Stress - induced expression of IPT gene in transgenic wheat reduces grain yield penalty under drought

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Background: The heterologous expression of isopentenyl transferase (IPT) under the transcriptional control of the senescence-associated receptor-like kinase (SARK) promoter delayed cellular senescence and, through it, increased drought tolerance in plants. To evaluate the effect of pSARK::IPT expres...

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Detalles Bibliográficos
Otros Autores: Beznec, Ailin, Faccio, Paula, Miralles, Daniel Julio, Abeledo, Leonor Gabriela, Oneto, Cecilia Décima, Garibotto, María de Belén, González, Germán Andrés, Moreyra, Federico
Formato: Artículo
Lenguaje:Inglés
Materias:
AGRONOMIC TRAITS
CYTOKININ
GENETIC TRANSFORMATION
ISOPENTENYL TRANSFERASE GENE
WATER DEFICIT TOLERANCE
TRITICUM AESTIVUM L
Acceso en línea:http://ri.agro.uba.ar/files/download/articulo/2021beznec.pdf
LINK AL EDITOR
Aporte de:Registro referencial: Solicitar el recurso aquí
Biblioteca Central (FAUBA) de Universidad de Buenos Aires
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245 1 |a Stress - induced expression of IPT gene in transgenic wheat reduces grain yield penalty under drought 
520 |a Background: The heterologous expression of isopentenyl transferase (IPT) under the transcriptional control of the senescence-associated receptor-like kinase (SARK) promoter delayed cellular senescence and, through it, increased drought tolerance in plants. To evaluate the effect of pSARK::IPT expression in bread wheat, six independent transgenic events were obtained through the biolistic method and evaluated transgene expression, phenology, grain yield and physiological biomass components in plants grown under both drought and well-irrigating conditions. Experiments were performed at different levels: (i) pots and (ii) microplots inside a biosafety greenhouse, as well as under (iii) field conditions. Results: Two transgenic events, called TR1 and TR4, outperformed the wild-type control under drought conditions. Transgenic plants showed higher yield under both greenhouse and field conditions, which was positively correlated to grain number (given by more spikes and grains per spike) than wild type. Interestingly, this yield advantage of the transgenic events was observed under both drought and well-watered conditions. Conclusions: The results obtained allow us to conclude that the SARK promoter-regulated expression of the IPT gene in bread wheat not only reduced the yield penalty produced by water stress but also led to improved productivity under well-watered conditions. 
650 |2 Agrovoc  |9 26 
653 |a AGRONOMIC TRAITS 
653 |a CYTOKININ 
653 |a GENETIC TRANSFORMATION 
653 |a ISOPENTENYL TRANSFERASE GENE 
653 |a WATER DEFICIT TOLERANCE 
653 |a TRITICUM AESTIVUM L 
700 1 |a Beznec, Ailin  |u Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética Edwald A. Favret. Buenos Aires, Argentina.  |9 73882 
700 1 |a Faccio, Paula  |u Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética, Edwald A. Favret. Buenos Aires, Argentina.  |9 73883 
700 1 |a Miralles, Daniel Julio  |u Universidad de Buenos Aires. Facultad de Agronomía. Departamento de Producción Vegetal. Cátedra de Cerealicultura. Buenos Aires, Argentina.  |u Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura (IFEVA). Buenos Aires, Argentina.  |u CONICET – Universidad de Buenos Aires. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura (IFEVA). Buenos Aires, Argentina.  |9 6438 
700 1 |a Abeledo, Leonor Gabriela  |u Universidad de Buenos Aires. Facultad de Agronomía. Departamento de Producción Vegetal. Cátedra de Cerealicultura. Buenos Aires, Argentina.  |9 11135 
700 1 |a Oneto, Cecilia Décima  |u Instituto Nacional de Tecnología Agropecuaria (INTA). Centro Regional Buenos Aires Sur. Estación Experimental Agropecuaria Balcarce (EEA Balcarce). Laboratorio de Agrobiotecnología. Buenos Aires, Argentina.  |u CONICET. Buenos Aires, Argentina.  |9 48731 
700 1 |a Garibotto, María de Belén  |u Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética Edwald A. Favret. Buenos Aires, Argentina.  |u CONICET. Buenos Aires, Argentina.  |9 73884 
700 1 |a González, Germán Andrés  |u Instituto Nacional de Tecnología Agropecuaria (INTA). Centro Regional Buenos Aires Sur. Estación Experimental Agropecuaria Bordenave (EEA Bordenave). Buenos Aires, Argentina.  |9 73885 
700 1 |a Moreyra, Federico  |u Instituto Nacional de Tecnología Agropecuaria (INTA). Centro Regional Buenos Aires Sur. Estación Experimental Agropecuaria Bordenave (EEA Bordenave). Buenos Aires, Argentina.  |9 57715 
773 |t Journal of Genetics Engineering and Biotechnology  |g Vol.19 (2021), art.67, 17 p., grafs., tbls., fot. 
856 |f 2021beznec  |i en internet  |q application/pdf  |u http://ri.agro.uba.ar/files/download/articulo/2021beznec.pdf  |x ARTI202206 
856 |u http://www.springer.com/  |z LINK AL EDITOR 
942 |c ARTICULO 
942 |c ENLINEA 
976 |a AAG 

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