A chromosomal cluster of genes encoding ADP-glucose synthetase, glycogen synthase and phosphoglucomutase in Agrobacterium tumefaciens
A chromosomal region from Agrobacterium tumefaciens that complements exoC (pgm) mutations was cloned and sequenced. A cluster of three open reading frames (ORF1, ORF2 and ORF3) was identified. These genes are oriented in the same direction and are involved in the synthesis of glycogen and other poly...
Guardado en:
| Autor principal: | |
|---|---|
| Otros Autores: | |
| Formato: | Capítulo de libro |
| Lenguaje: | Inglés |
| Publicado: |
1994
|
| Acceso en línea: | Registro en Scopus DOI Handle Registro en la Biblioteca Digital |
| Aporte de: | Registro referencial: Solicitar el recurso aquí |
| Sumario: | A chromosomal region from Agrobacterium tumefaciens that complements exoC (pgm) mutations was cloned and sequenced. A cluster of three open reading frames (ORF1, ORF2 and ORF3) was identified. These genes are oriented in the same direction and are involved in the synthesis of glycogen and other polysaccharides. ORF1 encodes a 420-amino-acid (aa) protein with 55.9% homology to Escherichia coli GlgC (ADP-glucose synthetase, EC 2.7.7.27). ORF2 encodes a 480-aa protein with 42.2% homology to E. coli GlgA (glycogen synthase, EC 2.4.1.21). Based on Tn5 mutagenesis and protein homology, ORF3 was identified as the structural gene encoding phosphoglucomutase (Pgm, EC 2.7.5.1). ORF3 encodes a 542-aa protein with 52.6% homology to rabbit Pgm. There is no significant homology (less than 20%) to the Xanthomonas campestris XanA protein, which displays phosphomannomutase (Pmm) and Pgm activities [Koplin et al. J. Bacteriol 174 (1992) 191-199]. An A. tumefaciens pgm::Tn5 mutant retains Pmm activity. © 1994. |
|---|---|
| Bibliografía: | Auerswald, Ludwig, Schaller, Structural analysis of Tn5 (1981) Cold Spring Harbor Symp. Quant. Biol., 45, pp. 107-113 Bachmann, Linkage map of Escherichia coli K-12 (1983) Microbiol. Rev., 47, pp. 180-230. , Edition 7 Baecker, Furlong, Preiss, Biosynthesis of bacterial glycogen. Primary structure of Escherichia coli ADP-glucose synthetase as deduced from the nucleotide sequence of the glgC gene (1983) J. Biol. Chem., 258, pp. 5084-5088 Cangelosi, Hung, Puvanesarajah, Stacey, Ozga, Leigh, Nester, Common loci for Agrobacterium tumefaciens and Rhizobium meliloti exopolysaccharide synthesis and their roles in plant interactions (1987) J. Bacteriol., 169, pp. 2086-2091 Dell, York, McNeil, Darvil, Albersheim, The cyclic structure of β-d-(1,2)-linked D-glucans secreted by Rhizobia and Agrobacteria (1983) Carbohydr. Res., 117, pp. 185-200 Eidels, Edelmann, Preiss, Biosynthesis of bacterial glycogen, VIII. Activation and inhibition of the adenosine diphospho-glucose pyrophosphorilases of Rhodopseudomonas capsulata and of Agrobacterium tumefadens (1970) Arch. Biochem. Biophys., 140, pp. 60-74 Hisamatsu, Abe, Amemura, Harada, Structural elucidation of succinoglycan and related polysaccharides from Agrobacterium and Rhizobium by fragmentation with two special β-d-glycanases and methylation analysis (1980) Agricultural and Biological Chemistry, 44, pp. 1049-1055 Knauf, Nester, Wide host range cloning vectors: a cosmid clone bank of an Agrobacterium Ti plasmid (1982) Plasmid, 8, pp. 45-54 Kopiin, Arnold, Hotte, Simon, Wang, Fühler, Genetics of xanthan production in Xanthomonas campestris: the xanA and xanB genes are involved in UDP-glucose and GDP-mannose biosynthesis (1992) J. Bacteriol., 174, pp. 191-199 Kurnar, Larsen, Preiss, Biosynthesis of bacterial glycogen. Primary structure of Escherichia coli ADP-glucose: α-1,4-glucan, 4-glucosyltransferase as deduced from the nucleotide sequence of the gig A gene (1986) J. Biol. Chem., 261, pp. 16256-16259 Leigh, Signer, Walker, Exopolysaccharide-deficient mutants of Rhizobium meliloti that form ineffective nodules (1985) Proc. Natl. Acad. Sci. USA, 82, pp. 6231-6235 Leigh, Lee, Characterization of polysacccharides of Rhizobium mdiloti exo mutants that form ineffective nodules (1988) J. Bacteriol., 170, pp. 3327-3332 Lipman, Pearson, Rapid and sensitive protein similarity searches (1985) Science, 227, pp. 1435-1440 Madsen, The occurrence and enzymic synthesis of glycogen in extracts of Agrobacterium tumefaciens (1961) Biochim. Biophys. Acta, 50, pp. 194-195 Matthysse, Role of bacterial cellulose fibrils in Agrobacterium tumefaciens infection (1983) J. Bacteriol., 154, pp. 906-915 Preiss, Bacterial glycogen synthesis and its regulation (1984) Annu. Rev. Microbiol., 38, pp. 419-458 Ray, Jr., Peck, Jr., Phosphomutases (1972) The Enzymes, 6, pp. 407-477. , P.D. Boyer, Academic Press, New York, NY Ray, Jr., Hermodson, Puvathingal, Mahoney, The complete amino acid sequence of rabbit muscle phosphoglucomutase (1983) J. Biol. Chem., 258, pp. 9166-9174 Reuber, Walker, Biosynthesis of succinoglycan, a symbiotically important exoplysaccharide of Rhizobium meliloti (1993) Cell, 74, pp. 269-280 Rüssel, Kidd, Kelley, An improved filamentous helper phage for generating single-stranded plasmid DNA (1986) Gene, 45, pp. 333-338 Sanderson, Roth, Linkage map of Salmonella typhimurium (1983) Microbiol. Rev., 47, pp. 410-453. , edition VI Uttaro, Cangelosi, Geremia, Nester, Ugalde, Biochemical characterization of avirulent exoC mutants of Agrobacterium tumefaciens (1990) J. Bacteriol., 172, pp. 1640-1646 Uttaro, lelpi, Ugalde, Galactose metabolism in Rhizobiaceae; characterization of Agrobacterium tumefaciens exoB mutants (1993) J. Gen. Microbiol., 139, pp. 1055-1062 Weickert, Adhya, The galactose regulon of Escherichia coli (1993) Mol. Microbiol., 10, pp. 245-251 Whitehouse, Putt, Lovegrove, Morrison, Hollyoake, Fox, Hopkinson, Edwards, Phosphoglucomutase 1: complete human and rabbit mRNA sequences and direct mapping of this highly polymorphic marker on human chromosome 1 (1992) Proc. Natl. Acad. Sci. USA, 89, pp. 411-415 Zevenhuizen, Cellular glycogen, β-( 1,2)-glucan, poly-βhydroxybutyric acid and extracellular polysaccharides in fast growing species of Rhizobium (1981) Antonie van Leeuwenhoek J. Microbiol. Serol., 47, pp. 481-497 |
| ISSN: | 03781119 |
| DOI: | 10.1016/0378-1119(94)90869-9 |