The glycoconjugates from Trypanosoma cruzi, purification and composition of a glycoprotein
1. 1. The complex of glycoconjugates extracted with phenol-water from culture epimastigotes of a non-infective line (To) of Trypanosoma cruzi, derived from the Tulahuén strain showed only two components by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. 2. 2. The slower migrating glycocon...
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1982
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| Acceso en línea: | Registro en Scopus DOI Handle Registro en la Biblioteca Digital |
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| LEADER | 07169caa a22008417a 4500 | ||
|---|---|---|---|
| 001 | PAPER-1237 | ||
| 003 | AR-BaUEN | ||
| 005 | 20230518203035.0 | ||
| 008 | 190411s1982 xx ||||fo|||| 00| 0 eng|d | ||
| 024 | 7 | |2 scopus |a 2-s2.0-0020069441 | |
| 024 | 7 | |2 cas |a Carbohydrates; Glycoproteins; Macromolecular Systems | |
| 040 | |a Scopus |b spa |c AR-BaUEN |d AR-BaUEN | ||
| 100 | 1 | |a de Lederkremer, R.M. | |
| 245 | 1 | 4 | |a The glycoconjugates from Trypanosoma cruzi, purification and composition of a glycoprotein |
| 260 | |c 1982 | ||
| 270 | 1 | 0 | |m de Lederkremer, R.M.; Departamento de Quimica Orgánica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Pabellon 2, Ciudad Universitaria, 1428 Buenos Aires, Argentina |
| 506 | |2 openaire |e Política editorial | ||
| 504 | |a Alves, Colli, Glycoproteins from Trypanosoma cruzi: partial purification by gel chromatography (1975) FEBS Lett., 52, pp. 188-190 | ||
| 504 | |a Davidson, Analysis of sugars found in mucopolysaccharides (1966) Methods in Enzymology, 8, pp. 52-60. , E.F. Neufeld, V. Ginsburg, Academic Press, New York | ||
| 504 | |a Dubois, Gilles, Hamilton, Rebers, Smith, Colorimetric method for determination of sugars and related substances (1956) Anal. Chem., 28, pp. 350-356 | ||
| 504 | |a Fairbanks, Steck, Wallach, Electrophoretic analysis of the major polypeptides of the human erythrocyte membrane (1971) Biochemistry, 10, pp. 2606-2616 | ||
| 504 | |a Gottlieb, Trypanosoma cruzi: identification of a cell surface polysaccharide (1978) Exp. Parasitol., 45, pp. 200-207 | ||
| 504 | |a Hough, Jones, Wadman, Quantitative analysis of mixtures of sugars by the method of partition chromatography Part V Improved methods for the separation and detection of the sugars and their methylated derivatives on the paper chromatogram (1950) Journal of the Chemical Society (Resumed), p. 1702 | ||
| 504 | |a Korn, Wright, Macromolecular composition of an amoeba plasma membrane (1973) J. biol. Chem., 248, pp. 439-447 | ||
| 504 | |a Lederkremer, Alves, Fonseca, Colli, A lipopeptidophosphoglycan from Trypanosoma cruzi (epimastigota) Isolation purification and carbohydrate composition (1976) Biochimica et Biophysica Acta (BBA) - General Subjects, 444, pp. 85-96 | ||
| 504 | |a Lederkremer, Tanaka, Alves, Colli, Lipopeptidophosphoglycan from Trypanosoma cruzi (1977) Amide and ester linked fatty acids, 74, pp. 263-267. , Eur. J. Biochem | ||
| 504 | |a Lederkremer, Casal, Tanaka, Colli, Ceramide and inositol content of the lipopeptidophosphoglycan from Trypanosoma cruzi (1978) Biochem. Biophys. Res. Commun., 85, pp. 1268-1274 | ||
| 504 | |a Lowry, Rosenbrough, Farr, Randall, Protein measurement with Folin phenol reagent (1951) J. biol. Chem., 193, pp. 265-275 | ||
| 504 | |a Scott, Snary, Protective immunisation of mice using cell surface glycoprotein from Trypanosoma cruzi (1979) Nature, Lond., 282, pp. 73-74 | ||
| 504 | |a Segrest, Jackson, Molecular weight determination of glycoproteins by polyacrylamide gel electrophoresis in sodium dodecyl sulfate (1972) Methods in Enzymology, 28, pp. 54-63. , S.P. Colowick, N.O. Kaplan, Academic Press, New York | ||
| 504 | |a Segura, Cura, Paulone, Vazquez, Cerisola, Antigenic makeup of subcellular fractions of Trypanosoma cruzi (1974) J. Protozool., 21, pp. 571-574 | ||
| 504 | |a Snary, Hudson, Trypanosoma cruzi cell surface proteins: identification of one major glycoprotein (1979) FEBS Lett., 100, pp. 166-170 | ||
| 504 | |a Snary, Trypanosoma cruzi: antigenic invariance of the cell surface glycoprotein (1980) Exp. Parasitol., 49, pp. 68-77 | ||
| 504 | |a Toennies, Kolb, Techniques and reagents for paper chromatography (1951) Anal. Chem., 23, pp. 823-826 | ||
| 504 | |a Trevelyan, Procter, Harrison, Detection of sugars on paper chromatograms (1950) Nature, Lond., 166, pp. 444-445 | ||
| 504 | |a Vickerman, Antigenic variation in trypanosomes (1978) Nature, Lond., 273, pp. 613-617 | ||
| 520 | 3 | |a 1. 1. The complex of glycoconjugates extracted with phenol-water from culture epimastigotes of a non-infective line (To) of Trypanosoma cruzi, derived from the Tulahuén strain showed only two components by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. 2. 2. The slower migrating glycoconjugate, a glycoprotein of molecular weight 45,000 was purified to apparent homogeneity. 3. 3. A content of 76% protein, 17% neutral sugars and 6% glucosamine was analysed. Galactose and mannose occur in a ratio of 3:1 as determined by gas-liquid chromatography. A trace of glucose was detected in some samples. © 1982. |l eng | |
| 536 | |a Detalles de la financiación: Consejo Nacional de Investigaciones Científicas y Técnicas | ||
| 536 | |a Detalles de la financiación: Secretaria de Estado de Ciência e Tecnologia, SECT, PNUD/UNESCO RLA 78/024 | ||
| 536 | |a Detalles de la financiación: Acknowledyements--This investigation received financial support from Secretaria de Estado de Ciencia y Tecnologia (SECYT); project PNUD/UNESCO RLA 78/024 to RML. RML is a research member of the Consejo Nacional de Investigaciones Cientificas y T6cnicas de la Repdblica Argentina. We thank Dr. J. J. Cazzulo for helpful discussion and Dr. W. Colli (S~o Paulo, Brasil) for a sample of the glycoconjugate complex isolated from T. cruzi (Y strain). | ||
| 593 | |a Departamento de Quimica Orgánica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Pabellon 2, Ciudad Universitaria, 1428 Buenos Aires, Argentina | ||
| 593 | |a Instituto Fatala Chabén, Paseo Colón 568, 1063 Buenos Aires, Argentina | ||
| 690 | 1 | 0 | |a CARBOHYDRATE |
| 690 | 1 | 0 | |a GLYCOPROTEIN |
| 690 | 1 | 0 | |a ANIMAL |
| 690 | 1 | 0 | |a ARTICLE |
| 690 | 1 | 0 | |a GAS CHROMATOGRAPHY |
| 690 | 1 | 0 | |a ISOLATION AND PURIFICATION |
| 690 | 1 | 0 | |a MACROMOLECULE |
| 690 | 1 | 0 | |a MOLECULAR WEIGHT |
| 690 | 1 | 0 | |a POLYACRYLAMIDE GEL ELECTROPHORESIS |
| 690 | 1 | 0 | |a TRYPANOSOMA CRUZI |
| 690 | 1 | 0 | |a ANIMAL |
| 690 | 1 | 0 | |a CARBOHYDRATES |
| 690 | 1 | 0 | |a CHROMATOGRAPHY, GAS |
| 690 | 1 | 0 | |a ELECTROPHORESIS, POLYACRYLAMIDE GEL |
| 690 | 1 | 0 | |a GLYCOPROTEINS |
| 690 | 1 | 0 | |a MACROMOLECULAR SYSTEMS |
| 690 | 1 | 0 | |a MOLECULAR WEIGHT |
| 690 | 1 | 0 | |a SUPPORT, NON-U.S. GOV'T |
| 690 | 1 | 0 | |a TRYPANOSOMA CRUZI |
| 700 | 1 | |a Waksman, N. | |
| 700 | 1 | |a de Martini, G.J.W. | |
| 700 | 1 | |a Segura, E.L. | |
| 773 | 0 | |d 1982 |g v. 72 |h pp. 313-316 |k n. 2 |x 03050491 |w (AR-BaUEN)CENRE-2752 |t Comparative Biochemistry and Physiology -- Part B: Biochemistry and | |
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| 856 | 4 | 0 | |u https://doi.org/10.1016/0305-0491(82)90052-9 |y DOI |
| 856 | 4 | 0 | |u https://hdl.handle.net/20.500.12110/paper_03050491_v72_n2_p313_deLederkremer |y Handle |
| 856 | 4 | 0 | |u https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03050491_v72_n2_p313_deLederkremer |y Registro en la Biblioteca Digital |
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| 962 | |a info:eu-repo/semantics/article |a info:ar-repo/semantics/artículo |b info:eu-repo/semantics/publishedVersion | ||
| 963 | |a VARI | ||
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