Hight resolution optical microscopy of the cellular cycle in psoriasis patients.
Skin tissue from patients with Psoriasis was analyzed using HROM (High Resolution Optical Mlcroscopy), studying epithelial differentlation and posslble structural alterations of the queratinocytes. The samples were taken from 10 patients with histopathologlc diagnosis of Psoriasis. Thls tissue sampl...
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Universidad Nacional Cba. Facultad de Ciencias Médicas. Secretaria de Ciencia y Tecnología
2019
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| Acceso en línea: | https://revistas.unc.edu.ar/index.php/med/article/view/26112 |
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I10-R10-article-261122019-11-11T21:04:34Z Hight resolution optical microscopy of the cellular cycle in psoriasis patients. Estudio con microscopia optica de alta resolución (MOAR) del ciclo celular en pacientes con psoriasis. Frede, S Cabalier, MED Reinoso, N Berrotarán, N Juri, G Piccinni, DJ Asís, G Burgos, E Spitale, LS epithelial dlfferentlation in psoriasis high resolution optical microscopy (HROM) in psoriasis. diferenciación epitelial en psoriasis microscopía optica de alta resolución (MOAR) en psoriasis Skin tissue from patients with Psoriasis was analyzed using HROM (High Resolution Optical Mlcroscopy), studying epithelial differentlation and posslble structural alterations of the queratinocytes. The samples were taken from 10 patients with histopathologlc diagnosis of Psoriasis. Thls tissue samples where afflxed with glutaraldehide buffer- collidine for 48 hours. Later processed with the HROM technlque and colored with toluldine blue, metilene blue, basic Fuscine, and silver metenarnine. The basal epithelial elements presented ovoid nucleus and most of them had prominent nucleolus. In 7 of the studied cases, the granulose stratus was absent, and thinner in the rest, with nucleus and nucleolus retention. At thls level queratinocytes where observed with perinuclear anfofilia, as well as llnfocltic and macrophagic infiltrate and unlon complex where elongated. Antecedentes: La Psoriasis se caracteriza por incremento del ciclo celular a nivel epidérmico, evidenciado a nivel histopatológico por un intensa hipe rparaque ratos is, acantosis, papilomatosis e Inflamación crónica. Materiales y métodos: Se estudió con microscopia óptica de alta resolución (MOAR), la epidermis de pacientes portadores de soriasis, analizando la diferenciación epitelial y las posibles alteraciones estructurales de los queratinocitos. Resultados: Los especímenes correspondieron a 10 pacientes con diagnóstico clínico e histopatológico de psoriasis. Las biopsias de piel fueron fijadas en solución de glutaraldehído buffercollidina durante 48 hs, procesados con la técnica para MOARy coloreados con azul de toluidina, azul de metileno, fuscina básica y metenamina- plata. Los elementos epiteliales basales presentaban núcleos ovoideos, varios de ellos con nucleólos prominentes. En 7 de los casos estudiados el estrato granuloso estuvo ausente y en los 3 casos restantes observamos retención de núcleos y nucleólos. Conclusiones: Se observaron queratinocitos con anfofilla perinuclear, complejos de unión elongados e Infiltrado linfocitario y de macrofagos. Universidad Nacional Cba. Facultad de Ciencias Médicas. Secretaria de Ciencia y Tecnología 2019-11-06 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf https://revistas.unc.edu.ar/index.php/med/article/view/26112 Revista de la Facultad de Ciencias Médicas de Córdoba.; Vol. 64 No. 1 (2007); 18 - 23 Revista de la Facultad de Ciencias Médicas de Córdoba; Vol. 64 Núm. 1 (2007); 18 - 23 Revista da Faculdade de Ciências Médicas de Córdoba; v. 64 n. 1 (2007); 18 - 23 1853-0605 0014-6722 spa https://revistas.unc.edu.ar/index.php/med/article/view/26112/27942 Derechos de autor 2019 Universidad Nacional de Córdoba |
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Universidad Nacional de Córdoba |
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I-10 |
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R-10 |
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Revistas de la UNC |
| language |
Español |
| format |
Artículo revista |
| topic |
epithelial dlfferentlation in psoriasis high resolution optical microscopy (HROM) in psoriasis. diferenciación epitelial en psoriasis microscopía optica de alta resolución (MOAR) en psoriasis |
| spellingShingle |
epithelial dlfferentlation in psoriasis high resolution optical microscopy (HROM) in psoriasis. diferenciación epitelial en psoriasis microscopía optica de alta resolución (MOAR) en psoriasis Frede, S Cabalier, MED Reinoso, N Berrotarán, N Juri, G Piccinni, DJ Asís, G Burgos, E Spitale, LS Hight resolution optical microscopy of the cellular cycle in psoriasis patients. |
| topic_facet |
epithelial dlfferentlation in psoriasis high resolution optical microscopy (HROM) in psoriasis. diferenciación epitelial en psoriasis microscopía optica de alta resolución (MOAR) en psoriasis |
| author |
Frede, S Cabalier, MED Reinoso, N Berrotarán, N Juri, G Piccinni, DJ Asís, G Burgos, E Spitale, LS |
| author_facet |
Frede, S Cabalier, MED Reinoso, N Berrotarán, N Juri, G Piccinni, DJ Asís, G Burgos, E Spitale, LS |
| author_sort |
Frede, S |
| title |
Hight resolution optical microscopy of the cellular cycle in psoriasis patients. |
| title_short |
Hight resolution optical microscopy of the cellular cycle in psoriasis patients. |
| title_full |
Hight resolution optical microscopy of the cellular cycle in psoriasis patients. |
| title_fullStr |
Hight resolution optical microscopy of the cellular cycle in psoriasis patients. |
| title_full_unstemmed |
Hight resolution optical microscopy of the cellular cycle in psoriasis patients. |
| title_sort |
hight resolution optical microscopy of the cellular cycle in psoriasis patients. |
| description |
Skin tissue from patients with Psoriasis was analyzed using HROM (High Resolution Optical Mlcroscopy), studying epithelial differentlation and posslble structural alterations of the queratinocytes. The samples were taken from 10 patients with histopathologlc diagnosis of Psoriasis. Thls tissue samples where afflxed with glutaraldehide buffer- collidine for 48 hours. Later processed with the HROM technlque and colored with
toluldine blue, metilene blue, basic Fuscine, and silver metenarnine. The basal epithelial elements presented ovoid nucleus and most of them had prominent nucleolus. In 7 of the studied cases, the granulose stratus was absent, and thinner in the rest, with nucleus and nucleolus retention. At thls level queratinocytes where observed with perinuclear anfofilia, as well as llnfocltic and macrophagic infiltrate and unlon complex where elongated. |
| publisher |
Universidad Nacional Cba. Facultad de Ciencias Médicas. Secretaria de Ciencia y Tecnología |
| publishDate |
2019 |
| url |
https://revistas.unc.edu.ar/index.php/med/article/view/26112 |
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