Ripening-Associated Microstructural Changes in Antisense ACC Synthase Tomato Fruit
The ultrastructural impact of low ethylene biosynthesis (less than 0.5% of normal levels) was evaluated in transgenic (A11.1) tomatoes (Lycopersicon esculentumMill.) expressing an antisense 1-aminocyclopropane-1-carboxylic acid synthase (ACC-S) transgene by means of transmission and environmental sc...
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| Formato: | Capítulo de libro |
| Lenguaje: | Inglés |
| Publicado: |
2001
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| Acceso en línea: | Registro en Scopus DOI Handle Registro en la Biblioteca Digital |
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| Sumario: | The ultrastructural impact of low ethylene biosynthesis (less than 0.5% of normal levels) was evaluated in transgenic (A11.1) tomatoes (Lycopersicon esculentumMill.) expressing an antisense 1-aminocyclopropane-1-carboxylic acid synthase (ACC-S) transgene by means of transmission and environmental scanning electron microscopy. In 48-day mature green fruit, no significant ultrastructural differences were found between transgenic and control tomatoes. In 78-day control fruit, which were overripe and showed deteriorated texture, many areas of the cytoplasm were devoid of structures, and micrographs showed cell collapse with folding and dissolution of the cell wall. On the other hand, in 90-day transgenic fruit, which were firm and not ripe, the cytoplasm showed a relatively high electron density. Plastids retained remnants of chloroplast thylakoids along with significant amounts of osmiophylic plastoglobuli, but lycopene was not detected. Conspicuous starch granules were observed in mature green transgenic tomatoes, but were not detected in 90-day chlorochromoplasts. Electron-dense regions reflecting the integrity of the middle lamella alternated with other partially degraded regions. This incipient dissolution of the middle lamella pectic polymers may be attributable to nonenzymatic deaggregation or to cell-wall hydrolases which could be ethylene independent or responsive to very low levels of ethylene. Besides, cells were attached along extended contact areas and appeared turgid. This feature may provide an explanation of firmness retention that does not solely involve cell walls. Disruption of the middle lamella and development of lycopene crystalloids were observed when exogenous ethylene (12 ppm) was applied. © 2001, Sage Publications. All rights reserved. |
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| Bibliografía: | Barrett, D.M., Garcia, E., Wayne, J.E., Textural modification of processing tomatoes (1998) CRC Critical Reviews in Food Science and Nutrition, 38, pp. 173-258 Ben-Arie, R., Kislev, N., Frenkel, C., Ultrastructural changes in the cell walls of ripening apple and pear fruit (1979) Plant Physiology, 64, pp. 197-202 Brummell, D.A., Harpster, M.H., Civello, P.M., Palys, J.M., Bennett, A.B., Dunsmuir, P., Modification of expansin protein abundance in tomato fruit alters softening and cell wall polymer metabolism during ripening (1999) The Plant Cell, 11, pp. 2203-2216 Carey, A.T., Holt, K., Picard, S., Wilde, R., Tucker, G.A., Bird, C.R., Schuch, W., Seymour, G.B., Tomato exo-(1.4)- •-D-galactanase. Isolation, changes during ripening in normal and mutant tomato fruit, and characterization of a related cDNA clone (1995) Plant Physiology, 108, pp. 1099-1107 Carpita, N.C., Gibeaut, D.M., Structural models of primary cell walls in flowering plants: consistency of molecular structure with the physical properties of the walls during growth (1993) The Plant Journal, 3, pp. 1-30 Crookes, P.R., Grierson, D., Ultrastructure of tomato fruit ripening and the role of polygalacturonase isoenzymes in cell wall degradation (1983) Plant Physiology, 72, pp. 1088-1093 D’Souza, M.C., Singha, S., Ingle, M., Lycopene concentration of tomato fruit can be estimated from chromaticity values (1992) Hort Science, 27, pp. 465-466 Fishman, M.L., Gross, K.C., Gillespie, D.T., Sondey, S.M., Macromolecular components of tomato fruit pectin (1989) Archives of Biochemistry and Biophysics, 274, pp. 179-191 Fraschina, A.A., Cascone, O., Sozzi, G.O., Alpha-Larabinofuranosidase during growth and ripening of tomatoes expressing an antisense ACC synthase transgene (2000) Abstracts of IV International Conference on Postharvest Science, , Jerusalem, Israel. March 26–31, 2000 Fraser, P.D., Truesdale, M.R., Bird, C.R., Schuch, W., Bramley, P.M., Carotenoid biosynthesis during tomato fruit development. Evidence for tissue-specific gene expression (1994) Plant Physiology, 105, pp. 405-413 Hallett, I.C., MacRae, E.A., Wegrzyn, T.F., Changes in kiwifruit cell wall ultrastructure and cell packing during postharvest ripening (1992) International Journal of Plant Science, 153, pp. 49-60 Harris, W.M., Spurr, A.R., Chromoplasts of tomato fruits. I. Ultrastructure of low-pigment and high-beta mutants. Carotene analyses (1969) American Journal of Botany, 56, pp. 369-379 Harris, W.M., Spurr, A.R., Chromoplasts of tomato fruits. II. The red tomato (1969) American Journal of Botany, 56, pp. 380-389 Hendry, G.A.F., Houghton, J.D., Brown, S.B., The degradation of chlorophyll—A biological enigma (1987) New Phytologist, , 107: 255–302 Hobson, G., Grierson, D., Tomato (1993) Biochemistry of Fruit Ripening, pp. 405-442. , In: Seymour G.B., Taylor J.E. and Tucker G.A. (eds), University Press, Cambridge: Chapman and Hall Kobayashi, H., Differentiation of amyloplasts and chromoplasts (1991) The Photosynthetic Apparatus. Molecular Biology and Operation, pp. 395-415. , In: Bogorad L. and Vasil I.K. (eds.), New York: Academic Press Lichtenthaler, H.K., Chlorophylls and carotenoids: pigments of photosynthetic biomembranes (1987) Methods in Enzymology, 148, pp. 350-382 Maclachlan, G., Brady, C., Endo-1,4- •-glucanase, xyloglucanase and xyloglucan endo-transglycosylase activities versus potential substrates in ripening tomatoes (1994) Plant Physiology, 105, pp. 965-974 Mignani, I., Greve, C., Ben-Arie, R., Stotz, H.U., Li, C., Shackel, K.A., Labavitch, J.M., The effects of GA 3and divalent cations on aspects of pectin metabolism and tissue softening in ripening tomato pericarp (1995) Physiologia Plantarum, 93, pp. 108-115 Mohr, W.P., Pigment bodies in fruits of crimson and high pigment lines of tomatoes (1979) Annals of Botany, 44, pp. 427-434 Oeller, P.W., Min-Wong, L., Taylor, L., Pike, D.A., Theologis, A., Reversible inhibition of tomato fruit senescence by antisense RNA (1991) Science, 254, pp. 437-439 Orfila, C., Knox, J.P., Spatial regulation of pectic polysaccharides in relation to pit fields in cell walls of tomato fruit pericarp (2000) Plant Physiology, 122, pp. 775-781 Pesis, E., Fuchs, Y., Zaubermann, G., Cellulase activity and fruit softening in avocado (1978) Plant Physiology, 61, pp. 416-417 Platt-Aloia, K.M., Thomson, W.W., Young, R.E., Ultrastructural changes in the walls of ripening avocados: transmission, scanning and freeze fracture microscopy (1980) Botanical Gazette, 141, pp. 366-373 Platt-Aloia, K.M., Thomson, W.W., Ultrastructure of the mesocarp of mature avocado fruit and changes associated with ripening (1981) Annals of Botany, 48, pp. 451-465 Pressey, R., •-Galactosidases in ripening tomatoes (1983) Plant Physiology, 71, pp. 132-135 Rottmann, W.H., Peter, G.F., Oeller, P.W., Keller, J.A., Shen, N.F., Nagy, B.P., Taylor, L.P., Theologis, A., 1-Aminocyclopropane-1-carboxylate synthase in tomato is encoded by a multigene family whose transcription is induced during fruit and flower senescence (1991) Journal of Molecular Biology, 222, pp. 937-961 Seymour, G.B., Gross, K.C., Cell wall disassembly and fruit softening (1996) Postharvest News and Information, 7, pp. 45N-52N Shackel, K.A., Greve, L.C., Labavitch, J.M., Ahmadi, H., Cell turgor changes associated with ripening in tomato pericarp tissue (1991) Plant Physiology, 97, pp. 814-816 Sitrit, Y., Bennett, A.B., Regulation of tomato fruit polygalacturonase mRNA accumulation by ethylene: a reexamination (1998) Plant Physiology, 116, pp. 1145-1150 Smith, C.J.S., Watson, C.F., Morris, P.C., Bird, C.R., Seymour, G.B., Gray, J.E., Arnold, C., Grierson, D., Inheritance and effect on ripening of antisense polygalacturonase genes in transgenic tomatoes (1990) Plant Molecular Biology, 14, pp. 369-379 Smith, D.L., Starret, D.A., Gross, K.C., A gene coding for tomato fruit •-galactosidase II is expressed during fruit ripening. Cloning, characterization, and expression pattern (1998) Plant Physiology, 117, pp. 417-423 Sozzi, G.O., Fraschina, A.A., Evaluation of sensory attributes and biochemical parameters in transgenic tomato fruit with reduced polygalacturonase activity (1997) Food Science and Technology International, 3, pp. 93-102 Sozzi, G.O., Camperi, S.A., Cascone, O., Fraschina, A.A., Galactosidases in tomato fruit ontogeny: decreased galactosidase activities in antisense ACC synthase fruit during ripening and reversal with exogenous ethylene (1998) Australian Journal of Plant Physiology, 25, pp. 237-244 Spurr, A.R., A low-viscosity epoxy resin embedding medium for electron microscopy (1969) Journal of Ultrastructural Research, 26, pp. 31-43 Steele, N.M., McCann, M.C., Roberts, K., Pectin modification in cell walls of ripening tomatoes occurs in distinct domains (1997) Plant Physiology, 114, pp. 373-381 Su, L., McKeon, T., Grierson, D., Cantwell, M., Yang, S.F., Development of 1-aminocyclopropane-1-carboxylic acid synthase and polygalacturonase activities during the maturation and ripening of tomato fruit (1984) Hort Science, 19, pp. 576-578 Trinchero, G.D., Sozzi, G.O., Cerri, A.M., Vilella, F., Fraschina, A.A., Ripening-related changes in ethylene production, respiration rate and cell-wall enzyme activity in goldenberry (Physalis peruviana L.), a solanaceous species (1999) Postharvest Biology and Technology, , 16: 139–145 Tucker, G.A., Introduction (1993) Biochemistry of Fruit Ripening, pp. 1-51. , In: Seymour G.B., Taylor J.E. and Tucker G.A. (eds.), University Press, Cambridge: Chapman and Hall Yang, S.F., Hoffman, N.E., Ethylene biosynthesis and its regulation in higher plants (1984) Annual Review of Plant Physiology, 35, pp. 155-189 Zarembinski, T.I., Theologis, A., Ethylene biosynthesis and action: a case of conservation (1994) Plant Molecular Biology, 26, pp. 1579-1597 |
| ISSN: | 10820132 |
| DOI: | 10.1106/MARW-6NUR-GU3H-D2EL |