Novel methods to induce exogenous gene expression in SCNT, parthenogenic and IVF preimplantation bovine embryos

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The import of exogenous DNA [eDNA] from the cytoplasm to the nucleus represents a key intracellular obstacle for efficient gene delivery in mammalian cells. In this study, cumulus cells or oolemma vesicles previously incubated with eDNA, and naked eDNA were injected into the cytoplasm of MII oocytes...

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Detalles Bibliográficos
Otros Autores: Pereyra Bonnet, Federico, Bevacqua, Romina Jimena, La Rosa, Isabel, Sipowicz, Pablo, Radrizzani Helguera, Martín, Fernández Martín, Rafael, Salamone, Daniel Felipe
Formato: Artículo
Lenguaje:Inglés
Materias:
CLONED CATTLE PARTHENOGENESIS
GFP TRANSGENESIS
ENHANCED GREEN FLUORESCENT PROTEIN
GREEN FLUORESCENT PROTEIN
IONOMYCIN
ANIMAL
ANIMAL EMBRYO
CATTLE
CELL NUCLEUS TRANSPLANTATION
CONFOCAL MICROSCOPY
CULTURE MEDIUM
CUMULUS CELL
CYTOLOGY
CYTOPLASM
DRUG EFFECT
EMBRYO CULTURE
FLUORESCENCE IN SITU HYBRIDIZATION
GENE EXPRESSION
GENE EXPRESSION PROFILING
GENE TRANSFER
GENETICS
METABOLISM
METHODOLOGY
MICROINJECTION
OOCYTE
PARTHENOGENESIS
PLASMID
TIME
ANIMALS
CULTURE MEDIA
CUMULUS CELLS
DNA
EMBRYO CULTURE TECHNIQUES
EMBRYO, MAMMALIAN
FERTILIZATION IN VITRO
GENE TRANSFER TECHNIQUES
GREEN FLUORESCENT PROTEINS
IN SITU HYBRIDIZATION, FLUORESCENCE
MICROINJECTIONS
MICROSCOPY, CONFOCAL
NUCLEAR TRANSFER TECHNIQUES
OOCYTES
PLASMIDS
TIME FACTORS
BOS
BOVINAE
MAMMALIA
Acceso en línea:http://ri.agro.uba.ar/files/intranet/articulo/2011PereyraBonnet2.pdf
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Aporte de:Registro referencial: Solicitar el recurso aquí
Biblioteca Central (FAUBA) de Universidad de Buenos Aires
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