Pectinolytic enzyme production by Colletotrichum truncatum, causal agent of soybean anthracnose
Background: Colletotrichum truncatum is the most common pathogenic fungus associated with soybean anthracnose, a prevalent disease in Argentina. Pectinolytic enzymes are involved in the pathogenicity of a wide range of plant pathogenic fungi. Objectives: To explore pectinolytic enzyme production in...
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| Formato: | Capítulo de libro |
| Lenguaje: | Español |
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2010
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| Acceso en línea: | Registro en Scopus DOI Handle Registro en la Biblioteca Digital |
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| Sumario: | Background: Colletotrichum truncatum is the most common pathogenic fungus associated with soybean anthracnose, a prevalent disease in Argentina. Pectinolytic enzymes are involved in the pathogenicity of a wide range of plant pathogenic fungi. Objectives: To explore pectinolytic enzyme production in Argentinian Colletotrichum strains isolated from diseased soybean plants from different geographic locations, as a preliminary step to establish the biological role of the pectinolytic enzymes in the Colletotrichum spp.-soybean system, yet unknown. Methods: Ten strains were screened for in vitro pectinolytic enzyme production on a defined medium based on pectin as carbon source. Results: All isolates were able to grow in this medium and polymethylgalacturonase (PMG), polygalacturonase (PG) and pectin lyase (PL) activities were detected. On the whole, the peak of polygalacturonases activities preceded the day of maximum growth, while PL activity reached its highest level afterwards. Strain BAFC 3097 (from Santa Fe province) yielded high titles of the three enzymes (1.08. U/ml PG, 1.05. U/ml PMG, 156. U/ml PL), after a short incubation period (7-10 days). Low synthesis of polygalacturonases in cultures containing glucose as unique carbon source suggests that these enzymes are constitutive in contrast with PL, which was not detected. Conclusions: The disparity observed in enzyme production among strains cannot be related to fungal growth, since no major differences in mycelial yield were found; it was not connected with their geographic origin, but might be associated with differences in virulence among strains not yet evaluated. © 2010 Revista Iberoamericana de Micología. |
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| Bibliografía: | Armstrong-Cho, C.L., Banniza, S., Glomerella truncata sp. nov., the teleomorph of Colletotrichum truncatum (2006) Mycol Res, 110, pp. 951-956 Bailey, J.A., O'Connell, R.J., Pring, R.J., Nash, C., Infection strategies of Colletotrichum species (1992) Colletotrichum: biology, pathology and control, pp. 88-120. , CAB International, Wallingford, UK, J.A. Bailey, M.J. Jeger (Eds.) Biely, P., Heinrichová, K., Kruziková, M., Induction and inducers of the pectolytic system in Aerobasidium pullulans (1996) Curr Microbiol, 33, pp. 6-10 Blais, P., Rogers, P.A., Charest, P.M., Kinetics of the production of polygalacturonase and pectin lyase by two closely related Formae speciales of Fusarium oxysporum (1992) Exp Mycol, 16, pp. 1-7 Centis, S., Guillas, I., Séjalon, N., Esquerré-Tugayé, Dumas, B., Endopolygalacturonase genes from Colletotrichum lindemuthianum: cloning of CLPG 2 and comparison of its expression to that of CLPG1 during saprophytic and parasitic growth of the fungus (1997) Mol Plant-Microbe Interact, 10, pp. 769-775 Chacko, S., Khare, M.N., Agrawal, S.C., Cellulolytic and pectinolytic enzyme production by Colletotrichum dematium f. sp. truncata causing anthracnose of soybeans (1978) Indian Phytopathol, 31, pp. 69-72 Collmer, A., Keen, N., The role of the pectic enzymes in plant pathogenesis (1986) Annu Rev Phytopathol, 24, pp. 383-409 Cooper, R.M., Wood, R.K.S., Regulation of synthesis of cell-wall degrading enzymes by Verticillium alboatum and Fusarium oxysporum f. sp. lycopersici (1975) Physiol Plant Pathol, 5, pp. 135-156 Drori, N., Kramer-Haimovich, H., Rollins, J., Dinoor, A., Okon, Y., Pines, O., External pH and nitrogen source affect secretion of pectate lyase by Colletotrichum gloeosporioides (2003) Appl Environ Microb, 69, pp. 3258-3262 Durrands, P.K., Cooper, R.M., The role of pectinases in vascular wilt disease as determined by defined mutants of Verticillum albo-altrum (1988) Physiol Mol Plant Pathol, 32, pp. 363-371 Fernando, T.H.P.S., Jayasinghe, C.K., Wijesundera, R.L.C., Cell wall degrading enzyme secretion by Colletotrichum acutatum, the causative fungus of secondary leaf all of Hevea basiliensis (rubber) (2001) Mycol Res, 105, pp. 195-201 Forseille, L., Molecular and pathological differentiation of Colletotrichum truncatum from scentless chamomile and legume crops. Master's thesis (2007) Saskatoon (Sask): University of Saskatchewan., p. 122 Fraissinet-Tachet, L., Fevre, M., Regulation by galacturonic acid of pectinolytic enzyme production by Sclerotinia sclerotiorum (1996) Curr Microbiol, 33, pp. 49-53 Guevara, M.A., González-Jaen, M.T., Estevez, P., Multiple forms of pectic lyases and polygalacturonase from Fusarium oxysporum f. sp. radicis lycopersici: regulation of their synthesis by galacturonic acid (1997) Can J Microbiol, 43, pp. 245-253 Herbert, C., O'Connell, R., Gaulin, E., Salesses, V., Esquerré-Tugayé, M.T., Dumas, B., Production of a cell wall-associated endopolygalacturonase by Colletotrichum lindemuthianum and pectin degradation during bean infection (2004) Fungal Genet Biol, 41, pp. 140-147 Hernández-Silva, L., Piñón Escobedo, C., Cano-Camacho, H., Zavala-Páramo, M.G., Acosta-Rodríguez, I., López-Romero, E., Comparison of fungal growth and production of extracellular pectin lyase activity by pathogenic and non-pathogenic races of Colletotrichum lindemuthianum cultivated under different conditions (2007) Physiol Mol Plant Pathol, 70, pp. 88-95 Hugouvieux, V., Centis, S., Lafitte, C., Esquerre-Tugaye, M.-T., Induction by a-L-arabinose and a-L-rhamnose of endopolygalacturonase gene expression in Colletotrichum lindemuthianum (1997) Appl Environ Microbiol, 63, pp. 2287-2292 Hyde, K.D., Cai, L., Cannon, P.F., Crouch, J.A., Crous, P.W., Damm, U., Colletotrichum-names in current use (2009) Fungal Div, 39, pp. 147-182 Idnurm, A., Howlett, B.J., Pathogenicity genes of phyto-pathogenic fungi (2001) Mol Plant Pathol, 2, pp. 241-255 Jayasinghe, C.K., Wijayaratne, S.C.P., Fernando, T.H.P.S., Characterization of cell wall degrading enzymes of Thanatephorus cucumeris (2004) Mycopathologia, 157, pp. 73-79 Kapat, A., Zimand, Y., Elad, Y., Biosynthesis of pathogenicity hydrolytic enzymes by Botrytis cinerea during infection of bean leaves and in vitro (1998) Mycol Res, 102, pp. 1017-1024 Latunde -Dada, A.O., O'Connell, R.J., Nash, C., Pring, R.J., Lucas, J.A., Bailey, J.A., Infection process and identity of the hemibiotrophic fungus Colletotrichum destructivum (O'Gara) from cowpea (Vigna unguiculata (L) Walp) (1996) Mycol Res, 100, pp. 1133-1141 Lisker, N., Katan, J., Henis, Y., Sequential production of polygalacturonase, cellulose and pectin lyase by Rhizoctonia solani (1975) Can J Microbiol, 21, pp. 1298-1304 Manandhar, J.B., Kunwar, I.K., Singh, T., Hartman, G.L., Sinclair, J.B., Penetration and infection of soybean leaf tissues by Colletotrichum truncatum and Glomerella glycines (1985) Phytopathology, 75, pp. 704-708 Martínez, M.J., Martínez, R., Reyes, F., Effect of pectin on pectinases in autolysis of Botrytis cinerea (1988) Mycopathologia, 102, pp. 37-43 Martínez, M.J., Alconada, M.T., Guillén, F., Vázquez, C., Reyes, F., Pectic activities from Fusarium oxysporum f. sp. melonis: purification and characterization of an exopolygalacturonase (1991) FEMS Microbiol Lett, 81, pp. 145-150 O'Connell, R.J., Bailey, J.A., Richmond, D.V., Cytology and physiology of infection of Phaseolus vulgaris by Colletotrichum lindemuthianum (1985) Physiol Plant Pathol, 27, pp. 75-98 O'Connell, R.J., Bailey, J.A., Hemibiotrophy in Colletotrichum lindemuthianum (1991) Electron microscopy of plant pathogens, pp. 211-222. , Springer-Verlag, Berlin, Germany, K. Mendgen, D.E. Lesemann (Eds.) O'Connell, R.J., Uronu, A.B., Waksman, G., Nash, C., Keon, J.P.R., Bailey, J.A., Hemibiotrophic infection of Pisum sativum by Colletotrichum truncatum (1993) Plant Pathol, 42, pp. 774-783 Olutiola, P.O., Akintunde, O.A., Pectin lyase and pectin methyl esterase production by Penicillium citrinum (1979) Trans Br Mycol Soc, 72, pp. 49-55 Perfect, S., Bleddyn Hughes, H., O'Connell, R., Green, J.R., Colletotrichum: a model genus for studies on pathology and fungal-plant interactions (1999) Fungal Genet Biol, 27, pp. 186-198 Prusky, D., McEvoy, J.L., Leverentz, B., Local modulation of host pH by Colletotrichum species as a mechanism to increase virulence (2001) Mol Plant-Microbe Interact, 14, pp. 1105-1113 Ramos, A.M., Cinto, I.E., Franco Tadic, L., Carmona, M., Gally, M., Caracterización molecular de especies de Colletotrichum causantes de antracnosis en soja (Glycine max) (2009) Bol Soc Argent Bot, 44, p. 120 Riou, C.H., Freyssinet, G., Fevre, M., Production of cell wall-degrading enzymes by the phytopathogenic fungus Sclerotinia sclerotiorum (1991) Appl Environ Microbiol, 57, pp. 1478-1484 Rogers, L.M., Kim, Y.K., Guo, W., González Carranza, L., Li, D., Kolattukudy, P.E., Requirement for either a host or pectin-induced pectate lyase for infection of Pisum sativum by Nectria hematococca (2000) Proc Natl Acad Sci, 97, pp. 9813-9818 Sakal, T., Sakamoto, T., Hallaert, J., Vandamme, E.J., Pectin, pectinase and protopectinase: production, properties and applications (1995) Adv Appl Microbiol, 39, pp. 2213-2293 Sinclair, J.B., Backman, P.A., (1982) Compendium of soybean diseases, , The American Phytopathological Society, St. Paul, USA Solís-Pereira, S., Favela-Torres, E., Viniegra-González, G., Gutiérrez-Rojas, M., Effects of different carbon sources on the synthesis of pectinase by Aspergillus niger in submerged and solid state fermentations (1993) Appl Microbiol Biotechnol, 39, pp. 36-41 Somogy, M.J., Notes on sugar determination (1952) Biol Chem, 159, pp. 19-23 Sutton, B.C., The genus Glomerella and its anamorph Colletotrichum (1992) Colletotrichum: biology, pathology and control, pp. 1-26. , CAB International, Wallingford, UK, J.A. Bailey, M.J. Leger (Eds.) Szajer, I., Szajer, C., Formation and release of pectin lyase during growth of Penicillium paxilli (1985) Biotechnol Lett, 7, pp. 105-108 Vázquez, C., Reyes, F., Martínez, M.J., Comparative study of pectic activities from different Formae speciales of Fusarium oxysporum (1993) Lett Appl Microbiol, 16, pp. 210-213 Wattad, C., Kobiler, D., Dinoor, A., Prusky, D., Pectate lyase of Colletotrichum gloeosporioides attacking avocado fruits: cDNA cloning and involvement in pathogenicity (1997) Physiol Mol Plant Pathol, 50, pp. 197-212 Wijesundera, R.L., Bailey, J.A., Byrde, R.J., Production of pectin lyase by Colletotrichum lindemuthianum in culture and in infected bean (Phaseolus vulgaris) tissue (1984) J Gen Microbiol, 130, pp. 285-290 Wijesundera, R.L., Bailey, J.A., Byrde, R.J., Fielding, A.H., Cell wall degrading enzymes of Colletotrichum lindemuthianum: their role in the development of bean anthracnose (1989) Physiol Mol Plant Pathol, 34, pp. 403-413 Wrather, J.A., Anderson, T.R., Arsyad, D.M., Tan, Y., Ploper, L.D., Porta-Puglia, A., Soybean disease loss estimates for the top 10 soybean producing countries in 1998 (2001) Can J Plant Pathol, 23, pp. 122-131 |
| ISSN: | 11301406 |
| DOI: | 10.1016/j.riam.2010.06.002 |