An arsenic fluorescent compound as a novel probe to study arsenic-binding proteins
Arsenic-binding proteins are under continuous research. Their identification and the elucidation of arsenic/ protein interaction mechanisms are important because the biological effects of these complexes may be related not only to arsenic but also to the arsenic/protein structure. Although many prot...
Guardado en:
| Autor principal: | |
|---|---|
| Otros Autores: | , , , , , |
| Formato: | Capítulo de libro |
| Lenguaje: | Inglés |
| Publicado: |
2012
|
| Acceso en línea: | Registro en Scopus DOI Handle Registro en la Biblioteca Digital |
| Aporte de: | Registro referencial: Solicitar el recurso aquí |
| LEADER | 12733caa a22015257a 4500 | ||
|---|---|---|---|
| 001 | PAPER-23301 | ||
| 003 | AR-BaUEN | ||
| 005 | 20230518205457.0 | ||
| 008 | 190411s2012 xx ||||fo|||| 00| 0 eng|d | ||
| 024 | 7 | |2 scopus |a 2-s2.0-84871453073 | |
| 024 | 7 | |2 cas |a arsenic, 7440-38-2; fluorescein isothiocyanate, 25168-13-2, 27072-45-3, 3326-32-7; Arsenic, 7440-38-2; Arsenicals; Carrier Proteins; Fluorescein-5-isothiocyanate, 3326-32-7; Fluorescent Dyes; Thioredoxins, 52500-60-4; p-aminophenylarsine oxide | |
| 040 | |a Scopus |b spa |c AR-BaUEN |d AR-BaUEN | ||
| 030 | |a PJROA | ||
| 100 | 1 | |a Femia, A.L. | |
| 245 | 1 | 3 | |a An arsenic fluorescent compound as a novel probe to study arsenic-binding proteins |
| 260 | |c 2012 | ||
| 270 | 1 | 0 | |m Del Alonso, S.V.; Laboratorio de Biomembranas, Departamento de Ciencia y Tecnología, Universidad Nacional de Quilmes, Roque Saenz Peña 352, Bernal, B1876BXD Buenos Aires, Argentina; email: salonso@unq.edu.ar |
| 506 | |2 openaire |e Política editorial | ||
| 504 | |a Ado, K., Taniguchi, Y., (2007) Biochim Biophys Acta, 1774 (7), pp. 813-821 | ||
| 504 | |a Ansorge, W., Sproat, B., Stegemann, J., Schwager, C., Zenke, M., (1987) Nucleic Acids Res, 15 (11), pp. 4593-4602 | ||
| 504 | |a Ansorge, W., Rosenthal, A., Sproat, B., Schwager, C., Stegemann, J., Voss, H., (1988) Nucleic Acids Res, 16 (5), pp. 2203-2206 | ||
| 504 | |a Berry, F.B., Skarie, J.M., Mirzayans, F., Fortin, Y., Hudson, T.J., Raymond, V., Link, B.A., Walter, M.A., (2008) Hum Mol Genet, 17 (4), pp. 490-505 | ||
| 504 | |a Brown, C.J., Dastidar, S.G., See, H.Y., Coomber, D.W., Ortiz-Lombardia, M., Verma, C., Lane, D.P., (2010) J Mol Biol, 395 (4), pp. 871-883 | ||
| 504 | |a Burwell, L.S., Nadtochiy, S.M., Tompkins, A.J., Young, S., Brookes, P.S., (2006) Biochem J, 394 (PART 3), pp. 627-634 | ||
| 504 | |a Carbajal, M.L., Espinoza, S.L.S., Valdez, S.N., Poskus, E., Smolko, E.E., Grasselli, M., (2009) React Funct Polym, 69 (11), pp. 816-820 | ||
| 504 | |a Cleland, W.W., (1964) Biochemistry, 3, pp. 480-482 | ||
| 504 | |a Clerico, E.M., Ermacora, M.R., (2001) Arch Biochem Biophys, 395 (2), pp. 215-224 | ||
| 504 | |a Cline, D.J., Thorpe, C., Schneider, J.P., (2003) J Am Chem Soc, 125 (10), pp. 2923-2929 | ||
| 504 | |a Collet, J.F., Messens, J., (2010) Antioxid Redox Signal, 13 (8), pp. 1205-1216 | ||
| 504 | |a Coto-Garcia, A.M., Sotelo-Gonzalez, E., Fernandez-Arguelles, M.T., Pereiro, R., Costa-Fernandez, J.M., Sanz-Medel, A., (2011) Anal Bioanal Chem, 399 (1), pp. 29-42 | ||
| 504 | |a Chen, Y., Parr, T., Holmes, A.E., Nakanishi, K., (2008) Bioconjug Chem, 19 (1), pp. 5-9 | ||
| 504 | |a Donoghue, N., Yam, P.T., Jiang, X.M., Hogg, P.J., (2000) Protein Sci, 9 (12), pp. 2436-2445 | ||
| 504 | |a Dorward, A., Sweet, S., Moorehead, R., Singh, G., (1997) J Bioenerg Biomembr, 29 (4), pp. 385-392 | ||
| 504 | |a Edgington, L.E., Berger, A.B., Blum, G., Albrow, V.E., Paulick, M.G., Lineberry, N., Bogyo, M., (2009) Nat Med, 15 (8), pp. 967-973 | ||
| 504 | |a Femia, A.L., Temprana, C.F., Amor, M.S., Grasselli, M., Alonso Sdel, V., (2012) Med Chem, 8 (2), pp. 222-229 | ||
| 504 | |a Fernando, M.R., Nanri, H., Yoshitake, S., Nagata-Kuno, K., Minakami, S., (1992) Eur J Biochem/FEBS, 209 (3), pp. 917-922 | ||
| 504 | |a Flora, S.J., (2011) Free Radic Biol Med, 51 (2), pp. 257-281 | ||
| 504 | |a Fu, G.H., Wang, Y., Xi, Y.H., Guo, Z.W., Liu, X.B., Bai, S.Z., Yang, B.F., Chen, G.Q., (2005) J Drug Target, 13 (4), pp. 235-243 | ||
| 504 | |a Ge, Y., Qi, Z., Wang, Y., Liu, X., Li, J., Xu, J., Liu, J., Shen, J., (2009) Int J Biochem Cell Biol, 41 (4), pp. 900-906 | ||
| 504 | |a Gholivand, M.B., Ghasemi, J.B., Saaidpour, S., Mohajeri, A., (2008) Spectrochim Acta A Mol Biomol Spectrosc, 71 (3), pp. 1158-1165 | ||
| 504 | |a Gorman, J.J., (1987) Anal Biochem, 160 (2), pp. 376-387 | ||
| 504 | |a Griffin, B.A., Adams, S.R., Jones, J., Tsien, R.Y., (2000) Methods Enzymol, 327, pp. 565-578 | ||
| 504 | |a Gupta, S., Yel, L., Kim, D., Kim, C., Chiplunkar, S., Gollapudi, S., (2003) Mol Cancer Ther, 2 (8), pp. 711-719 | ||
| 504 | |a Holmgren, A., (1979) J Biol Chem, 254 (19), pp. 9627-9632 | ||
| 504 | |a Holmgren, A., (1995) Structure, 3 (3), pp. 239-243 | ||
| 504 | |a Jeng, M.F., Campbell, A.P., Begley, T., Holmgren, A., Case, D.A., Wright, P.E., Dyson, H.J., (1994) Structure, 2 (9), pp. 853-868 | ||
| 504 | |a Kitchin, K.T., Wallace, K., (2006) J Biochem Mol Toxicol, 20 (1), pp. 48-56 | ||
| 504 | |a Kitchin, K.T., Wallace, K., (2008) J Inorg Biochem, 102 (3), pp. 532-539 | ||
| 504 | |a Laurent, T.C., Moore, E.C., Reichard, P., (1964) J Biol Chem, 239, pp. 3436-3444 | ||
| 504 | |a Layton, C.J., Hellinga, H.W., (2010) Biochemistry, 49 (51), pp. 10831-10841 | ||
| 504 | |a Lundstrom, J., Krause, G., Holmgren, A., (1992) J Biol Chem, 267 (13), pp. 9047-9052 | ||
| 504 | |a Martin, S.R., Schilstra, M.J., (2008) Methods Cell Biol, 84, pp. 263-293 | ||
| 504 | |a Miller Jr., W.H., Schipper, H.M., Lee, J.S., Singer, J., Waxman, S., (2002) Cancer Res, 62 (14), pp. 3893-3903 | ||
| 504 | |a Mitsui, A., Hirakawa, T., Yodoi, J., (1992) Biochem Biophys Res Commun, 186 (3), pp. 1220-1226 | ||
| 504 | |a Nakamura, H., Masutani, H., Yodoi, J., (2006) Semin Cancer Biol, 16 (6), pp. 444-451 | ||
| 504 | |a Nakamura, H., Hoshino, Y., Okuyama, H., Matsuo, Y., Yodoi, J., (2009) Adv Drug Deliv Rev, 61 (4), pp. 303-309 | ||
| 504 | |a Oblong, J.E., Berggren, M., Powis, G., (1994) FEBS Lett, 343 (1), pp. 81-84 | ||
| 504 | |a Olson, E.S., Jiang, T., Aguilera, T.A., Nguyen, Q.T., Ellies, L.G., Scadeng, M., Tsien, R.Y., (2010) Proc Natl Acad Sci USA, pp. 1-6 | ||
| 504 | |a Palaniappan, P.R., Vijayasundaram, V., (2008) Food Chem Toxicol, 46 (11), pp. 3534-3539 | ||
| 504 | |a Ralph, S.J., (2008) Metal Based Drugs, 2008, p. 260146 | ||
| 504 | |a Ramadan, D., Cline, D.J., Bai, S., Thorpe, C., Schneider, J.P., (2007) J Am Chem Soc, 129 (10), pp. 2981-2988 | ||
| 504 | |a Ramadan, D., Rancy, P.C., Nagarkar, R.P., Schneider, J.P., Thorpe, C., (2009) Biochemistry, 48 (2), pp. 424-432 | ||
| 504 | |a Santos, J., Marino-Buslje, C., Kleinman, C., Ermacora, M.R., Delfino, J.M., (2007) Biochemistry, 46 (17), pp. 5148-5159 | ||
| 504 | |a Savickiene, J., Treigyte, G., Gineitis, A., Navakauskiene, R., (2010) Vitro Cell Dev Biol Anim, 46 (6), pp. 547-559 | ||
| 504 | |a Sebastia, J., Cristofol, R., Martin, M., Rodriguez-Farre, E., Sanfeliu, C., (2003) Cytometry A J Int Soc Anal Cytol, 51 (1), pp. 16-25 | ||
| 504 | |a Singh, B.R., Infrared analysis of peptides and proteins: Principles and applications (2000) ACS Symposium Series, 750. , American Chemical Society, Washington | ||
| 504 | |a Stevenson, K.J., Hale, G., Perham, R.N., (1978) Biochemistry, 17 (11), pp. 2189-2192 | ||
| 504 | |a Stroffekova, K., Proenza, C., Beam, K.G., (2001) Pflugers Archiv Eur J Physiol, 442 (6), pp. 859-866 | ||
| 504 | |a Tseng, C.H., (2004) Toxicol Appl Pharmacol, 197 (2), pp. 67-83 | ||
| 504 | |a Vizioli, N.M., Rusell, M.L., Carbajal, M.L., Carducci, C.N., Grasselli, M., (2005) Electrophoresis, 26 (15), pp. 2942-2948 | ||
| 504 | |a Yeh, A.P., McMillan, A., Stowell, M.H., Acta crystallographica section d (2006) Biol Crystallogr, 62 (PART 4), pp. 451-457 | ||
| 504 | |a Zhang, X., Yang, F., Shim, J.Y., Kirk, K.L., Anderson, D.E., Chen, X., (2007) Cancer Lett, 255 (1), pp. 95-106 | ||
| 520 | 3 | |a Arsenic-binding proteins are under continuous research. Their identification and the elucidation of arsenic/ protein interaction mechanisms are important because the biological effects of these complexes may be related not only to arsenic but also to the arsenic/protein structure. Although many proteins bearing a CXXC motif have been found to bind arsenic in vivo, new tools are necessary to identify new arsenic targets and allow research on protein/ arsenic complexes. In this work, we analyzed the performance of the fluorescent compound APAO-FITC (synthesized from p-aminophenylarsenoxide, APAO, and fluorescein isothiocyanate, FITC) in arsenic/protein binding assays using thioredoxin 1 (Trx) as an arsenic-binding protein model. The Trx-APAO-FITC complex was studied through different spectroscopic techniques involving UV-Vis, fluorescence, atomic absorption, infrared and circular dichroism. Our results show that APAO-FITC binds efficiently and specifically to the Trx binding site, labeling the protein fluorescently, without altering its structure and activity. In summary, we were able to study a protein/ arsenic complex model, using APAO-FITC as a labeling probe. The use of APAO-FITC in the identification of different protein and cell targets, as well as in in vivo biodistribution studies, conformational studies of arsenicbinding proteins, and studies for the design of drug delivery systems for arsenic anti-cancer therapies, is highly promising. © Springer Science+Business Media, LLC 2012. |l eng | |
| 536 | |a Detalles de la financiación: Universidad Nacional de Quilmes, PUNQ0396/07, UNQ-MPBio 53/1001 | ||
| 536 | |a Detalles de la financiación: Consejo Nacional de Investigaciones Científicas y Técnicas, PIP 5832/05 | ||
| 536 | |a Detalles de la financiación: Ministerio de Ciencia, Tecnología e Innovación Productiva, PME 01730 | ||
| 536 | |a Detalles de la financiación: Acknowledgments We want to thank Dr. Gerardo Caballero for helpful discussions with mass spectra analyses, and to Lic Martín Noguera for his assistance with CD experiments. This work was supported by grants from Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET; PIP 5832/05) and Universidad Nacional de Quilmes (UNQ; UNQ-MPBio 53/1001(PUNQ0396/07), MINCyT (PME 01730). S del VA, MLC and MG are members of CONICET (Scientific Research Program) and IMBICE. Fellowships for ALF (CONICET, Posgrado T-I and II, D. No. Res. 1086/09), CFT (CONICET, Posgrado T-I and II, D. No. Res. 3100/08) and MSA (UNQ, No. Res. (CS) 113/07) are acknowledged. | ||
| 593 | |a Laboratorio de Biomembranas, Departamento de Ciencia y Tecnología, Universidad Nacional de Quilmes, Roque Saenz Peña 352, Bernal, B1876BXD Buenos Aires, Argentina | ||
| 593 | |a Laboratorio de Inmunología y Virología, Departamento de Ciencia y Tecnología, Universidad Nacional de Quilmes, Roque Saenz Peña 352, Bernal, B1876BXD Buenos Aires, Argentina | ||
| 593 | |a Instituto de Bioquímica y Biofísica (IQUIFIB), Departamento de Química Biológica, Universidad de Buenos Aires, Buenos Aires, Argentina | ||
| 593 | |a LaMaBio, Departamento de Ciencia y Tecnología, Universidad Nacional de Quilmes, Roque Saenz Peña 352, Bernal, B1876BXD Buenos Aires, Argentina | ||
| 690 | 1 | 0 | |a ARSENIC CARCINOGENESIS |
| 690 | 1 | 0 | |a CIRCULAR DICHROISM |
| 690 | 1 | 0 | |a CONFORMATIONAL STUDIES |
| 690 | 1 | 0 | |a DRUG DELIVERY SYSTEMS |
| 690 | 1 | 0 | |a THIOREDOXIN |
| 690 | 1 | 0 | |a 4 AMINOPHENYLARSINE OXIDE |
| 690 | 1 | 0 | |a ARSENIC |
| 690 | 1 | 0 | |a ARSENIC BINDING PROTEIN |
| 690 | 1 | 0 | |a BINDING PROTEIN |
| 690 | 1 | 0 | |a FLUORESCEIN ISOTHIOCYANATE |
| 690 | 1 | 0 | |a FLUORESCENT DYE |
| 690 | 1 | 0 | |a THIOREDOXIN 1 |
| 690 | 1 | 0 | |a UNCLASSIFIED DRUG |
| 690 | 1 | 0 | |a ARTICLE |
| 690 | 1 | 0 | |a ATOMIC ABSORPTION |
| 690 | 1 | 0 | |a CIRCULAR DICHROISM |
| 690 | 1 | 0 | |a ENZYME ACTIVITY |
| 690 | 1 | 0 | |a FLUORESCENCE |
| 690 | 1 | 0 | |a POLYACRYLAMIDE GEL ELECTROPHORESIS |
| 690 | 1 | 0 | |a PROTEIN FOLDING |
| 690 | 1 | 0 | |a PROTEIN INTERACTION |
| 690 | 1 | 0 | |a PROTEIN STRUCTURE |
| 690 | 1 | 0 | |a ARSENIC |
| 690 | 1 | 0 | |a ARSENICALS |
| 690 | 1 | 0 | |a CARRIER PROTEINS |
| 690 | 1 | 0 | |a FLUORESCEIN-5-ISOTHIOCYANATE |
| 690 | 1 | 0 | |a FLUORESCENT DYES |
| 690 | 1 | 0 | |a KINETICS |
| 690 | 1 | 0 | |a MODELS, MOLECULAR |
| 690 | 1 | 0 | |a PROTEIN BINDING |
| 690 | 1 | 0 | |a PROTEIN CONFORMATION |
| 690 | 1 | 0 | |a PROTEIN UNFOLDING |
| 690 | 1 | 0 | |a SPECTRUM ANALYSIS |
| 690 | 1 | 0 | |a TEMPERATURE |
| 690 | 1 | 0 | |a THIOREDOXINS |
| 700 | 1 | |a Temprana, C.F. | |
| 700 | 1 | |a Santos, J. | |
| 700 | 1 | |a Carbajal, M.L. | |
| 700 | 1 | |a Amor, M.S. | |
| 700 | 1 | |a Grasselli, M. | |
| 700 | 1 | |a Del Alonso, S.V. | |
| 773 | 0 | |d 2012 |g v. 31 |h pp. 656-666 |k n. 8 |p Protein J. |x 15723887 |w (AR-BaUEN)CENRE-5767 |t Protein Journal | |
| 856 | 4 | 1 | |u https://www.scopus.com/inward/record.uri?eid=2-s2.0-84871453073&doi=10.1007%2fs10930-012-9441-6&partnerID=40&md5=f6cb9c3e7a418439daab74ed9c999e72 |y Registro en Scopus |
| 856 | 4 | 0 | |u https://doi.org/10.1007/s10930-012-9441-6 |y DOI |
| 856 | 4 | 0 | |u https://hdl.handle.net/20.500.12110/paper_15723887_v31_n8_p656_Femia |y Handle |
| 856 | 4 | 0 | |u https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_15723887_v31_n8_p656_Femia |y Registro en la Biblioteca Digital |
| 961 | |a paper_15723887_v31_n8_p656_Femia |b paper |c PE | ||
| 962 | |a info:eu-repo/semantics/article |a info:ar-repo/semantics/artículo |b info:eu-repo/semantics/publishedVersion | ||
| 999 | |c 84254 | ||