First evidence of Bnyamwera orthobunyavirus circulation in Formosa province, Argentina
Bunyamwera orthobunyavirus (BUNV) virus is an arbovirus kept in nature in a cycle that mainly involves mosquitoes and vertebrates. In America, this virus is important because of its potential pathogenicity for both human and domestic animals. In Argentina, Aedes (Ochlerotatus) albifasciatus (Córdoba...
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| Formato: | Artículo revista |
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Universidad Nacional Cba. Facultad de Ciencias Médicas. Secretaria de Ciencia y Tecnología
2019
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| Acceso en línea: | https://revistas.unc.edu.ar/index.php/med/article/view/25755 |
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I10-R10-article-25755 |
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ojs |
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Universidad Nacional de Córdoba |
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I-10 |
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R-10 |
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Revistas de la UNC |
| language |
Español |
| format |
Artículo revista |
| topic |
Bunyamwera Orthobunyavirus virus Arbovirus Mosquitoes Formosa Virus Bunyamwera Orthobunyavirus Arbovirus Mosquitos Formosa |
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Bunyamwera Orthobunyavirus virus Arbovirus Mosquitoes Formosa Virus Bunyamwera Orthobunyavirus Arbovirus Mosquitos Formosa Gallardo , R Albrieu , G Laurito, M Mansilla, A Beranek, M Contigiani, M Spinsanti , L First evidence of Bnyamwera orthobunyavirus circulation in Formosa province, Argentina |
| topic_facet |
Bunyamwera Orthobunyavirus virus Arbovirus Mosquitoes Formosa Virus Bunyamwera Orthobunyavirus Arbovirus Mosquitos Formosa |
| author |
Gallardo , R Albrieu , G Laurito, M Mansilla, A Beranek, M Contigiani, M Spinsanti , L |
| author_facet |
Gallardo , R Albrieu , G Laurito, M Mansilla, A Beranek, M Contigiani, M Spinsanti , L |
| author_sort |
Gallardo , R |
| title |
First evidence of Bnyamwera orthobunyavirus circulation in Formosa province, Argentina |
| title_short |
First evidence of Bnyamwera orthobunyavirus circulation in Formosa province, Argentina |
| title_full |
First evidence of Bnyamwera orthobunyavirus circulation in Formosa province, Argentina |
| title_fullStr |
First evidence of Bnyamwera orthobunyavirus circulation in Formosa province, Argentina |
| title_full_unstemmed |
First evidence of Bnyamwera orthobunyavirus circulation in Formosa province, Argentina |
| title_sort |
first evidence of bnyamwera orthobunyavirus circulation in formosa province, argentina |
| description |
Bunyamwera orthobunyavirus (BUNV) virus is an arbovirus kept in nature in a cycle that mainly involves mosquitoes and vertebrates. In America, this virus is important because of its potential pathogenicity for both human and domestic animals. In Argentina, Aedes (Ochlerotatus) albifasciatus (Córdoba) and Psorospora (Grabhamia) varinervis (Santa Fe) mosquito strains have been recovered; and from horses with neurological syndrome and an equine abortion (Santa Fe). Between 2008-2009 and 2013-2014, mosquitoes of other species infected by BUNV were detected in the Center and Northeast of the province of Córdoba, their involvement in public health being unknown. In Córdoba, the presence of neutralizing antibodies in humans was demonstrated, and a clinical case was confirmed by BUNV with febrile syndrome reported as Dengue during the outbreak that produced this virus in 2009.
The objective of this work was to know the presence / absence of BUNV in mosquitoes in the town of San Francisco Laishí in Formosa province during the autumn of 2017.
We analyzed 21 pools, each consisting of 50 individuals per species of mosquitoes, previously identified and collected in a field in the San Francisco Laishí. BUNV was detected by generic RT-PCR for orthobunyavirus that amplifies a region of 251 (base pairs) of genomic segment S and subsequent sequencing and phylogenetic analysis.
Of the total pools analyzed, 6 were obtained with a positive result. The positive pools corresponded to the species Culex (Melanoconion) educator (2), Cx (Mel) bastagarius, Cx (Mel) intrincatus, Ae (Och) Sp, Cx (cux) acharistus. Five samples were sequenced, which were grouped together with those previously determined in the same clade.
These preliminary results, experienced a contribution to knowledge about the circulation of the Orthobunyavirus genus in Argentina and encourage us to continue research regarding the role of BUNV as a human and / or domestic animal pathogen and its maintenance cycle. |
| publisher |
Universidad Nacional Cba. Facultad de Ciencias Médicas. Secretaria de Ciencia y Tecnología |
| publishDate |
2019 |
| url |
https://revistas.unc.edu.ar/index.php/med/article/view/25755 |
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I10-R10-article-257552019-11-11T21:18:27Z First evidence of Bnyamwera orthobunyavirus circulation in Formosa province, Argentina Primera evidencia de circulación de virus Bnyamwera orthobunyvirus en la provincia de Formosa Gallardo , R Albrieu , G Laurito, M Mansilla, A Beranek, M Contigiani, M Spinsanti , L Bunyamwera Orthobunyavirus virus Arbovirus Mosquitoes Formosa Virus Bunyamwera Orthobunyavirus Arbovirus Mosquitos Formosa Bunyamwera orthobunyavirus (BUNV) virus is an arbovirus kept in nature in a cycle that mainly involves mosquitoes and vertebrates. In America, this virus is important because of its potential pathogenicity for both human and domestic animals. In Argentina, Aedes (Ochlerotatus) albifasciatus (Córdoba) and Psorospora (Grabhamia) varinervis (Santa Fe) mosquito strains have been recovered; and from horses with neurological syndrome and an equine abortion (Santa Fe). Between 2008-2009 and 2013-2014, mosquitoes of other species infected by BUNV were detected in the Center and Northeast of the province of Córdoba, their involvement in public health being unknown. In Córdoba, the presence of neutralizing antibodies in humans was demonstrated, and a clinical case was confirmed by BUNV with febrile syndrome reported as Dengue during the outbreak that produced this virus in 2009. The objective of this work was to know the presence / absence of BUNV in mosquitoes in the town of San Francisco Laishí in Formosa province during the autumn of 2017. We analyzed 21 pools, each consisting of 50 individuals per species of mosquitoes, previously identified and collected in a field in the San Francisco Laishí. BUNV was detected by generic RT-PCR for orthobunyavirus that amplifies a region of 251 (base pairs) of genomic segment S and subsequent sequencing and phylogenetic analysis. Of the total pools analyzed, 6 were obtained with a positive result. The positive pools corresponded to the species Culex (Melanoconion) educator (2), Cx (Mel) bastagarius, Cx (Mel) intrincatus, Ae (Och) Sp, Cx (cux) acharistus. Five samples were sequenced, which were grouped together with those previously determined in the same clade. These preliminary results, experienced a contribution to knowledge about the circulation of the Orthobunyavirus genus in Argentina and encourage us to continue research regarding the role of BUNV as a human and / or domestic animal pathogen and its maintenance cycle. El virus Bunyamwera orthobunyavirus (BUNV) es un arbovirus mantenido en la naturaleza en un ciclo que involucra principalmente a mosquitos y a vertebrados. En América, este virus cobra importancia por su potencial patogenicidad tanto para animales domésticos como para humanos. En Argentina se han recuperado cepas de mosquitos Aedes (Ochlerotatus) albifasciatus (Córdoba) y Psorospora (Grabhamia) varinervis (Santa Fe); y a partir de caballos con síndrome neurológico y de un aborto equino (Santa Fe). Entre los años 2008-2009 Y 2013-2014 se detectaron mosquitos de otras especies infectados por BUNV en el Centro y Noreste de la provincia de Córdoba, desconociéndose su implicancia en la salud pública. En Córdoba se demostró la presencia de anticuerpos neutralizantes en humanos, y se confirmó un caso clínico por BUNV con síndrome febril notificado como Dengue durante el brote que produjo este virus en el año 2009. El objetivo de este trabajo fue conocer la presencia/ausencia de BUNV en mosquitos de la localidad de San Francisco Laishí de la provincia de Formosa durante el otoño del 2017. Se analizaron 21 pools cada uno conformado con 50 individuos po especie de mosquitos, previamente identificados y colectados en un campo de la localidad San Francisco Laishí. Se realizó detección de BUNV mediante RT-PCR genérica para Orthobunyavirus que amplifica una región de 251 (pares de bases) del segmento genómico S y posterior secuenciación y análisis filogenético. Del total de pools analizados, se obtuvieron 6 con resultado positivo. Los pools positivos correspondieron a las especies Culex (Melanoconion) educator (2), Cx (Mel) bastagarius, Cx (Mel) intrincatus, Ae (Och) Sp, Cx (cux) acharistus. Cinco muestras pudieron ser secuenciadas, las cuales agruparon junto con las anteriormente aisladas en el mismo clado. Estos resultados preliminares, constituyen un aporte al conocimiento sobre la circulación del género Orthobunyavirus en Argentina y nos animan a continuar las investigaciones respecto al rol de BUNV como patógeno humano y/o de animales domésticos y sobre su ciclo de mantenimiento. Universidad Nacional Cba. Facultad de Ciencias Médicas. Secretaria de Ciencia y Tecnología 2019-10-15 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf https://revistas.unc.edu.ar/index.php/med/article/view/25755 Revista de la Facultad de Ciencias Médicas de Córdoba.; 2019: Suplemento JIC XX Revista de la Facultad de Ciencias Médicas de Córdoba; 2019: Suplemento JIC XX Revista da Faculdade de Ciências Médicas de Córdoba; 2019: Suplemento JIC XX 1853-0605 0014-6722 spa https://revistas.unc.edu.ar/index.php/med/article/view/25755/27430 Derechos de autor 2019 Universidad Nacional de Córdoba |