rich food, represented mainly by fructose (F) and palmitic acid (PA), is positively associated with the risk of colorectal and breast cancer. However, the cellular mechanisms by which these components promote tumor aggressiveness are not fully understood. Our objective was to evaluate in vitro the role of tumor-associated fibroblasts (CAFs) as mediators of the proliferative effects of F and PA. F88 cell line, corresponding to human breast cancer CAFs, was grown in DMEM with 10% FBS and stimulated with F 40 mM, AP 250 uM, combinations of both (F+PA) or their respective vehicles, for 24 hours (characteristic concentrations of DP in Cordoba). Subsequently, supernatants were removed, total proteins were quantified and stored at -80 °C for assays. Breast epithelial tumor cells of the MCF7 line were cultured in DMEM medium with 10% FBS and then stimulated with conditioned media (from F88 cells) for 24 hours. As controls, conditioned media from vehicle-treated cells were used.
Any of the stimuli did not cause statistically significant changes in F88 cell proliferation. F + AP combination did induce a strong phenotypic change, with greater development of proteinopoietic and secretory organelles when observed by transmission electron microscopy. In addition, stimuli with PA and F + PA produced a decrease in the expression of Fibroblast Activation Protein (FAP) by western blot. On the other hand, MCF7 cells when stimulated with conditioned media from F88 treated with F + PA showed an increase in cell proliferation (ANOVA, p <0.05), determined by incorporation of bromodeoxyuridine and cell count. However, there were no significant differences between F and PA individually.
These results suggest a pathogenic effect of food rich in F and PA on tumor proliferation in breast cancer, which would be mediated by CAFs. |
| publisher |
Universidad Nacional Cba. Facultad de Ciencias Médicas. Secretaria de Ciencia y Tecnología |
| publishDate |
2022 |
| url |
https://revistas.unc.edu.ar/index.php/med/article/view/34850 |
| work_keys_str_mv |
AT sollaed effectsofconditionedmediafromfibroblastsstimulatedbydietarycomponentsonbreasttumoralcells AT sosaldv effectsofconditionedmediafromfibroblastsstimulatedbydietarycomponentsonbreasttumoralcells AT ferrerov effectsofconditionedmediafromfibroblastsstimulatedbydietarycomponentsonbreasttumoralcells AT pasqualinime effectsofconditionedmediafromfibroblastsstimulatedbydietarycomponentsonbreasttumoralcells AT quintaraa effectsofconditionedmediafromfibroblastsstimulatedbydietarycomponentsonbreasttumoralcells AT sollaed efectosdemedioscondicionadosdefibroblastosestimuladosporcomponentesdietariossobrecelulastumoralesdemama AT sosaldv efectosdemedioscondicionadosdefibroblastosestimuladosporcomponentesdietariossobrecelulastumoralesdemama AT ferrerov efectosdemedioscondicionadosdefibroblastosestimuladosporcomponentesdietariossobrecelulastumoralesdemama AT pasqualinime efectosdemedioscondicionadosdefibroblastosestimuladosporcomponentesdietariossobrecelulastumoralesdemama AT quintaraa efectosdemedioscondicionadosdefibroblastosestimuladosporcomponentesdietariossobrecelulastumoralesdemama AT sollaed a AT sosaldv a AT ferrerov a AT pasqualinime a AT quintaraa a |
| first_indexed |
2022-10-04T12:56:15Z |
| last_indexed |
2022-10-04T12:56:15Z |
| _version_ |
1770719112320778240 |
| spelling |
I10-R10-article-348502022-06-07T14:49:55Z Effects of conditioned media from fibroblasts stimulated by dietary components on breast tumoral cells Efectos de medios condicionados de fibroblastos estimulados por componentes dietarios sobre células tumorales de mama A Solla , ED Sosa, LDV Ferrero , V Pasqualini , ME Quintar , AA Carbohydrates fatty acids tumor microenvironment fibroblasts Carbohidratos ácidos grasos microambiente tumoral fibroblastos . Published evidence suggests a strong association between nutrition and the development and maintenance of tumoral processes. In Córdoba, a greater adherence to dietary patterns (DP) provided by carbohydrates- and saturated fatty acids-rich food, represented mainly by fructose (F) and palmitic acid (PA), is positively associated with the risk of colorectal and breast cancer. However, the cellular mechanisms by which these components promote tumor aggressiveness are not fully understood. Our objective was to evaluate in vitro the role of tumor-associated fibroblasts (CAFs) as mediators of the proliferative effects of F and PA. F88 cell line, corresponding to human breast cancer CAFs, was grown in DMEM with 10% FBS and stimulated with F 40 mM, AP 250 uM, combinations of both (F+PA) or their respective vehicles, for 24 hours (characteristic concentrations of DP in Cordoba). Subsequently, supernatants were removed, total proteins were quantified and stored at -80 °C for assays. Breast epithelial tumor cells of the MCF7 line were cultured in DMEM medium with 10% FBS and then stimulated with conditioned media (from F88 cells) for 24 hours. As controls, conditioned media from vehicle-treated cells were used. Any of the stimuli did not cause statistically significant changes in F88 cell proliferation. F + AP combination did induce a strong phenotypic change, with greater development of proteinopoietic and secretory organelles when observed by transmission electron microscopy. In addition, stimuli with PA and F + PA produced a decrease in the expression of Fibroblast Activation Protein (FAP) by western blot. On the other hand, MCF7 cells when stimulated with conditioned media from F88 treated with F + PA showed an increase in cell proliferation (ANOVA, p <0.05), determined by incorporation of bromodeoxyuridine and cell count. However, there were no significant differences between F and PA individually. These results suggest a pathogenic effect of food rich in F and PA on tumor proliferation in breast cancer, which would be mediated by CAFs. Numerosas evidencias señalan una fuerte asociación entre componentes nutricionales y el desarrollo y mantenimiento de procesos tumorales. En Córdoba, se describió que la mayor adherencia a patrones dietarios (PD) provistos por alimentos ricos en carbohidratos y ácidos grasos saturados, representados principalmente por fructosa (F) y ácido palmítico (AP) respectivamente, se asocia positivamente al riesgo de cáncer colorrectal y de mama. Sin embargo, no se conocen completamente los mecanismos celulares por los cuales estos componentes promoverían mayor agresividad tumoral. Nuestro objetivo fue evaluar in vitro el rol de los fibroblastos asociados a tumores (CAFs) como mediadores de posibles efectos proliferativos de F y AP. Para ello, células de la línea F88, correspondientes a CAFs de cáncer mamario humano fueron crecidas en medio DMEM con 10% SFB y estimuladas con F 40 mM, AP 250 uM, combinaciones de ambos (F+AP) o sus respectivos vehículos, por 24 horas (concentraciones características de PD cordobeses). Posteriormente, se retiraron sobrenadantes, se cuantificaron proteínas totales y se guardaron a -80 °C para los ensayos posteriores. Células tumorales epiteliales de mama de la línea MCF7 fueron cultivadas en medio DMEM con 10% SFB y posteriormente estimuladas con los medios condicionados (provenientes de las células F88) por 24 horas. Como controles, se utilizaron los medios condicionados de células tratadas con vehículo. Los estímulos no provocaron cambios estadísticamente significativos en la proliferación celular de las F88, aunque la combinación F+AP sí indujo un fuerte cambio fenotípico, con mayor desarrollo de organelas proteinopoiéticas y secretorias al ser observadas por microscopía electrónica de transmisión. Además, los estímulos con AP y F+AP produjeron una disminución en la expresión de Fibroblast Activation Protein (FAP) por western blot. Por otro lado, las células MCF7 al ser estimuladas con medios condicionados provenientes de F88 tratadas con F+AP mostraron un incremento en la proliferación celular (ANOVA, p<0,05), determinada mediante incorporación de bromodeoxiuridina y conteo celular. No obstante, no hubo diferencias significativas entre F y AP en forma individual. Estos resultados sugieren un efecto patogénico de alimentos ricos en F y AP sobre la proliferación tumoral en el cáncer de mama, que sería mediado por CAFs. . Universidad Nacional Cba. Facultad de Ciencias Médicas. Secretaria de Ciencia y Tecnología 2022-06-07 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion texto texto texto https://revistas.unc.edu.ar/index.php/med/article/view/34850 Revista de la Facultad de Ciencias Médicas de Córdoba.; Vol. 78 No. Suplemento (2021): Suplemento JIC XXII Revista de la Facultad de Ciencias Médicas de Córdoba; Vol. 78 Núm. Suplemento (2021): Suplemento JIC XXII Revista da Faculdade de Ciências Médicas de Córdoba; v. 78 n. Suplemento (2021): Suplemento JIC XXII 1853-0605 0014-6722 http://creativecommons.org/licenses/by-nc/4.0 |